Senescent Accelerated Mouse (SAM): A Model that Binds <i>In Vivo</i> and <i>In Vitro</i> Aging

Yegorov, Yegor E.; Семенова, И. В.; Karachentsev, Dmitry; Semenova, M. L.; Akimov, S. S.; Yegorova, I. N.; Zelenin, A. V.

doi:10.1089/109454501750225677

Cited by 11 publications

(5 citation statements)

References 39 publications

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“…In recent experiments [40] on the cells of so‐called Japanese senescent accelerated mice, quite a good correlation between the mouse's longevity, the embryo fibroblast life spans in vitro and the time of senesced non‐dividing fibroblast survival in culture was found (Fig. 1 ).…”

Section: The Role Of Post‐mitotic Cells In Body Maintenancementioning

confidence: 80%

“…When the oxygen concentration in culture is reduced, there is a resultant increase in cell life [64,65]. As was mentioned above, the duration of survival of senesced cells is also dependent on the oxidative stress [60]. This fact to a certain degree serves to unite the fibroblast life span and the survival tests.…”

Section: Comparison Of Two Different Cellular Tests To Describe Organmentioning

confidence: 95%

“…For instance, among primates, humans have the longest life spans but the shortest telomeres [38]. On the other hand, rodents have a short life span and the longest telomeres [39,40]. Telomeres are even longer in SCID mice [41].…”

Section: Dependence Of the Cell Proliferative Life Span In Vitro On Omentioning

confidence: 99%

“…This problem may be overcome by growing cells under conditions of oxidative stress. It has been shown that the addition of hydrogen peroxide to the culture medium reduces the survival time, thus making it more practical to employ the test in investigations of organism ageing [60].…”

Section: The Role Of Post‐mitotic Cells In Body Maintenancementioning

confidence: 99%

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Duration of senescent cell survival in vitro as a characteristic of organism longevity, an additional to the proliferative potential of fibroblasts

Yegorov

Zelenin

2003

FEBS Letters

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More than 40 years have passed since the original publication by Hay£ick and Moorhead led to the concept of the 'Hay£ick limit' of the maximum number of divisions which somatic cells undergo in vitro. This concept is still regarded as a fundamental characteristic of species longevity. Here we want to emphasize another characteristic of somatic cells, namely, the duration of their survival in vitro in the non-dividing state after cessation of proliferation. This is suggested on the basis of results of recent experiments with so-called Japanese accelerated senescent mice. Results of these experiments reveal a good correlation between the longevity of the mice, the number of duplications of their ¢broblasts in vitro, and the survival time of these cells in the non-dividing state. In routine culture conditions, cell survival time may be very long, as much as a few years. However, when the cells are grown under conditions of oxidative stress, cellular longevity is markedly shortened. This new test may serve as an additional marker of organismic longevity. The comparative value of both tests, the classical 'Hay£ick limit' and the new test, is discussed.

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Section: The Role Of Post‐mitotic Cells In Body Maintenancementioning

confidence: 80%

Section: Comparison Of Two Different Cellular Tests To Describe Organmentioning

confidence: 95%

Section: Dependence Of the Cell Proliferative Life Span In Vitro On Omentioning

confidence: 99%

Section: The Role Of Post‐mitotic Cells In Body Maintenancementioning

confidence: 99%

See 2 more Smart Citations

Duration of senescent cell survival in vitro as a characteristic of organism longevity, an additional to the proliferative potential of fibroblasts

Yegorov

Zelenin

2003

FEBS Letters

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“…On the other hand, senescence study using in vivo models has been challenged by experimental constraints, such as the difficulty to identify senescent cells in a living organism, the lack of standardized and validated in vivo biomarkers, as well as the long-term monitoring needed for studying murine aging [158]; nevertheless, several systems for the study of senescence and its role in aging and cancer development in living rodents have been developed, including the use of several rat strains to characterize stromal cell senescence by ex vivo culture techniques [62,65,159,160], and using progeroid murine lines [161] and senescence accelerated mice (SAM) strains with different life spans and senescence associated features [162,163]. These mice display atypical expression of genes encoding cell cycle checkpoints, DNA repair and nuclear proteins, ROS scavenging enzymes and signaling pathways components and are considered valid models for studying both senescence and aging.…”

Section: In Vivo Rodent Modelsmentioning

confidence: 99%

In Vitro and In Vivo Modeling of Normal and Leukemic Bone Marrow Niches: Cellular Senescence Contribution to Leukemia Induction and Progression

Salazar-Terreros

Vernot

2022

IJMS

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Cellular senescence is recognized as a dynamic process in which cells evolve and adapt in a context dependent manner; consequently, senescent cells can exert both beneficial and deleterious effects on their surroundings. Specifically, senescent mesenchymal stromal cells (MSC) in the bone marrow (BM) have been linked to the generation of a supporting microenvironment that enhances malignant cell survival. However, the study of MSC’s senescence role in leukemia development has been straitened not only by the availability of suitable models that faithfully reflect the structural complexity and biological diversity of the events triggered in the BM, but also by the lack of a universal, standardized method to measure senescence. Despite these constraints, two- and three dimensional in vitro models have been continuously improved in terms of cell culture techniques, support materials and analysis methods; in addition, research on animal models tends to focus on the development of techniques that allow tracking leukemic and senescent cells in the living organism, as well as to modify the available mice strains to generate individuals that mimic human BM characteristics. Here, we present the main advances in leukemic niche modeling, discussing advantages and limitations of the different systems, focusing on the contribution of senescent MSC to leukemia progression.

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Human Senescence

Crews

2003

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Much research on the biology of senescence is on cell-lines, nematodes or fruit flies, that are only of peripheral relevance to the problems encountered in humans. Human Senescence is a 2003 text which reviews the evolutionary biology of human senescence and life span, and the evolutionarily recent development of late-life survival. It examines how human patterns of and variability in growth and development have altered later life survival probabilities and competencies, and how survival during mid-life contributes to senescent dysfunction and alteration. Discussing possibilities of further extending human life span, it gives a better understanding of how humans came to senesce as slowly as we do over our lifespan. Bringing together gerontological, anthropological and biocultural research, it explores human variation in chronic disease, senescence and life span as outcomes of early life adaptation and the success of humankind's sociocultural evolution. It is a benchmark publication for all interested in how and why we age.

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Senescent Accelerated Mouse (SAM): A Model that Binds In Vivo and In Vitro Aging

Cited by 11 publications

References 39 publications

Duration of senescent cell survival in vitro as a characteristic of organism longevity, an additional to the proliferative potential of fibroblasts

Duration of senescent cell survival in vitro as a characteristic of organism longevity, an additional to the proliferative potential of fibroblasts

In Vitro and In Vivo Modeling of Normal and Leukemic Bone Marrow Niches: Cellular Senescence Contribution to Leukemia Induction and Progression

Human Senescence

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