Sendai virus induces high levels of tumor necrosis factor mRNA in human peripheral blood leukocytes

Berent, Susan L.; Torczynski, Richard M.; Bollon, Arthur P.

doi:10.1093/nar/14.22.8997

Cited by 36 publications

(23 citation statements)

References 39 publications

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“…The hypothesis we tested was that HSV infection would induce inflammatory or activated M~b to synthesize and secrete soluble cytotoxic factors such as TNF (Gifford & Lohmann-Matthes, 1986;Urban et al, 1986), which could then be selectively toxic for virus-infected M~b. There have been a few recent reports of the ability of certain viruses to induce TNF (Berent et al, 1986), and for TNF to have antiviral activity or selective toxicity for virus-infected cells (Koff &Fann, 1986;Mestian et al, 1986;Wong & Goeddel, 1986). However, we did not find significant levels of TNF in the supernatant fluids of either uninfected or infected M~.…”

Section: Discussioncontrasting

confidence: 77%

Effect of Macrophage Activation on Resistance of Mouse Peritoneal Macrophages to Infection with Herpes Simplex Virus Types 1 and 2

Sit

Tenney

Rothstein

et al. 1988

Journal of General Virology

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SUMMARYTo define the effect of heterogeneity of murine peritoneal macrophages (MS) on intrinsic resistance to herpes simplex virus (HSV) infection, several MS populations were characterized for their response to infection with HSV type 1 (HSV-1) and HSV-2. Steady-state resident MS (Res MS) were compared in parallel with MS activated with Corynebacterium parvum (now designated Propionibacterium acnes) (CP MS) and thioglycollate-elicited inflammatory MS (TG M~b). Res MS were completely nonpermissive for productive virus infection and showed no c.p.e. The intrinsic resistance of CP MS to HSV infection was similar to that of Res MS, in that the infection was non-productive for infectious virus, but CP MS showed marked c.p.e. TG MS showed semi-permissiveness, with virus yields at least 10-fold higher than those in Res MS and CP MS, and marked c.p.e. The three distinct intrinsic response patterns were maintained regardless of whether MS were derived from CD-1 or B6C3F1 mice, or whether the infecting virus was HSV-1 or HSV-2. To define the level at which M S restrict HSV replication, immunofluorescence assays for viral antigens and hybridization analyses for viral DNA were performed. All MS populations showed immediate early and early virus polypeptides. Res MS and CP MS showed no viral DNA replication, but TG MS showed moderate levels of viral DNA synthesis that paralleled the infectious virus titres produced. Investigation of the mechanism for the heterogeneous intrinsic antiviral response among the M S revealed that interferon was not involved, because antiserum to mouse ot/fl interferon did not alter the intrinsic resistance patterns. Induction of c.p.e, in M~b required live, replication-competent HSV. The involvement of tumour necrosis factor (TNF) in c.p.e, was found to be unlikely; no significant amounts of TNF were detected in the culture medium of the MS, and inclusion of anti-TNF antibody did not inhibit c.p.e. INTRODUCTION Macrophages (MS) play a highly significant role in non-specific resistance to virus infection, due in part to non-permissiveness of M~b for replication of many viruses (intrinsic resistance), and inhibition of virus replication in infected permissive cells (extrinsic resistance) (for reviews,

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Section: Discussioncontrasting

confidence: 77%

Effect of Macrophage Activation on Resistance of Mouse Peritoneal Macrophages to Infection with Herpes Simplex Virus Types 1 and 2

Sit

Tenney

Rothstein

et al. 1988

Journal of General Virology

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“…Recombinant hTNF was purified from Escherichia coli bearing a plasmid with a hTNF gene (18). The specific activity of the purified hTNF was 1 -5 x 107 cytolytic units per mg and was greater than 95% pure by NaDodSO4/PAGE analysis.…”

Section: Methodsmentioning

confidence: 99%

Antibodies against amino acids 1-15 of tumor necrosis factor block its binding to cell-surface receptor.

Socher¹,

Riemen²,

Martinez³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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Human tumor necrosis factor (hTNF) mediates a variety of biologic activities, which are dependent on the attachment of hTNF to cell-surface receptors. To identify regions of the hTNF protein involved in binding hTNF to its receptor, we prepared five synthetic peptides , hTNF-(1-31), hTNF-(65-79), hTNF-(98-111), and hTNF-(124-141)] and two hydroxylamine cleavage fragments and hTNF-(40-157)] of hTNF. The hTNF-synthetic peptides and hTNF fragments were tested in hTNF receptor binding assays and in two biologic assays: cytolysis of tumor cells and suppression of lipoprotein lipase in adipocytes. Neither the synthetic peptides nor hTNF fragments were active agonists or antagonists in these assays. The synthetic peptides were also conjugated to thyroglobulin, and peptide-specific antisera were raised. All five peptide-thyroglobulin conjugates induced antibody responses to the immunizing peptide and to hTNF. Each antiserum was tested for antagonist activity in hTNF binding assays. Only antisera raised against hTNF-(1-15) or hTNF-(1-31) and antisera against whole hTNF blocked binding. IgGs purified from these three antisera also block hTNF-induced cytolysis and lipoprotein lipase suppression. We conclude that antibodies that recognize the Nterminus of hTNF block the attachment of hTNF to its cellular receptor and inhibit the biologic effects of hTNF.Tumor necrosis factor (TNF) is a cytokine secreted by activated macrophages. Carswell et al.(1) originally showed that TNF can promote the lysis of tumor cells in vitro and in vivo. Beutler et al. (2) showed that TNF was identical to another macrophage-derived protein termed cachectin. More recently, TNF has been shown to mediate a variety of biologic effects including the following: endotoxin-induced shock (3), suppression of lipoprotein lipase (LPL) activity in preadipocytes (2), stimulation of collagenase activity and prostaglandin E2 production by synovial cells (4), enhanced proliferation of fibroblasts in vitro (5), stimulation of bone resorption (6), stimulation of interleukin 1 production (7), and induction of cachexia in nude mice (8). Human TNF (hTNF) cDNAs and genomic DNA clones have been isolated and sequenced (9,10). Using these cloned genes several investigators have expressed recombinant hTNF protein and validated the diversity of biologic activities attributed to TNF (11).TNF-specific cell-surface receptors are present on several types of cells (12,13). The binding of TNF to these receptors is believed necessary for induction of the biologic effects of TNF. Therefore, it should be possible to inhibit the biologic activities of TNF by blocking the association of TNF with its cellular receptors. We examined the deduced amino acid sequence of hTNF and selected regions of the protein that might be involved in receptor-ligand interactions. To evaluate the importance of these regions in mediating the interaction of hTNF with its receptor we used two strategies. (i) Synthetic peptides were prepared based on the amino acid sequence in these regions; and the...

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“…After 3 d, they were stained with neutral red and the absorbance measured at 540 nm. For nonadherent cells, a modification of the staining protocol was used (Berent et al, 1986). The results were recorded as median inhibitory concentrations (IC &!…”

Section: Isolation Of S Marcescens and Demonstration Of Antimycoticmentioning

confidence: 99%

Oocydin A, a chlorinated macrocyclic lactone with potent anti- oomycete activity from Serratia marcescens

et al. 1999

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A unique chlorinated macrocyclic lactone, termed oocydin A, was isolated from a strain of Serratia marcescens growing as an epiphyte on Rhyncholacis pedicillata, an aquatic plant native to the Carrao river of the VenezuelanGuyanan region of South America. The lactone has a molecular mass of 470 Da, and contains one atom of chlorine, a carboxyl group and a tetrahydrofuran ring internal to a larger macrocyclic ring. MICs of approximately 003 µg ml N1were noted for oocydin A against such phytopathogenic oomycetes as Pythium ultimum, Phytophthora parasitica, Phytophthora cinnamomi and Phytophthora citrophora. With regard to the true fungi, oocydin A had either minimal or no effect against certain Fungi Imperfecti (including several pathogens of humans), two ascomycetes and a basidiomycete. Oocydin A may have potential as an antimycotic in agricultural applications and especially for crop protection.

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Sendai virus induces high levels of tumor necrosis factor mRNA in human peripheral blood leukocytes

Cited by 36 publications

References 39 publications

Effect of Macrophage Activation on Resistance of Mouse Peritoneal Macrophages to Infection with Herpes Simplex Virus Types 1 and 2

Effect of Macrophage Activation on Resistance of Mouse Peritoneal Macrophages to Infection with Herpes Simplex Virus Types 1 and 2

Antibodies against amino acids 1-15 of tumor necrosis factor block its binding to cell-surface receptor.

Oocydin A, a chlorinated macrocyclic lactone with potent anti- oomycete activity from Serratia marcescens

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