Semiquantitative Evaluation of Muscle Repair by Diffusion Tensor Imaging in Mice

Hata, Junichi; Mizuno, Sakiko; Haga, Yawara; Shimoda, Masayuki; Kanai, Yae; Chiba, Kazuhiro; Nakamura, Masaya; Horiuchi, Keisuke

doi:10.1002/jbm4.10040

Cited by 8 publications

(9 citation statements)

References 33 publications

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“…Hence, the skeletal muscle is anisotropic. When the skeletal muscle is damaged, the diffusion of water molecules inside and outside the skeletal muscle cells changes; the oedema of the interstitium affects changes in DTI parameters such as ADC and FA values, reflecting anisotropy [ 18 , 36 ]. Our study found that the ADC values increased at 0.5 h after eccentric exercises, peaked at 24–48 h, and then declined; the trend of FA values was the opposite of that of ADC.…”

Section: Discussionmentioning

confidence: 99%

“…Diffusion tensor imaging (DTI) is a technology to quantitatively evaluate diffusion anisotropy of water molecules from multiple directions, which can provide information on the organisational proliferation and direction of the fibre bundles [ 16 , 17 ]. Currently, DTI has been widely used in the field of study of the skeletal muscle and the nervous system [ 18 , 19 ]. T2 mapping can reflect specificity through the detection of transverse magnetisation attenuation of tissues.…”

Section: Introductionmentioning

confidence: 99%

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Exercise-induced muscle damage: multi-parametric MRI quantitative assessment

Lyu

Gao

Liu

et al. 2021

BMC Musculoskelet Disord

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Background To explore the value of magnetic resonance quantitative analysis using diffusion tensor imaging, T2 mapping, and intravoxel incoherent motion in the evaluation of eccentric exercise-induced muscle damage and to compare the effects of various eccentric exercise modes at different time points in rats. Methods A total of 174 Sprague-Dawley male rats were randomly divided into five groups: control, once-only exercise, continuous exercise, intermittent exercise, and once-fatigue exercise groups. Each experimental group was divided into seven time-subgroups: 0.5 h, 24 h, 48 h, 72 h, 96 h, 120 h and 168 h after exercise. The quadriceps femoris muscles were then scanned using magnetic resonance imaging. The apparent diffusion coefficient and fractional anisotropy values of diffusion tensor imaging, T2 values of T2 mapping, D and D* values of intravoxel incoherent motion and optical density values of desmin were measured. Associations among different eccentric exercise programmes, magnetic resonance imaging findings, and histopathological results were evaluated. Dunnett’s test, two-way repeated measures analysis of variance, and Pearson correlation analysis were used for statistical analysis. Results Diffusion tensor imaging showed that the number of muscle fibre bundles decreased to varying degrees with different time points and eccentric exercises. Apparent diffusion coefficient values of the exercise groups showed a trend that first increased and then decreased, the opposite of fractional anisotropy. The specimens in all eccentric exercise programmes showed high signal T2 values after exercise, the highest among which was in the once-fatigue exercise group. D and D* in the experimental groups were significantly higher than those in the control group at 0.5–48 h after exercise. The apparent diffusion coefficient, fractional anisotropy, T2, D and D* values correlated with the optical density values of desmin. Conclusions Diffusion tensor imaging, T2 mapping, and intravoxel incoherent motion technology accurately reflect the degree of skeletal muscle damage and recovery associated with eccentric exercise. The degree of muscle damage was the lowest in the continuous exercise group and the highest in the once-fatigue exercise group, which may provide more information and guidance for the formulation of physical and athletic training programmes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exercise-induced muscle damage: multi-parametric MRI quantitative assessment

Lyu

Gao

Liu

et al. 2021

BMC Musculoskelet Disord

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“…Additionally, because the skeletal muscle cell diameter is larger than the central nervous system cell diameter [41, 42], a diffusion time setting different from that of the nervous system is mandatory [33, 43]. However, during DWI of the skeletal muscles, a short diffusion time similar to that for the central nervous system is often used [10–15]. As the skeletal muscles have short T2 values and a long TE cannot be set, it is difficult to utilize a diffusion time suitable for the skeletal muscles from the viewpoint of SNR with the pulsed-field gradient spin-echo method.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, studies using diffusion-weighted imaging (DWI) for assessing muscles, especially diffusion tensor imaging (DTI) [10–14], have been reported. Various factors, such as sex [11], muscle macro-morphological structure (parallel muscle or bipennate muscle) [11], and age [15], can affect DTI values.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive technique to evaluate the muscle fiber characteristics using q-space imaging

et al. 2019

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Background Skeletal muscles include fast and slow muscle fibers. The tibialis anterior muscle (TA) is mainly composed of fast muscle fibers, whereas the soleus muscle (SOL) is mainly composed of slow muscle fibers. However, a noninvasive approach for appropriately investigating the characteristics of muscles is not available. Monitoring of skeletal muscle characteristics can help in the evaluation of the effects of strength training and diseases on skeletal muscles. Purpose The present study aimed to determine whether q-space imaging can distinguish between TA and SOL in in vivo mice. Methods In vivo magnetic resonance imaging of the right calves of mice (n = 8) was performed using a 7-Tesla magnetic resonance imaging system with a cryogenic probe. TA and SOL were assessed. q-space imaging was performed with a field of view of 10 mm × 10 mm, matrix of 48 × 48, and section thickness of 1000 μm. There were ten b-values ranging from 0 to 4244 s/mm 2 , and each b-value had diffusion encoding in three directions. Magnetic resonance imaging findings were compared with immunohistological findings. Results Full width at half maximum and Kurtosis maps of q-space imaging showed signal intensities consistent with immunohistological findings for both fast (myosin heavy chain II) and slow (myosin heavy chain I) muscle fibers. With regard to quantification, both full width at half maximum and Kurtosis could represent the immunohistological findings that the cell diameter of TA was larger than that of SOL ( P < 0.01). Conclusion q-space imaging could clearly differentiate TA from SOL using differences in cell diameters. This technique is a promising method to noninvasively estimate the fiber type ratio in skeletal muscles, and it can be further developed as an indicator of muscle characteristics.

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“…The sample temperature during the scan was controlled and maintained at 24°C, similar to that in our previous DWI reports. 20,24 The detailed protocols are presented in Table 1. Because the IVD is large (diameter: 3-4 mm thickness: 1 mm) enough for pixel resolution (100 µm × 100 µm)/slice thickness (sagittal: 1000 µm) ( Figure 1B) and relatively nonanisotropic tissue, we considered the influence of partial volume effects to be small.…”

Section: Mri Protocolmentioning

confidence: 99%

Quantitative analysis of intervertebral disc degeneration using Q‐space imaging in a rat model

Nakashima

Fujita

Hata

et al. 2020

Journal Orthopaedic Research

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The degree of intervertebral disc (IVD) degeneration is qualitatively evaluated on T2‐weighted imaging (T2WI). However, it is difficult to assess subtle changes in IVD degeneration using T2WI. Q‐space imaging (QSI) is a quantitative diffusion‐weighted imaging modality used to detect subtle changes in microenvironments. This study aimed to evaluate whether QSI can detect the inhibitory effects of the antioxidant N‐acetylcysteine (NAC) in IVD degeneration. We classified female Wistar rats into control, puncture, and NAC groups (n = 5 per group). In the puncture and NAC groups, IVDs were punctured using a needle. The antioxidant NAC, which suppresses the progression of IVD degeneration, was orally administered in the NAC group 1 week prior to puncture. The progression and inhibitory effect of NAC in IVD degeneration were assessed using magnetic resonance imaging (MRI): IVD height, T2 mapping, apparent diffusion coefficient (ADC), and QSI. MRI was performed using a 7‐Tesla system with a conventional probe (20 IVDs in each group). QSI parameters that were assessed included Kurtosis, the probability at zero displacement (ZDP), and full width at half maximum (FWHM). IVD degeneration by puncture was confirmed by histology, IVD height, T2 mapping, ADC, and all QSI parameters (P < .001); however, the inhibitory effect of NAC was confirmed only by QSI parameters (Kurtosis and ZDP: both P < .001; FWHM: P < .01). Kurtosis had the largest effect size (Kurtosis: 1.13, ZDP: 1.06, and FWHM: 1.02) when puncture and NAC groups were compared. QSI has a higher sensitivity than conventional quantitative methods for detecting the progressive change and inhibitory effect of NAC in IVD degeneration.

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Semiquantitative Evaluation of Muscle Repair by Diffusion Tensor Imaging in Mice

Cited by 8 publications

References 33 publications

Exercise-induced muscle damage: multi-parametric MRI quantitative assessment

Exercise-induced muscle damage: multi-parametric MRI quantitative assessment

Noninvasive technique to evaluate the muscle fiber characteristics using q-space imaging

Quantitative analysis of intervertebral disc degeneration using Q‐space imaging in a rat model

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