Seminal oxidation–reduction potential and sperm DNA fragmentation index increase among infertile men with varicocele

Tanaka, Takashi; Kobori, Yoshitomo; Terai, Kazutaka; Inoue, Y.; Osaka, Akiyoshi; Yoshikawa, Naoki; Shimomura, Yoshiharu; Suzuki, Keisuke; Minami, Tetsuji; Iwahata, Toshiyuki; Onota, Shin; Yamamoto, Atsushi; Sugimoto, Kouhei; Okada, Hiroshi

doi:10.1080/14647273.2020.1712747

Cited by 22 publications

(28 citation statements)

References 24 publications

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“…Elevated serum FSH level, higher oxidative reduction potential (ORP) and DFI values are seen in infertile men with varicocele compared to fertile controls. Furthermore, ORP and DFI was inversely related with semen parameters (Tanaka et al, 2020). Patients with grade 2/3 and normal conventional semen parameters (grade C recommendation) and patients with grade 1 varicocele and borderline/abnormal conventional semen parameter results (grade C recommendation) might be candidates for SDF testing (Esteves, 2019;Roaque and Esteves).…”

Section: Varicocelementioning

confidence: 97%

TUNEL assay—Standardized method for testing sperm DNA fragmentation

et al. 2020

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Sperm DNA integrity is important for normal functions such as fertilization, implantation, pregnancy and fetal development. Sperm DNA fragmentation (SDF) is more common in infertile men and may be responsible for poor reproductive function. Although there are a number of tests available to measure SDF, the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling TUNEL) assay using flow cytometry is becoming more popular to measure the sperm DNA fragmentation. It is a direct test that measures both single-and double-DNA strand breaks. In this review, we describe the protocol, quality control and measurement of sperm DNA fragmentation using a benchtop flow cytometer. We also briefly discuss the factors that can affect the results, challenges and clinical implications of TUNEL in assessing male infertility.

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Section: Varicocelementioning

confidence: 97%

TUNEL assay—Standardized method for testing sperm DNA fragmentation

et al. 2020

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“…Studies have shown that men with varicocele have significantly higher levels of SDF than controls regardless of their fertility status, suggesting that varicocele is independently associated with impaired DNA integrity [168][169][170][171][172][173][174][175]. A recent cross-sectional study was carried out on 2,399 men attending a fertility clinic, 16.3% (391/2,399) of whom were diagnosed with varicocele [171].…”

Section: Varicocelementioning

confidence: 99%

“…SDF testing can serve as an ancillary test to conventional semen analysis in specific clinical scenarios. Evidence indicates that higher levels of SDF are observed in patients who are unable to conceive naturally [149,[273][274][275], who present with UMI/idiopathic infertility [185,188,[276][277][278][279][280][281][282], have RPL [116,138,[180][181][182][183][283][284][285][286][287][288][289][290][291][292][293][294][295], are diagnosed with varicocele [169][170][171][172][173][174][296][297][298][299][300][301][302][303][304]…”

Section: Strengthsmentioning

confidence: 99%

“…SDF testing can serve as an ancillary test to conventional semen analysis in specific clinical scenarios. Evidence indicates that higher levels of SDF are observed in patients who are unable to conceive naturally [ 149 273 274 275 ], who present with UMI/idiopathic infertility [ 185 188 276 277 278 279 280 281 282 ], have RPL [ 116 138 180 181 182 183 283 284 285 286 287 288 289 290 291 292 293 294 295 ], are diagnosed with varicocele [ 169 170 171 172 173 174 296 297 298 299 300 301 302 303 304 305 ], have a negative ART outcome [ 13 41 88 117 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 …”

Section: Strengths-weaknesses-opportunities-threats (Swot) Analysis Omentioning

confidence: 99%

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Sperm DNA Fragmentation: A New Guideline for Clinicians

Agarwal

Majzoub

Baskaran

et al. 2020

World J Mens Health

175

144

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Sperm DNA integrity is crucial for fertilization and development of healthy offspring. The spermatozoon undergoes extensive molecular remodeling of its nucleus during later phases of spermatogenesis, which imparts compaction and protects the genetic content. Testicular (defective maturation and abortive apoptosis) and post-testicular (oxidative stress) mechanisms are implicated in the etiology of sperm DNA fragmentation (SDF), which affects both natural and assisted reproduction. Several clinical and environmental factors are known to negatively impact sperm DNA integrity. An increasing number of reports emphasizes the direct relationship between sperm DNA damage and male infertility. Currently, several assays are available to assess sperm DNA damage, however, routine assessment of SDF in clinical practice is not recommended by professional organizations. This article provides an overview of SDF types, origin and comparative analysis of various SDF assays while primarily focusing on the clinical indications of SDF testing. Importantly, we report four clinical cases where SDF testing had played a significant role in improving fertility outcome. In light of these clinical case reports and recent scientific evidence, this review provides expert recommendations on SDF testing and examines the advantages and drawbacks of the clinical utility of SDF testing using Strength-Weaknesses-Opportunities-Threats (SWOT) analysis.

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“…SDF can also be produced by intracellular ROS [60]. Further, there are seemingly contradictory results where some authors observed a positive correlation using a sperm chromatin dispersion test [61][62][63] but another did not find it using a sperm chromatin structure assay [34]. The disparity of techniques used can become biased when comparing results.…”

Section: Sperm Parameters Relationshipmentioning

confidence: 99%

Relationship of Seminal Oxidation-Reduction Potential with Sperm DNA Integrity and pH in Idiopathic Infertile Patients

Garcia-Segura

Ribas-Maynou

Lara-Cerrillo³

et al. 2020

Biology

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Seminal oxidative stress (OS) is one of the most promising factors to describe the causes of idiopathic male infertility. Redox balance is essential in several biological processes related to fertility, so alterations such as high reactive oxygen species (ROS) levels or low antioxidant agent levels can compromise it. MiOXSYS has been developed to evaluate the seminal static oxidation-reduction potential (sORP) and it has been proposed as an effective diagnostic biomarker. However, its relationship with parameters like sperm DNA fragmentation (SDF), chromatin compaction status or seminal pH requires further analysis, making it the object of this study. Semen and sORP analysis were performed for all samples. A terminal deoxynucleotidyl transferase dUTP nick end labeling assay (TUNEL) and Comet assay were used to assess SDF and chromomycin a3 (CMA3) test to assess sperm chromatin compaction. Regarding sORP measures, it was found that alkaline pH has an effect on sample reproducibility. To our knowledge, this unexpected effect has not been previously described. A statistical analysis showed that sORP correlated negatively with CMA3 positive cells and sperm motility, but not with SDF. As redox dysregulation, which occurs mainly at the testicular and epididymal level, causes chromatin compaction problems and leaves DNA exposed to damage, an excess of ROS could be counterbalanced further by a seminal supply of antioxidant molecules, explaining the negative correlation with CMA3 positive cells but no correlation with SDF. Our results show that the study of idiopathic infertility would benefit from a combined approach comprising OS analysis, SDF and chromatin compaction analysis.

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Seminal oxidation–reduction potential and sperm DNA fragmentation index increase among infertile men with varicocele

Cited by 22 publications

References 24 publications

TUNEL assay—Standardized method for testing sperm DNA fragmentation

TUNEL assay—Standardized method for testing sperm DNA fragmentation

Sperm DNA Fragmentation: A New Guideline for Clinicians

Relationship of Seminal Oxidation-Reduction Potential with Sperm DNA Integrity and pH in Idiopathic Infertile Patients

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