Seminal plasma biochemical markers and their association with semen analysis findings

Diamandis, Eleftherios P.; Arnett, William P; Foussias, George; Pappas, Helen; Ghandi, Shruti; Melegos, Dimitrios N.; Mullen, Brendan; Yu, He; Srigley, John R.; Jarvi, Keith

doi:10.1016/s0090-4295(98)00550-0

Cited by 67 publications

(55 citation statements)

References 38 publications

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“…This suggests a local function of PGC in ejaculated sperm, probably in the prevention of immune female responses [112]. Diamandis et al [111] also found a correlation between seminal PGC levels, sperm motility, and glucose concentrations.…”

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 90%

“…Although there are no reports of the presence of this protein in sperm, a few studies have identified PGC in human seminal plasma, originating from the prostate and seminal vesicles [111,112]. The function of PGC in semen was not yet elucidate, but it seems that the inactive PGC is converted into the active pepsin protein at the acidic pH of the vagina (below 5.0).…”

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 99%

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Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Intasqui

Camargo

Giudice

et al. 2013

J Assist Reprod Genet

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Purpose Sperm DNA fragmentation has been suggested as a marker for infertility diagnosis and prognosis. Hence, understanding its impact on male physiology and post-genomic pathways would be clinically important. We performed the proteomics and functional enrichment analyses of viable spermatozoa from ejaculates with low and high sperm DNA fragmentation to identify protein expression and pathways altered in association with sperm DNA fragmentation. Methods Sperm DNA fragmentation using the Comet assay and the Komet 6.0.1 software was assessed in raw samples from 89 subjects from a human reproduction service. The Low and High sperm DNA fragmentation groups were formed according to the Olive Tail Moment variable. Spermatozoa proteins from these groups were pooled and analyzed by a shotgun proteomic approach (2D nanoUPLC-ESI-MS E ). Differentially expressed proteins were used for a functional enrichment study. Results Two hundred and fifty-seven proteins were identified or quantified in sperm from the Low and High sperm DNA fragmentation groups. Of these, seventy-one proteins were exclusively or overexpressed in the Low group, whereas twenty-three proteins were exclusively or overexpressed in the High group. One hundred and sixty-three proteins were conserved between these groups. We also functionally related the differentially expressed proteins in viable spermatozoa from the groups. Processes such as triacylglycerol metabolism, energy production, protein folding, response to unfolded proteins, and cellular detoxification were found to be altered in these cells. Conclusions Sperm DNA fragmentation is associated with differential protein expression in viable spermatozoa. These proteins may potentially be used as biomarkers for sperm DNA integrity.

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Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 90%

Section: Enriched Functions In the Low Sperm Dna Fragmentation Groupmentioning

confidence: 99%

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Intasqui

Camargo

Giudice

et al. 2013

J Assist Reprod Genet

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show abstract

“…[8][9][10][11][12][13][14][15][16][17][18][19][20] A number of authors report on the use of inhibin B serum levels to determine testicular function. 8,[11][12][13][14]16,17,21 While inhibin B levels are generally lower in those men with more severe testicular dysfunction and is undetectable in those with a Sertoli cell only pattern on testis biopsy, inhibin B levels in men with maturation arrest or hyopospermatogenesis patterns on testis biopsies may be identical to those found in men with full sper- The workup of azoospermic males matogenesis.…”

Section: Differentiating the Causes Of Normal Volume Azoospermiamentioning

confidence: 99%

CUA Guideline: The workup of azoospermic males

Jarvi¹,

Lo²,

Fischer³

et al. 2013

CUAJ

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“…Blood plasma levels of inhibin B or follicle-stimulating hormone were proposed for NOA diagnosis, but these molecules had poor specificity and sensitivity (7,8). Protein levels in local fluids, such as seminal plasma, may have a better potential for genitourinary diagnostics (9). For example, PTGDS protein has been recently proposed as a seminal plasma biomarker for the diagnosis of OA (10).…”

mentioning

confidence: 99%

Verification of Male Infertility Biomarkers in Seminal Plasma by Multiplex Selected Reaction Monitoring Assay

Drabovich

Jarvi

Diamandis

2011

Molecular & Cellular Proteomics

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102

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Seminal plasma is a promising biological fluid to use for noninvasive clinical diagnostics of male reproductive system disorders. To verify a list of prospective male infertility biomarkers, we developed a multiplex selected reaction monitoring assay and measured the relative abundance of 31 proteins in 30 seminal plasma samples from normal, nonobstructive azoospermia and post-vasectomy individuals. Median levels of some proteins were decreased by more than 100-fold in nonobstructive azoospermia or post-vasectomy samples, in comparison with normal samples. To follow up the most promising candidates and measure their concentrations in seminal plasma, heavy isotope-labeled internal standards were synthesized and used to reanalyze 20 proteins in the same set of samples. Concentrations of candidate proteins in normal seminal plasma were found in the range 0.1-1000 g/ml but were significantly decreased in nonobstructive azoospermia and post-vasectomy. These data allowed us to select, for the first time, biomarkers to discriminate between normal, nonobstructive azoospermia, and post-vasectomy (simulated obstructive azoospermia) seminal plasma samples. Some testis-specific proteins (LDHC, TEX101, and SPAG11B) performed with absolute or nearly absolute specificities and sensitivities. Cell-specific classification of protein expression indicated that Sertoli or germ cell dysfunction, but not Leydig cell dysfunction, was observed in nonobstructive azoospermia seminal plasma. The proposed panel of biomarkers, pending further validation, could lead to a clinical assay that can eliminate the need for testicular biopsy to diagnose the category of male infertility, thus providing significant benefits to patients as well as decreased costs associated with the differential diagnosis of azoospermia. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.004127, 1-13, 2011.Human infertility affects ϳ15% of couples, with the male contributing to the infertility in 50% of all cases (1, 2). One of the most severe forms of male infertility is azoospermia, which is characterized by an absence of sperm in the semen (3). Azoospermia is diagnosed in 20% of subfertile men and has two forms: obstructive azoospermia (OA) 1 and nonobstructive azoospermia (NOA). OA is caused by a physical obstruction in the male reproductive tract. The biological outcome of OA is thus identical to that of vasectomy, which is a surgical severance of the vas deferens. NOA is a more complicated infertility syndrome with the azoospermia being secondary to a failure to produce sperm; NOA may be further subclassified as maturation arrest, Sertoli cell-only syndrome, and hypospermatogenesis (4).For most men with azoospermia, testicular biopsy is the only currently used method to definitively distinguish between OA and NOA (5, 6). Thus, there is an urgent need for an alternative noninvasive approach with better diagnostic potential. The differential diagnosis of normal, NOA, and OA (or post-vasectomy (PV)) men is required for the following reasons: (i) in infertile pati...

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Seminal plasma biochemical markers and their association with semen analysis findings

Cited by 67 publications

References 38 publications

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

CUA Guideline: The workup of azoospermic males

Verification of Male Infertility Biomarkers in Seminal Plasma by Multiplex Selected Reaction Monitoring Assay

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