Senescence and adiponectin signaling — Studies in canine testis

Ramisz, Grzegorz; Turek, Wiktor; Chmurska-Gąsowska, Maria; Rak, Agnieszka; Pietsch-Fulbiszewska, Agnieszka; Gałuszka, Anna; Kotula–Balak, Małgorzata; Tarasiuk, K.

doi:10.1016/j.aanat.2020.151606

Cited by 6 publications

(2 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, in cryptorchid dogs, the right testis is more often affected than the left [38]. Our prior studies have demonstrated that senescence markers can be useful in the diagnosis of cryptorchidism in mixed-breed dogs [39]. In horses, as in dogs or humans, testis/testes located in the abdominal cavity can result in neoplasm development [40,41].…”

Section: Discussionmentioning

confidence: 99%

The G-Protein-Coupled Membrane Estrogen Receptor Is Present in Horse Cryptorchid Testes and Mediates Downstream Pathways

Witkowski

Pardyak

Pawlicki

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Cryptorchidism in horses is a commonly occurring malformation. The molecular basis of this pathology is not fully known. In addition, the origins of high intratesticular estrogen levels in horses remain obscure. In order to investigate the role of the G-protein-coupled membrane estrogen receptor (GPER) and establish histological and biochemical cryptorchid testis status, healthy and cryptorchid horse testes were subjected to scanning electron microscopy analysis, histochemical staining for total protein (with naphthol blue black; NBB), acid content (with toluidine blue O; TBO), and polysaccharide content (with periodic acid–Schiff; PAS). The expression of GPER was analyzed by immunohistochemistry and Western blot. GPER-mediated intracellular cAMP and calcium (Ca2+) signaling were measured immunoenzymatically or colorimetrically. Our data revealed changes in the distribution of polysaccharide content but not the protein and acid content in the cryptorchid testis. Polysaccharides seemed to be partially translocated from the interstitial compartment to the seminiferous tubule compartment. Moreover, the markedly decreased expression of GPER and GPER downstream molecules, cAMP and Ca2+, suggests their potential role in testis pathology. Increased estrogen levels in cryptorchid conditions may be linked to disturbed GPER signaling. We postulate that GPER is a prominent key player in testis development and function and may be used as a new biomarker of horse testis in health and disease.

show abstract

Section: Discussionmentioning

confidence: 99%

The G-Protein-Coupled Membrane Estrogen Receptor Is Present in Horse Cryptorchid Testes and Mediates Downstream Pathways

Witkowski

Pardyak

Pawlicki

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…In particular, in mammals, numerous other adipokines directly are able to influence male spermatogenic function (Milon et al 2019 ; Kotula-Balak et al 2022 ) reported that in landrace boar, immunocastration results in altered adipokine and leptin system. Moreover, some authors report that the change in adipokine expression and their receptor in testis are correlated with senescence and reproductive pathology (cryptorchidism and testis tumors) (Duliban et al 2020 ) 0; Ramisz et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Presence and localization of apelin and its cognate receptor in canine testes using immunohistochemical and RT-PCR techniques

et al. 2022

View full text Add to dashboard Cite

Apelin, a member of the adipokine family, is a novel endogenous peptide which regulates the male reproductive system of mammals by interacting with a specific receptor. Recent studies have highlighted that apelin may play a role in the regulation of reproduction by reducing testosterone production and inhibiting LH secretion. To the best of our knowledge, there is no available data on the presence of the apelin and its receptor in canine testes. Therefore, the aim of this study was to reveal the presence of apelin and evaluate its distribution in the canine testes using immunohistochemical and RT-PCR techniques. For this purpose, five fertile and healthy male dogs were subjected to elective orchiectomy. The immunohistochemical reaction revealed the presence of apelin and its receptor in the canine testes. Apelin was localized in spermatids and spermatozoa with a positive signal in the “acrosomal bodies”. As regards the apelin receptor, a positive immunoreaction was detected in the cytoplasm of the cells localized near to the basal membrane of the seminiferous tubules and in the cytoplasm of Leydig cells. The RT-PCR analysis showed the presence of transcripts for apelin and apelin receptor in all of the samples under study. A 35kDa band confirmed apelin receptor protein expression in all of the samples analysed. In conclusion, the paracrine and endocrine role of apelin and its cognate receptor on male reproduction reported in humans and laboratory animals could also be hypothesized in dogs.

show abstract

Autophagy-related 7 protein-dependent autophagy mediates resveratrol-caused upregulation of mitochondrial biogenesis and steroidogenesis in aged Leydig cell

Lin,

Zhang,

Zhu

et al. 2023

Mol Biol Rep

View full text Add to dashboard Cite

Senescence and adiponectin signaling — Studies in canine testis

Cited by 6 publications

References 65 publications

The G-Protein-Coupled Membrane Estrogen Receptor Is Present in Horse Cryptorchid Testes and Mediates Downstream Pathways

The G-Protein-Coupled Membrane Estrogen Receptor Is Present in Horse Cryptorchid Testes and Mediates Downstream Pathways

Presence and localization of apelin and its cognate receptor in canine testes using immunohistochemical and RT-PCR techniques

Autophagy-related 7 protein-dependent autophagy mediates resveratrol-caused upregulation of mitochondrial biogenesis and steroidogenesis in aged Leydig cell

Contact Info

Product

Resources

About