Senescence marker protein 30 (SMP30) serves as a potential prognostic indicator in hepatocellular carcinoma

Mo, Zhijing; Zheng, Shunxin; Lv, Zili; Zhuang, Yuan; Lan, Xiuwan; Wang, Feng; Lü, Xiaoling; Zhao, Yongxiang; Zhou, Sufang

doi:10.1038/srep39376

Cited by 17 publications

(14 citation statements)

References 27 publications

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“…Studies have indicated that DCs pulsed with recombinant fusion proteins composed of an antigenic fragment and HSP protein can stimulate CTL [ 15 ]. The literature also shows that HSP90 can stimulate DCs and induce CTL polarization and activation [ 16 ]. A study showed that cross-presenting DCs is highly effective in inducing CTLs capable of eliminating liver cancer [ 17 ].…”

Section: Results and Analysismentioning

confidence: 99%

GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

Huang

Pan

Zhang

et al. 2022

Journal of Healthcare Engineering

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Heat-shock protein (HSP) GP96 is a well-known adjuvant in immunotherapy. It belongs to the HSP90 family. Our previous study demonstrated that DC pulsed with recombinant senescence marker protein 30 (SMP30) could induce cytotoxic T lymphocytes (CTLs) against liver cancer cells in vitro. In this study, SMP30 and GP96 were subcloned into lentiviruses and transfected into DCs from healthy donors. We included six groups: the GP96-SMP30 group, GP96 group, SMP30 group, DC group, empty vector control group, and hepatoma extracted protein group. We used ELISA to detect cytokines and flow cytometry to assess CD80 and CD86 on DCs and the effect of CTLs. Our vector design was considered successful and further studied. In the SMP30 group, DC expresses more CCR7 and CD86 than the control group; in the SMP30+GP96 group, DC express more CCR7, CD86, and CD80 than the control group. Transfected DCs secreted more TNF-α and interferon-β and induced more CTLs than control DCs. SMP30 + GP96 effectively stimulated the proliferation of T cells compared with control treatment ( P < 0.01). We detected the cytokines TNF-α, TNF-β, IL-12, and IFN (α, β, and γ) via ELISA (Figure 5) and verified the killing effect via FCM. Four E : T ratios (0 : 1, 10 : 1, 20 : 1, and 40 : 1) were tested. The higher the ratio was, the better the effects were. We successfully constructed a liver cancer model and tested the CTL effect in each group. The GP96 + SMP30 group showed a better effect than the other groups. GP96 and SMP30 can stimulate DCs together and produce more potent antitumor effects. Our research may provide a new efficient way to improve the therapeutic effect of DC vaccines in liver cancer.

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Section: Results and Analysismentioning

confidence: 99%

GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

Huang

Pan

Zhang

et al. 2022

Journal of Healthcare Engineering

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“…Although the role of SMP30 and its concomitant expression are well documented in other tumors, such as hepatocellular carcinoma [ 53 ], the relationship between SMP30 and mammary gland tumors still remains ambiguous and controversial. Sar et al reported that high SMP30 expression is noted in MCF-7 cells [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Senescence Marker Protein 30 (SMP30): A Novel Pan-Species Diagnostic Marker for the Histopathological Diagnosis of Breast Cancer in Humans and Animals

Baek

Lee

Kim

et al. 2021

IJMS

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Senescence marker protein 30 (SMP30) is a cell survival factor playing an important role in vitamin C synthesis and antiapoptosis. Moreover, its cytoprotective role suggests a possibility to be related to cancer cell survival. Mammary carcinoma is a common cancer in both humans and animals. Because of its histopathological diversity, especially in the early stage, histopathological diagnosis may be complicated; therefore, a diagnostic marker is helpful for confirmation. The present study analyzed the expression pattern of SMP30 in mammary carcinoma in humans, dogs, and cats. Immunohistochemistry, immunofluorescence, and western blot analysis were used to investigate SMP30 expression patterns. The expression was specifically observed in neoplastic glandular epithelial cells. The expression increased with the malignancy of glandular epithelial cells with a highly proliferative status. However, SMP30 expression was low in normal mammary gland tissues or well-differentiated adenoma tissues. The patterns were consistently reproduced in canine primary mammary carcinoma cells and MCF-7 and MDA-MB-231 human carcinoma cell lines. This study provides useful information to understand SMP30 expression in various stages of mammary carcinoma and to suggest its utility as a pan-species diagnostic marker, thereby helping to establish strategies for diagnosing mammary carcinoma in several species.

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“…13 Moreover, SMP-30 serves as a prognostic marker and a potential therapeutic target for HCC. 14,15 It has been reported that SMP-30 expression is significantly reduced in HCC tissues compared with in adjacent non-tumor tissues and is negatively associated with the tumor size, TNM stage, and poor survival of HCC patients. 14 Silencing of SMP-30 promotes the proliferation and invasion of HepG2 cells.…”

Section: Introductionmentioning

confidence: 99%

“…14,15 It has been reported that SMP-30 expression is significantly reduced in HCC tissues compared with in adjacent non-tumor tissues and is negatively associated with the tumor size, TNM stage, and poor survival of HCC patients. 14 Silencing of SMP-30 promotes the proliferation and invasion of HepG2 cells. 15 Based on the antiaging effect of laminarin, we speculated that laminarin may upregulate SMP-30 expression.…”

Section: Introductionmentioning

confidence: 99%

Laminarin from Seaweed (Laminaria japonica) Inhibits Hepatocellular Carcinoma Through Upregulating Senescence Marker Protein-30

Lin

et al. 2020

Cancer Biotherapy and Radiopharmaceuticals

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Objective: This study aimed at investigating the specific roles of laminarin from seaweed (Laminaria japonica) in hepatocellular carcinoma (HCC) and its potential mechanisms related to senescence marker protein-30 (SMP-30). Materials and Methods: Human HCC cell lines, including Bel-7404 and HepG2, were incubated with different concentrations of laminarin (0, 5, 15, 25, 35, and 45 mg/mL). The cell viability and apoptosis rates were detected by WST-8 cell proliferation assay and flow cytometry, respectively. Hepa 1-6 tumor-bearing mice were injected with different concentrations of laminarin (400, 800, and 1200 mg/kg$d), and tumor volume and weight were measured. The expression of SMP-30 was detected in laminarin-treated Bel-7404 and HepG2 HCC cells and LO2 normal liver cells by quantitative real-time PCR and Western blotting. Results: The treatment with laminarin (48 h) significantly decreased the viability and increased the apoptosis rates of Bel-7404 and HepG2 cells in a dose-dependent manner. The injection of laminarin also significantly decreased the tumor volumes (beginning on the 10th day) and tumor weights (30 d post-injection) of mice in a dose-dependent manner. In addition, the treatment with laminarin (35 mg/mL for 48 h) significantly upregulated SMP-30 in Bel-7404 and HepG2 cells but not in LO2 cells. Conclusion: Laminarin inhibited the proliferation of Bel-7404 and HepG2 cells and inhibited the growth of tumors in Hepa 1-6 tumor-bearing mice by upregulating SMP-30.

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Senescence marker protein 30 (SMP30) serves as a potential prognostic indicator in hepatocellular carcinoma

Cited by 17 publications

References 27 publications

GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

Senescence Marker Protein 30 (SMP30): A Novel Pan-Species Diagnostic Marker for the Histopathological Diagnosis of Breast Cancer in Humans and Animals

Laminarin from Seaweed (Laminaria japonica) Inhibits Hepatocellular Carcinoma Through Upregulating Senescence Marker Protein-30

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