Semiochemical-based alternatives to synthetic toxicant insecticides for pollen beetle management

Mauchline, Alice; Hervé, Maxime; Cook, S. M.

doi:10.1007/s11829-017-9569-6

Cited by 21 publications

(23 citation statements)

References 111 publications

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“…Hokkanen 1991). Trap cropping strategies targeted against pollen beetles in OSR has been reviewed by Mauchline et al (2017b) and Skellern & Cook (in press). Late-flowering OSR cultivars could be used to extend the necessary growth stage differential between the main crop and an early-flowering trap crop.…”

Section: Cultivarmentioning

confidence: 99%

The potential of crop management practices to reduce pollen beetle damage in oilseed rape

Skellern

Cook

2017

Arthropod-Plant Interactions

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Section: Cultivarmentioning

confidence: 99%

The potential of crop management practices to reduce pollen beetle damage in oilseed rape

Skellern

Cook

2017

Arthropod-Plant Interactions

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“…), and subsequently colonise cruciferous (Brassicaceae) plants, where they acquire nutrients from buds and open flowers, mate, and oviposit into buds. Upon emergence from eggs, larvae feed on pollen within buds, eventually obtaining their nutrients from open flowers, followed by pupation in the soil under their host plant (see review by Mauchline et al (2018)).…”

Section: Introductionmentioning

confidence: 99%

Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus

et al. 2020

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The pollen beetle Brassicogethes aeneus is a serious pest of oilseed rape (Brassica napus) in Europe. Management of this pest has grown difficult due to B. aeneus’s development of resistance to pyrethroid insecticides, as well as the pressure to establish control strategies that minimise the impact on nontarget organisms. RNA interference represents a nucleotide sequence-based, and thus potentially species-specific, approach to agricultural pest control. The present study examined the efficacy of targeting the coatomer gene coatomer subunit alpha (αCOP), via both microinjection and dietary exposure to exogenous complementary dsRNA, on αCOP-silencing and subsequent mortality in B. aeneus. Beetles injected with dsRNA targeting αCOP (at 0.14 µg/mg) showed 88% and 100% mortality at 6 and 10 days post-injection, respectively; where by the same time after dietary exposure, 43%–89% mortality was observed in the 3 µg dsRNA/µL treatment, though the effect was concentration-dependent. Thus, the effect was significant for both delivery routes. In working towards RNA-based management of B. aeneus, future studies should include αCOP as a target of interest.

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“…Overwintered adult B. aeneus feed on pollen and nectar of blooming plants of various taxonomic families and later become monophagous on brassicaceous plants, where they obtain nutrients from reproductive buds and open flowers, followed by mating and subsequent oviposition into buds (usually 2−3 mm in length). Upon hatching from eggs, larvae feed on anthers within buds, followed by late first and early second instar larvae feeding in open flowers, the late second instar larvae eventually pupating in the soil under their host plant (reviewed in Mauchline et al 1 ).…”

Section: Introductionmentioning

confidence: 99%

RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle

et al. 2021

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Double-stranded RNAs (dsRNAs) represent a promising class of biosafe insecticidal compounds. We examined the ability to induce RNA interference (RNAi) in the pollen beetle Brassicogethes aeneus via anther feeding, and compared short-term (3 d) to chronic (17 d) feeding of various concentrations of dsRNA targeting αCOP (dsαCOP). In short-term dsαCOP feeding, only the highest concentration resulted in significant reductions in B. aeneus survival; whereas in chronic dsαCOP feeding, all three concentrations resulted in significant mortality. Chronic dsαCOP feeding also resulted in significantly greater mortality compared to short-term feeding of equivalent dsαCOP concentrations. Our results have implications for the economics and development of dsRNA spray approaches for managing crop pests, in that multiple lower-concentration dsRNA spray treatments across crop growth stages may result in greater pest management efficacy, compared to single treatments using higher dsRNA concentrations. Furthermore, our results highlight the need for research into the development of RNAi cultivars for oilseed rape protection, given the enhanced RNAi efficacy resulting from chronic, compared to short-term, dsRNA feeding in B. aeneus.

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Semiochemical-based alternatives to synthetic toxicant insecticides for pollen beetle management

Cited by 21 publications

References 111 publications

The potential of crop management practices to reduce pollen beetle damage in oilseed rape

The potential of crop management practices to reduce pollen beetle damage in oilseed rape

Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus

RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle

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