Seminal plasma hormonal profile of Arabian stallions that are classified ‘good’ or ‘poor’ for semen freezing

El-Badry, Diya A.; El‐Maaty, Amal M. Abo

doi:10.1016/j.apjr.2016.10.012

Cited by 10 publications

(10 citation statements)

References 45 publications

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“…Although SP activates the motility of epididymal spermatozoa [23], it seems that this physiological function does not always affect ejaculated spermatozoa in the same way when SP is added [8,10,12,15]. The most likely reason for this is the variability in the SP components among individual stallions [9], and particularly, between good and poor freezers [18]. Moreover, the motility stimulant aminopeptidase N differs between the seminal plasma of good and poor freezers [15].…”

Section: Discussionmentioning

confidence: 99%

“…Hence, the development of spermatozoa is complete before the semen is manipulated for freezing. As the seminal plasma components differ between good and poor freezers [18], we can assume that collected ejaculate from poor freezers has predetermined freezability. This is further supported by a study by Cabrera et al [32], who found different lipidomic profiles in poor compared to good freezers and linked this change to freezability.…”

Section: Discussionmentioning

confidence: 99%

“…These inconsistent results are probably related to the different methods of semen processing used (with vs. without the selection of the best spermatozoa), the origin of the SP (heterologous vs. homologous; pooled vs. unpooled), the concentration of SP (5%, 20%, or 30% v / v ), and the time of SP addition (before cryoconservation vs. post-thaw). The origin of seminal plasma seems to be one of the most important factors, as differences in SP composition between individual stallions [16] and good or poor freezers were found [17,18]. Although the addition of SP before storage was shown to increase hydrogen peroxide production and sperm chromatin damage in cooled semen [19], the SP was mostly added before cryopreservation [8,10,12,15].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing

Šichtař

Bubeníčková

Sirohi

et al. 2019

Animals

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The aim of this study was to evaluate the effect of the addition of two types of seminal plasma (SP) after thawing on the functional characteristics of frozen–thawed (F–T) spermatozoa of poor freezing stallions during prolonged incubation periods. Seminal plasma from stallions with 35–40% (standard seminal plasma, (S-SP)) and 60–70% (above standard seminal plasma, (A-SP)) progressively motile spermatozoa after thawing was used. The motility, kinematic parameters (Computer Assisted Sperm Analysis), distribution of spermatozoa into subpopulations, integrity (carboxyfluorescein diacetate/propidium iodide staining), and functionality (hypo-osmotic swelling (HOS) test) of the spermatozoa plasma membrane were evaluated after thawing (T0) and after 30 min (T30) of incubation at 37 °C. There was no effect of SP addition on spermatozoa motility, but there was a significant positive effect on the kinematic parameters at T0 and T30. The addition of SP significantly increased the percentage of spermatozoa in the fast subpopulation at T0 as well as at T30. Plasma membrane integrity was not affected by the treatment, but functionality significantly decreased by 5% compared to the control group when samples were incubated for 30 min with A-SP. In conclusion, generally, the post-thaw addition of seminal plasma positively affected the post-thaw quality of semen from poor freezing stallions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing

Šichtař

Bubeníčková

Sirohi

et al. 2019

Animals

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“…Several researchers have added various substances to semen extenders, such as milk, caseinate, and lactoferrin [120]. Other researchers added hormones, such as insulin [171], follicle-stimulating hormone [172], and testosterone [173], to the semen extenders. The addition of selenium improved male reproductive performance by potentiating semen quality and suppressing free radicals [174,175].…”

Section: Other Additionsmentioning

confidence: 99%

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Bustani

Baiee

2021

Vet World

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Reproduction is fundamental for all living things as it ensures the continued existence of a species and an improved economy in animal husbandry. Reproduction has developed since history, and diverse processes, such as artificial insemination and in vitro fertilization, have been developed. Semen extenders were discovered and developed to protect sperm from harmful factors, such as freeze and osmotic shock, oxidative stress, and cell injury by ice crystals. Semen extenders preserve sperm by stabilizing its properties, including sperm morphology, motility, and viability and membrane, acrosomal, and DNA integrity. Therefore, semen extenders must provide a favorable pH, adenosine triphosphate, anti-cooling and anti-freeze shock, and antioxidant activity to improve semen quality for fertilization. Hence, this review provides precise data on different semen extenders, preservative mechanisms, and essential additives for semen extenders in different animals.

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“…Stallions can be classified as “good or bad freezers” depending on the freezability of their ejaculates (El‐Badry et al., 2016), and individual stallion variability in semen quality has become one of the main challenges for the efficiency of sperm cryopreservation (Yeste et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

Relationship between sperm ubiquitination and equine semen freezability

Lançoni

Celeghini

Gonella-Diaza

et al. 2022

Reprod Domestic Animals

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This study aimed to assess the semen ubiquitin levels of stallions with good (GF) and poor semen freezability (PF) and to evaluate the relationship between sperm ubiquitination and sperm morphological defects. Five ejaculates from eight adult stallions (n = 40) were collected and cryopreserved. Then, the ubiquitin level in equine sperm cells was assessed by immunohistochemistry with epifluorescence microscopy, and sperm morphology was assessed by differential interference contrast microscopy. Sperm cells were classified according to the intensity (classification 1: from I to IV; I = very low ubiquitin intensity and IV = very high ubiquitin intensity) and location of ubiquitin staining (classification 2). Statistical analyses were performed using SAS software (version 9.4), and p ≤ .05 was considered significant. We observed that PF stallions showed higher percentages (p < .05) of sperm cells with high ubiquitination (11.82% of ubiquitin intensity grade I, 39.13% of ubiquitin intensity grade II, 27.25% of ubiquitin intensity grade III, and 20.67% of grade IV), while GF stallions showed higher percentages (p < .05) of sperm cells with lower staining intensity (28.52% grade I, 59.83% grade II, 7.92% grade III, and 7.02% grade IV). Furthermore, for PF stallions, 23 significant correlations were detected (p < .05) between sperm abnormalities and ubiquitin intensity in different sperm regions. Increased ubiquitination of the sperm head, midpiece, and tail was positively correlated with their respective morphological defects. We concluded that high sperm ubiquitin levels are observed in ejaculates from stallions with poor semen quality (poor freezability), and ubiquitin marking in specific cellular locations can identify sperm morphological defects.

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Seminal plasma hormonal profile of Arabian stallions that are classified ‘good’ or ‘poor’ for semen freezing

Cited by 10 publications

References 45 publications

How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing

How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Relationship between sperm ubiquitination and equine semen freezability

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