Sensation, mechanoreceptor, and nerve fiber function after nerve regeneration

Krarup, Christian; Rosén, Birgitta; Boeckstyns, Michel E. H.; Sörensen, Allan Ibsen; Lundborg, Göran; Moldovan, Mihai; Archibald, Simon J.

doi:10.1002/ana.25102

Cited by 14 publications

(16 citation statements)

References 49 publications

(180 reference statements)

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“…In this study, the incorporation of NFABNS into NeuraGen ® conduits prevented the deformation or compression of these commercial devices during the period analyzed. Deformation, compression and early reabsorption of some hollow conduits has been described associated to this technique or to the use of vein grafts in PNs reconstruction (Moore et al, 2009; Hernandez-Cortes et al, 2010; Wangensteen and Kalliainen, 2010; Daly W. et al, 2012; Liodaki et al, 2013; Papalia et al, 2013; Carriel et al, 2014a; Krarup et al, 2017). In this regard, engineered neural tissue-like substitutes based on cellular self-aligned and plastic compressed collagen hydrogels were covered by NeuraWrap TM (Integra, United States) devices and successfully used to repair a critical nerve gap of 15-mm in rats (Georgiou et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…In relation to the less favorable results obtained with filled NeuraGen ® conduits, our histological analyses suggest that this finding could be related to physical factors (Daly W. et al, 2012; Carriel et al, 2014a). It is well accepted that nerve conduits provide a close and controlled protective microenvironment that guide and support nerve tissue regeneration in non-critical nerve gaps (Daly W. et al, 2012; Carriel et al, 2013, 2014a; Krarup et al, 2017; Pedrosa et al, 2017; Ronchi et al, 2018). In this regard, we hypothesize that the combined use of an external conduit filled with a highly dense NFABNS may reduce the area needed for an optimal tissue regeneration, and this would explain the poor functional and electrophysiological recovery observed with this strategy.…”

Section: Discussionmentioning

confidence: 99%

“…The NeuraGen ® conduits were chosen due to their well-known positive impact on PNs regeneration when combined with FAH and ADMSCs (Carriel et al, 2013, 2017c). Furthermore, these conduits are FDA approved and they are currently one of the most frequently used nerve guides in the clinical practice (Wangensteen and Kalliainen, 2010; Krarup et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

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In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair

Chato‐Astrain

Campos

Roda

et al. 2018

Front. Cell. Neurosci.

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The regenerative capability of peripheral nerves is very limited, and several strategies have been proposed to increase nerve regeneration. In the present work, we have analyzed the in vivo usefulness of a novel nanostructured fibrin-agarose bio-artificial nerve substitute (Nano) used alone or in combination with NeuraGen® collagen type I conduits (Coll-Nano) in laboratory rats with a 10-mm sciatic nerve defect. Control animals were subjected to the gold-standard autograft technique (Auto). Results first demonstrated that the percentage of self-amputations was lower in Nano and Coll-Nano groups as compared to the Auto group. Neurotrophic ulcers were more abundant in the Auto group (60%, with 66.6% of them being >2-mm) than Nano and Coll-Nano groups (0%) at 4 weeks, although Nano showed more ulcers after 12 weeks. Foot length was significantly altered in Auto animals due to neurogenic retraction, but not in Nano and Coll-Nano groups after 12 weeks. At the functional level, all animals showed a partial sensory recovery as determined by the pinch test, especially in Nano and Auto groups, but did not reach the levels of native animals. Toe-spread test revealed a partial motor function recovery only in Nano animals at 4 weeks and Auto and Nano at 12 weeks. Electromyography showed clear denervation signs in all experimental groups, with few differences between Auto and Nano animals. After 12 weeks, an important denervation decrease and an increase of the reinnervation process was found in Auto and Nano groups, with no differences between these groups. Histological analyses demonstrated an active peripheral nerve regeneration process with newly formed peripheral nerve fascicles showing S-100, GAP-43 and myelin in all experimental groups. The peripheral nerve regeneration process was more abundant in Auto group, followed by Nano group, and both were better than Coll-Nano group. Muscle histology confirmed the electromyography results and showed some atrophy and fibrosis signs and an important weight and volume loss in all groups, especially in the Coll-Nano group (56.8% weight and 60.4% volume loss). All these results suggest that the novel Nano substitutes used in in vivo were able to contribute to bridge a 10-mm peripheral nerve defect in rats.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair

Chato‐Astrain

Campos

Roda

et al. 2018

Front. Cell. Neurosci.

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“…Recovery of touch sensation may be influenced by the number and function of regenerated fibres, while tactile gnosis requires the input and plasticity of the central nervous system 93. Furthermore, early re-learning can improve outcomes after median and ulnar nerve repair 94.…”

Section: Introductionmentioning

confidence: 99%

Update on corneal neurotisation

et al. 2018

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Corneal neurotisation describes surgical restoration of nerve growth into the cornea to restore corneal sensation and trophic function. It represents an exciting and effective emerging treatment for neurotrophic keratopathy. Techniques described to date involve either direct nerve transfer or an interpositional nerve graft coapted to a healthy donor nerve. We review the experience to date with particular emphasis on a detailed review of techniques, outcomes and current thoughts.

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“…Most of the attainable peripheral reinnervation is considered to be reached about 2 years after surgical repair of such an injury (Seddon et al, 1943;Sunderland, 1947;Buchthal and Kühl, 1979;Burnett and Zager, 2004;Grinsell and Keating, 2014). Residual innervation deficiencies involve loss of primary sensory and motor neurons, misdirected reinnervation of axons concerning location and type of end-organs, and abnormal conduction and stimulus coding properties of reinnervated neurons (Buchthal and Kühl, 1979;Hallin et al, 1981;Mackel et al, 1983;Liss et al, 1996;Allodi et al, 2012;de Ruiter et al, 2014;Krarup et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Disinhibition of Human Primary Somatosensory Cortex After Median Nerve Transection and Reinnervation

Nordmark

Johansson

2020

Front. Hum. Neurosci.

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Despite state-of-the-art surgical and postoperative treatment, median nerve transection causes lasting impaired hand function due to limitations in the nerve's reinnervation ability. The defective innervation and thus controllability of the affected hand can shape the brain's control of manual behaviors. Earlier studies of changes in the processing of tactile stimuli have focused mainly on stimulation of the reinnervated hand and lack sufficient control over the brain's use of the tactile input in perceptual terms. Here we used fMRI to measure brain activity (BOLD-signal) in 11 people with median nerve injury and healthy controls (N = 11) when performing demanding tactile tasks using the tip of either the index or little finger of either hand. For the nerve-injured group, the left median nerve had been traumatically transected in the distal forearm and surgically repaired on average 8 years before the study. The hand representation of their contralesional (right) primary somatosensory cortex (S1) showed greater activity compared to controls when the left reinnervated index finger was used, but also when the left-hand little finger and the fingers of the right hand innervated by uninjured nerves were used. We argue that the overall increase in activity reflects a general disinhibition of contralesional S1 consistent with an augmented functional reorganizational plasticity being an ongoing feature of chronic recovery from nerve injury. Also, the nerve-injured showed increased activity within three prefrontal cortical areas implicated in higher-level behavioral processing (dorsal anterior cingulate cortex, left ventrolateral prefrontal and right dorsolateral prefrontal cortex), suggesting that processes supporting decisionmaking and response-selection were computationally more demanding due to the compromised tactile sensibility.

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Sensation, mechanoreceptor, and nerve fiber function after nerve regeneration

Cited by 14 publications

References 49 publications

In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair

In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair

Update on corneal neurotisation

Disinhibition of Human Primary Somatosensory Cortex After Median Nerve Transection and Reinnervation

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