Semisynthetic Antibiotics

Fundamentals to function: Quantitative and scalable approaches for measuring protein stability

“…The importance of stability also extends beyond disease pathology. Stable proteins have industrial applications as catalysts for antibiotic and chemotherapeutic synthesis (Bruggink and Roy, 2001;Chen et al, 2006;Kondo and Hotta, 1999;Martin and Fischer, 1983;Tatsis et al, 2017;Volpato et al, 2010), catalysts for bioremediation and green chemistry (Peixoto et al, 2011;Sheldon and Woodley, 2018), lipases and proteases for detergents (Olsen and Falholt, 1998;Vojcic et al, 2015), flavoring and digestive additives (Merz et al, 2015;Raveendran et al, 2018), nanoengineering scaffolds (Ben-Sasson et al, 2021), and degradative enzymes for cellulosic biofuels (Reetz, 2013). To be used for these purposes, it is essential that native or engineered proteins not only adopt a particular fold but also remain folded under specific, and potentially harsh, conditions while still being able to carry out their desired functions.…”