Seminal Plasma Zinc and Magnesium in Infertile Men

Papadimas, J.; Bontis, J.; Ikkos, D; Mantalenakis, S.

doi:10.3109/01485018308987576

Cited by 12 publications

(7 citation statements)

References 9 publications

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“…Membranes of these organelles contain Mg 2ϩ -and Ca 2ϩ -dependent ATPase competitively inhibited by Zn 2ϩ (Ronquist et al, 1978a,b). High positive correlation between zinc and magnesium in seminal plasma has previously been reported (Papadimas et al, 1983;Umeyama et al, 1986), and in this study similar strong intercorrelations between all three cations were found (r ϭ 0.79-0.86, P Ͻ 0.01).…”

Section: Discussionsupporting

confidence: 72%

Zinc, magnesium and calcium in human seminal fluid: relations to other semen parameters and fertility

Sörensen

Bergdahl²,

Hjøllund³

et al. 1999

Molecular Human Reproduction

118

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The effects of zinc, magnesium and calcium in seminal plasma on time-to-pregnancy (TTP) in healthy couples, on conventional semen parameters and computer-assisted semen analysis (CASA) parameters were evaluated. The localization of chelatable zinc ions in seminal plasma and spermatozoa were assessed by autometallography (AMG). Differences in chelatable zinc localization in samples with high and low total zinc were evaluated. Semen samples from 25 couples with short TTP and 25 couples with long TTP were subjected to conventional semen analysis, CASA, zinc and magnesium measurements by inductively coupled plasma mass spectrometry, and calcium by flame atomic absorption spectrometry. The cations were strongly inter-correlated, but no correlation with TTP or conventional semen parameters was found. Semen samples with high zinc concentrations exhibited statistically significant poorer motility assessed by the CASA parameters straight line velocity and linearity than samples with low zinc content. Calcium concentration also showed statistically significant differences for the same parameters, but the effect was removed by entering zinc concentration into a multiple regression model. Semen samples with high total zinc exhibited stronger staining of the seminal plasma at AMG. It is suggested that high seminal zinc concentrations have a suppressing effect on progressive motility of the spermatozoa ('quality of movement'), but not on percentage of motile spermatozoa ('quantity of movement').

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Section: Discussionsupporting

confidence: 72%

Zinc, magnesium and calcium in human seminal fluid: relations to other semen parameters and fertility

Sörensen

Bergdahl²,

Hjøllund³

et al. 1999

Molecular Human Reproduction

118

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“…A measurement of ''viscosity'' is frequently performed as recommended in the WHO Manual (1999), which defines an abnormal viscosity sample as one that can be drawn Arver and Sjoberg, 1982Fong et al, 1986Ford and Harrison, 1984Kilic et al, 1996Magnus et al, 1990Prien et al, 1990 Cl (mg/100 mL) 130 157 151.0 152 112 133 158 Gershbein and Thielen, 1988Hirsch et al, 1991Huggins and Johnson, 1933Huggins et al, 1942Jeyendran et al, 1989Kavanagh, 1985Rosecrans et al, 1987 K (mg/100 mL) 80 125 73.75 129 89.5 Bondani et al, 1973Gershbein and Thielen, 1988Girgis et al, 1980Hirsch et al, 1991Huggins et al, 1942 81.6 91 Jeyendran et al, 1989Kavanagh, 1985Mendiratta et al, 1980Nag and Chaudhuri, 1978Quinn et al, 1965; whole semen 88.0 113 50 126 154 Quinn et al, 1965;plasma Rosecrans et al, 1987Schirren, 1961Sheth and Rao, 1962aSheth and Rao, 196896 113.9 113 Skandhan et al, 1978Skandhan and Mazumdar, 1981Wood et al, 1982 Colleen et al, 1975Homonnai et al, 1978Jeyendran et al, 1989Kavanagh, 1985Papadimas et al, 1983 6.5 9.0 11.3 8.6…”

Section: Viscositymentioning

confidence: 99%

“…Abou-Shakra et al, 1989 Arver and Sjoberg, 1982Carpino and Siciliano, 1998Colleen et al, 1975Cooper et al, 199117.2 16.5 14 16 11.76 Hirsch et al, 1991Homonnai et al, 1978Jeyendran et al, 1989Kavanagh, 1985Lewis-Jones et al, 1996 8.4 14 15.0 Mandal and Bhattacharyya, 1986Mandal and Bhattacharyya, 1987aMandal and Bhattacharyya, 1990Mann and Lutwak-Mann, 1981Marmar et al, 1975; atomic absorption 18.9 13.4 9.26 19.0 16.6 Marmar et al, 1975; neutron activation Mawson and Fischer, 1956Mendiratta et al, 1980Papadimas et al, 1983Paz et al, 197714.64 14.7 69.29 10.6 19.5 Ponchietti et al, 1984Rosecrans et al, 1987Schoenfeld et al, 1979Sorensen et al, 1999Stankovic and Mikac-Devic, 197613 12.4 14.4 Stegmayr et al, 1982Umeyama et al, 1986Wood et al, 1982 Makler et al, 1981Mandal and Bhattacharyya, 1987bPolak and Daunter, 1984Velazquez et al, 1977 Purvis et al, 1986Rosecrans et al, 1987Schirren et al, 1977Schoenfeld et al, 1979225 302 136 Sheth and Rao, 1962bTauber et al, 1975…”

Section: Viscositymentioning

confidence: 99%

A Review of the Physical and Chemical Properties of Human Semen and the Formulation of a Semen Simulant

Owen

Katz

2005

Journal of Andrology

348

298

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A fluid medium was developed to simulate the salient physical and chemical properties of human semen. The composition of the medium was based upon an extensive review of the literature on constituents of human semen. In choosing the ingredients for this medium, the goal was to emphasize properties that influence interactions of human semen with topical contraceptive, prophylactic, or therapeutic products. Among these properties, pH and buffering capacity, osmolarity, ionic strength, and rheological properties play dominant roles in the physico-chemical processes that govern drug release kinetics and delivery vehicle distribution. W hen therapeutic, contraceptive, or prophylactic formulations are applied to the vagina, they encounter a variety of fluids with widely varying physical and chemical properties. These fluids include those that originate in the vagina and those that flow into it (eg, cervical mucus and semen). The fluid actually present at any location within the vagina is a mixture, to a varying extent, of these fluids. The flow, retention, drug delivery kinetics, and bioactivity of vaginal formulations depend upon their interactions with these resident fluids. Work done by our group has determined that the physical and chemical properties of both the delivery vehicle and the surrounding environment are important factors in determining product performance (Katz et al, 1998;Owen et al, 1999aOwen et al, , 2000Owen et al, , 2001Owen et al, , 2003. Understanding of these interactions can, therefore, aid in the design and development of new and improved formulations.One component of such research is the in vitro testing of formulations with fluids representative of those that will be encountered within the vagina. Our laboratory has been developing and applying in vitro assays that focus on how the deployment and delivery of contraceptive and prophylactic compounds are affected by the properties of the delivery vehicle and its interactions with the surrounding fluids. To develop these assays using standardized materials of sufficient volume, we have found it useful to employ simulants of ambient biological fluids. Our for- mulation of a vaginal fluid simulant has been described in a previous publication (Owen and Katz, 1999b), and our semen simulant (and earlier versions) was presented in a number of studies of contraceptive and microbicidal gels (Owen et al, 2003(Owen et al, , 2004Geonnotti and Katz, 2004;Geonnotti et al, 2005a;Geonnotti et al, 2005b). Here we describe the formulation of a semen simulant embodying salient physical and chemical components and properties of human semen. This is based on a comprehensive review of the literature, and we present here, as well, an updated summary of the constituents of human semen. Materials and MethodsThe quantity and composition of human semen have been studied for a variety of reasons (eg, for the diagnosis of conditions such as prostatitis, infertility, and cancer), and the results of these studies were used in the development of our simulant. It i...

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“…Some workers suggest the measurement of magnesium, rather than zinc, as markers for infection because it was found that seminal magnesium levels were markedly decreased during prostate infection. [16] In addition, reduced seminal magnesium is associated with increased thromboxane levels which may cause increase nitric oxide and vasoconstriction that results in premature ejaculation. [17] It is known that calcium is required to initiate the acrosome reaction with its attendant release of enzymes and membrane alterations needed for successful egg-sperm interaction.…”

Section: Introductionmentioning

confidence: 99%

Seminal oxidative stress markers, calcium, magnesium, and semen profile of infertile diabetic and nondiabetic Nigerian men

Bassey

Isong

Esiere

et al. 2019

Int J App Basic Med Res

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Context: Oxidative stress, vitamin, and macroelement deficiencies have been implicated in male infertility. It is unknown if diabetes mellitus with its attendant increased oxidative stress makes the seminal quality of the diabetic infertile men worse compared to their nondiabetic counterparts. Aims: The study investigated semen parameters, seminal plasma calcium, magnesium, Vitamins C and E, and total antioxidant capacity (TAC) in diabetic and nondiabetic infertile men. Settings and Design: This was a cross-sectional study involving 30 infertile men with type 2 diabetes, 30 infertile nondiabetic men and 30 fertile men. Subjects and Methods: Fasting plasma glucose, glycated hemoglobin, seminal plasma calcium, magnesium, TAC, Vitamin C, Vitamin E, semen analysis, and cultures carried out using the standard procedures. Statistical Analysis Used: Data were analyzed by the analysis of variance and Student's t -test; the level of significance was set at P < 0.05. Results: Both infertile groups had significantly lower ( P < 0.0001) sperm count, percentage motility, TAC, Vitamin E and C, magnesium and calcium when compared to the fertile group. However, there was no statistically significant difference ( P > 0.05) in the mean values of these parameters among the two infertile groups. The infertile men had a significantly higher ( P = 0.034) frequency of bacterial isolates compared to the fertile men. Staphylococcus aureus was the most frequent organism isolated. Conclusions: Seminal calcium, magnesium, TAC, and Vitamin E and C were lower in both infertile diabetic and nondiabetic men as compared to that of fertile men, but the levels of these analytes were comparable in the infertile diabetic and nondiabetic men.

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Seminal Plasma Zinc and Magnesium in Infertile Men

Cited by 12 publications

References 9 publications

Zinc, magnesium and calcium in human seminal fluid: relations to other semen parameters and fertility

Zinc, magnesium and calcium in human seminal fluid: relations to other semen parameters and fertility

A Review of the Physical and Chemical Properties of Human Semen and the Formulation of a Semen Simulant

Seminal oxidative stress markers, calcium, magnesium, and semen profile of infertile diabetic and nondiabetic Nigerian men

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