Seminal peroxidase positive cells are not an adequate indicator of asymptomatic urethral genital infection

Barratt, Christopher L.R.; Robinson, Angela; Spencer, R. C.; Kinghorn, G R; White, A. Clinton; Harrison, Paul E.; Kessopoulou, Efrossini; Cooke, I. D.

doi:10.1111/j.1365-2605.1990.tb01044.x

Cited by 18 publications

(2 citation statements)

References 30 publications

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“…The latter could be explained by the presence of other genitourinary infections that were not screened for in this study. The lack of concordance between leukocyte numbers and infection has been observed by other authors (Weidner et al, 1996) and there is often a poor link between infection and the number of leukocytes in an ejaculate (Barratt et al, 1990).…”

Section: Discussionmentioning

confidence: 75%

Semen Quality of Men With Asymptomatic Chlamydial Infection

Hosseinzadeh

Eley

Pacey

2004

Journal of Andrology

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We have shown previously that the in vitro exposure of spermatozoa to elementary bodies (EBs) of Chlamydia trachomatis can lead to sperm death over a number of hours of incubation. As such, we have hypothesized that the ejaculates of men with a chlamydial infection could contain increased numbers of nonmotile (dead) spermatozoa if they are exposed to EBs prior to ejaculation. To test this hypothesis, the ejaculates of 642 men undergoing diagnostic semen analysis as part of ongoing infertility investigations with their partner were examined. All men were without symptoms of genitourinary infections and semen analysis was performed according to World Health Organisation (WHO) 1999 methods after a 3-5 day abstinence period. In addition to semen analysis, nested plasmid polymerase chain reaction (PCR) was undertaken on the ejaculate to detect the presence of C trachomatis DNA. A total of 31 semen specimens (4.9%) were found to be positive, and in 28 of these, the diagnosis was confirmed using the ligase chain reaction (LCR). Men whose ejaculates were PCR positive for chlamydial DNA had a significantly (P <.05) higher mean concentration of leukocytes (1.71 +/- 2.20 x 10(6) per mL) and a higher mean ejaculate volume (3.45 +/- 1.52 mL) than in those whose ejaculates were PCR negative (leukocyte concentration: 0.67 +/- 2.59 x 10(6) per mL; volume 2.93 +/- 1.38 mL). Leukocytospermia was twice as common in men that were PCR positive for chlamydial DNA (P <.05) but it was not always associated with the presence of chlamydial DNA in semen. However, there was no difference in the mean percent motility between the 2 groups and the proportion of asthenozoospermia also did not differ. Because these results do not confirm the hypothesis proposed from our in vitro experiments, further work needs to be undertaken to understand whether human spermatozoa are actually exposed to elementary bodies of C trachomatis in an infected individual prior to ejaculation.

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Section: Discussionmentioning

confidence: 75%

Semen Quality of Men With Asymptomatic Chlamydial Infection

Hosseinzadeh

Eley

Pacey

2004

Journal of Andrology

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“…However, these authors did not provide sufficient detail of the methods used to identify and quantify leucocytes. Identification of seminal leucocytes with standard cytochemical techniques such as the peroxidase method has been proved inadequate and inaccurate as it identifies only leucocytes with an intact peroxidase enzyme (mainly neutrophils) (Barratt et al, 1990b).…”

Section: Discussionmentioning

confidence: 99%

Origin of reactive oxygen species in human semen: spermatozoa or leucocytes?

Kessopoulou¹,

Tomlinson²,

Barratt³

et al. 1992

Reproduction

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171

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Peroxidative damage induced by reactive oxygen species (ROS) has been proposed as one of the major causes of defective sperm function. In previous studies of the production of ROS in semen, the contribution of contaminating leucocytes was not assessed. We determined the levels of ROS in 60 semen samples from men attending our infertility clinic and demonstrated by performing extraction experiments with antibody-coated magnetic beads that, within this unselected population of patients, leucocytes were the major source of ROS in the low-density Percoll fraction. Of the sperm motion parameters examined using computerized semen analysis, beat-cross frequency was the only one significantly affected by the ROS in semen.

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Infection and Male Infertility

Farid¹,

Hargreave²

1994

Male Infertility

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Seminal peroxidase positive cells are not an adequate indicator of asymptomatic urethral genital infection

Cited by 18 publications

References 30 publications

Semen Quality of Men With Asymptomatic Chlamydial Infection

Semen Quality of Men With Asymptomatic Chlamydial Infection

Origin of reactive oxygen species in human semen: spermatozoa or leucocytes?

Infection and Male Infertility

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