Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation

Murdica, Valentina; Giacomini, Elisa; Alteri, Alessandra; Bartolacci, Alessandro; Cermisoni, Greta Chiara; Zarovni, Nataša; Papaleo, Enrico; Montorsi, Francesco; Salonia, Andrea; Viganò, Paola; Vago, Riccardo

doi:10.1016/j.fertnstert.2019.01.030

Cited by 77 publications

(86 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A total of 1474 proteins were identified in all seminal exosomes samples. Enrichment analysis of GO terms revealed that, in term of cellular components, exosome-associated proteins were mostly linked to exosomes and cytoplasm and the main biological processes included metabolism, energy pathways, protein metabolism, cell growth and maintenance and transport, which is consistent with previous studies [ 34 , 69 , 86 , 87 , 102 ]. The proteins identified included exosome-marker proteins, such as HSP70, CD81, apoptosis-linked gene 2-interacting protein X (ALIX), ACTB, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycerate dehydrogenase (PHGDH) and galectin-3-binding protein (LGALS3BP).…”

Section: Proteomic Landscape Of Sp: a Potential Indicator Of Spermsupporting

confidence: 90%

“…In addition to soluble molecules and extracellular proteins, a substantial number of proteins identified in SP are cytoplasmic, associated with the great number of seminal exosomes present in this fluid. These vesicles can be collected from ejaculates by centrifugation, to remove the SPZ, followed by different ultracentrifugation steps of the supernatant [ 6 , 23 , 34 , 68 , 86 ]. This permits an analysis of seminal proteome associated exclusively with these extracellular vesicles.…”

Section: Proteomic Landscape Of Sp: a Potential Indicator Of Spermmentioning

confidence: 99%

“…The regulation of all these sperm functions surely depends on the ability of exosomes to bind to SPZ membrane and fuse with them; several conditions—such as pH, temperature and zinc concentrations—have been shown to influence the exosomal uptake by SPZ [ 31 , 32 , 33 ]. However, SPZ still remain receptive to exosome-shuttled cargos after ejaculation, benefiting from the exosomal transfer of key proteins, in terms of motility, capacitation and quality [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…In this regard, although exosomes isolated from SP of normo-, astheno- and azoospermic patients display similar features in terms of shape, size and expression of typical exosomal markers, they differ in their cargo. In fact, just normozoospermic-derived exosomes are able to improve SPZ motility and trigger capacitation [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

Candenas

Chianese

2020

IJMS

View full text Add to dashboard Cite

Infertility has become a global health issue, with approximately 50% of infertility cases generated by disorders in male reproduction. Spermatozoa are conveyed towards female genital tracts in a safe surrounding provided by the seminal plasma. Interestingly, this dynamically changing medium is a rich source of proteins, essential not only for sperm transport, but also for its protection and maturation. Most of the seminal proteins are acquired by spermatozoa in transit through exosomes (epididymosomes and prostasomes). The high number of seminal proteins, the increasing knowledge of their origins and biological functions and their differential expression in the case of azoospermia, asthenozoospermia, oligozoospermia and teratozoospermia or other conditions of male infertility have allowed the identification of a wide variety of biomarker candidates and their involvement in biological pathways, thus to strongly suggest that the proteomic landscape of seminal plasma may be a potential indicator of sperm dysfunction. This review summarizes the current knowledge in seminal plasma proteomics and its potentiality as a diagnostic tool in different degrees of male infertility.

show abstract

Section: Proteomic Landscape Of Sp: a Potential Indicator Of Spermsupporting

confidence: 90%

Section: Proteomic Landscape Of Sp: a Potential Indicator Of Spermmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

Candenas

Chianese

2020

IJMS

View full text Add to dashboard Cite

show abstract

“…(c)Fraser et al (2003). (d)Fraser et al (2006),Levin (2005), andMurdica et al, 2019. (e) Austin (1951),Austin and Bishop (1952),Bailey (2010),Cohen-Dayag et al (1995).…”

mentioning

confidence: 99%

The Clitoris—An Appraisal of its Reproductive Function During the Fertile Years: Why Was It, and Still Is, Overlooked in Accounts of Female Sexual Arousal

Levin

2019

Clinical Anatomy

View full text Add to dashboard Cite

Stimulating the clitoris activates the brain to instigate changes in the female genital tract, namely, the enhancement of vaginal blood flow that increases vaginal luminal pO2, vaginal transudate (lubrication) facilitating painless penile penetration and partial neutralization of the basal luminal acidic pH, vaginal tenting, and ballooning delaying sperm transport and allowing semen de‐coagulation and capacitation (sperm activation) factors to act until arousal ends (often by orgasm induction). All these genital changes taken together are of major importance in facilitating the possibility of reproductive success (and thus gene propagation) no matter how or when the clitoris is stimulated—they reveal its overlooked reproductive function. Of course, also commensurate with these changes, is its activation of sexual pleasure. The clitoris thus has both procreative (reproductive) and recreative (pleasure) functions of equal importance. Clitoridectomy creates not only sexual disability but also a reproductive disability. Clin. Anat. 32:136–145, 2019. © 2019 Wiley Periodicals, Inc.

show abstract

The Knowns and Unknowns about Epididymal Extracellular Vesicles in Different Animal Species

Rowlison

Comizzoli

2021

Advanced Biology

View full text Add to dashboard Cite

Sperm maturation during epididymal transit is a long and complex process. Although the roles of epididymal extracellular vesicles (EVs) on sperm quality have been extensively studied in recent years, there are still a lot of unexplored areas and too few species that are studied. The objective of this review is to focus on the contribution of epididymal EVs through the apocrine secretion of key factors, including proteins and small RNAs. Furthermore, the authors explore the alterations in the content of these vesicles related to male fertility and the effects of environmental stressors, and how these factors vary across taxa. Last, potential applications are covered, and the next steps in that field of research are highlighted.

show abstract

Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation

Cited by 77 publications

References 44 publications

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

The Clitoris—An Appraisal of its Reproductive Function During the Fertile Years: Why Was It, and Still Is, Overlooked in Accounts of Female Sexual Arousal

The Knowns and Unknowns about Epididymal Extracellular Vesicles in Different Animal Species

Contact Info

Product

Resources

About