Sendai virus-specific, H-2-restricted cytotoxic T lymphocyte responses of nude mice grafted with allogeneic or semi-allogeneic thymus glands.

Abstract

The in vitro secondary cytotoxic T lymphocyte (CTL) response to Sendai virus-treated stimulator cells by primed spleen cells from thymus gland-grafted nude mice was examined. BALB/c (H-2d) nude mice grafted with allogeneic C57BL/10 (H-2b) thymus glands developed CTL responses directed exclusively to Sendai virus-infected H-2d target cells. (C57BL/6 X BALB/c)F1 nude mice grafted with thymus glands of either parent developed CTL responses preferentially against infected target cells expressing the MHC antigens present in the parental thymus graft, but also had detectable activity for infected target cells of the parental haplotype not expressed in the thymus. These results provide evidence against the concept that self recognition by MHC-restricted CTL is directed exclusively by the MCH type of the thymus.

Genetic control of the immune response to myoglobins. Both low and high responder T cells tolerant to the other major histocompatibility complex help high but not low responder B cells

“…This possibility is compatible with the observation of peripheral chimerization without thymic chimerization. Moreover, there is precedent for such a suggestion in the studies of cytotoxic T cells in thymus-engrafted nude mice by Zinkernagel et al (37), Lake et al (38), and Kruisbeek et al (39). It is more difficult, however, to reconcile extrathymic repertoire development with the contraction of the repertoire in F1 ~ parent bone marrow chimeras despite peripheral F1 cells (1-6, 9, 10, 40, 41), or with the restriction to the thymic parental haplotype of helper cells from ~ nude mice engrafted with a parental thymus (42) and homozygous nude mice engrafted with an allogeneic thymus (43).…”

The concurrent maturation of mouse and human thymocytes in human fetal thymus implanted in NIH-beige-nude-xid mice is associated with the reconstitution of the murine immune system.

“…In contrast to the etiology of the T cell defect in BNX mice, the cause of the T cell defect in SCID mice is not the absence of a functional thymus but rather impaired maturation of T cells secondary to the defective recombination of the antigen receptor genes (5,6). Therefore, while the immunodeficiency of congenitally athymic mice can be restored by the implantation of either allogeneic mouse thymus (7)(8)(9)(10) or xenogeneic rat thymic tissue (11), the intrinsic cellular immune defect of SCID mice is not corrected by thymic transplantation (12).…”

Use of Cultured Thymic Tissues for the Regeneration of the Thymus

“…The authors' overall conclusion was therefore tempered to acknowledging that xenogeneic transplantation of CTF was only partially successful in restoring immune function to nude mice. Much other work was conducted during this period [22][23][24] and indicated a lack of consistency. It appears that thymocytes in the thymus can select for the entire or partial population of, or none of the T-cell self-receptors [25].…”