Seminal inflammasome activity in the adult varicocele

Camargo, Mariana; Ibrahim, Emad; Intasqui, Paula; Belardin, Larissa Berloffa; Antoniassi, Mariana Pereira; Lynne, Charles M.; Brackett, Nancy L.; Bertolla, Ricardo Pimenta

doi:10.1080/14647273.2020.1870756

Cited by 13 publications

(22 citation statements)

References 55 publications

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“…All three parameters were significantly reduced in men with varicocele when they underwent varicocelectomy surgery. They did not observe any significant difference in seminal ASC [ 27 ]. This may lead us to consider VCL as a typical inflammatory condition.…”

Section: Methodsmentioning

confidence: 99%

The NLRP3 inflammasome: molecular activation and regulation in spermatogenesis and male infertility; a systematic review

Tavalaee

Rahmani

Drevet

et al. 2022

Basic Clin. Androl.

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Background Infertility related to varicocele, infections, metabolic dysfunctions, oxidative stress and environmental toxicants is also associated with inflammatory processes that ultimately lead to the activation of the inflammasome pathway (IP). IP is classically activated by DAMPs, MAMPs or LAMPs, which stand for Damage-, Microbe- or Lifestyle-Associated Molecular Patterns, respectively. The most important player in IP activation is the NLRP3 (NOD[Nuclear oligomerization domain]-, LRR[Leucine rich repeat]- and pyrin domain-containing protein 3) which functions as an intracellular sensor of D/M/L-AMPs resulting in activation of caspase-1, promotion of apoptosis, pyroptosis and generation of inflammatory cytokines. This review addresses the question of whether IP activation might be associated with male infertility situations. Results & conclusions We conducted a systematic review of articles published in the Google Scholar, and PubMed databases through October 2021. It turns out that inflammasome activation and its consequences including cytokine storms, apoptosis and pyroptosis could be associated with the reduced sperm count as well as the structural and functional sperm defects recorded in several situations associated with male infertility suggesting that anti-inflammatory therapeutic strategies could be possibly considered to restore male fertility in future research.

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Section: Methodsmentioning

confidence: 99%

The NLRP3 inflammasome: molecular activation and regulation in spermatogenesis and male infertility; a systematic review

Tavalaee

Rahmani

Drevet

et al. 2022

Basic Clin. Androl.

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“…Among these remaining studies, 166 studies were excluded from our meta‐analysis because 82 studies were unrelated to varicocele, 23 studies did not report sufficient data for synthesis, 33 studies were conducted in animals, 17 studies were reviews associated with varicocele, 6 studies were letters, and 5 studies were guidelines for varicocele. Finally, a total of 12 studies were included in our meta‐analysis with a total of 845 patients diagnosed with varicocele and 2,377 healthy controls, all meeting our inclusion criteria (Blumer et al., 2008; Blumer et al., 2012; Camargo et al., 2021; Dieamant et al., 2017; Finelli et al., 2021; Jellad et al., 2021; La Vignera et al., 2012; Santana et al., 2019; Smith et al., 2006; Talebi et al., 2008; Tanaka et al, 2020; Wu et al., 2009).…”

Section: Resultsmentioning

confidence: 99%

Effect of varicocele on sperm DNA damage: A systematic review and meta‐analysis

Zhang

et al. 2021

Andrologia

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The updated meta‐analysis was conducted to further verify the effect of varicocele on sperm DNA damage, supplying clinicians and researchers with high‐grade evidence. The sperm DNA damage was evaluated by DNA fragmentation index (DFI), associated with the male fertility capability tightly. PubMed, Web of Science and Cochrane Library were searched extensively for eligible studies with the search terms: varicocele, sperm DNA and sperm DNA damage. Finally, a total of 12 studies were included in our meta‐analysis with a total of 845 patients diagnosed with varicocele and 2,377 healthy controls. A statistical difference of DFI between varicocele patients and healthy controls was found after pooling the data ((Standardised mean difference) SMD: 1.40, 95%CI: 0.83–1.98, p < .0001), using the random effect model. We conducted subgroup analysis according to study region (Brazil and Other countries), detection methods of DFI (TUNEL, Comet, and SCSA), sample size (<50 and >50) and age (<30 and >30 years), based on substantial heterogeneity among eligible studies. The stability of pooled results was verified by sensitivity analysis. All these statistical analyses were conducted using Stata version 16.0. In conclusion, patients diagnosed with clinical varicocele had higher DFI than healthy controls, which means varicocele could impair sperm DNA, consequently the fertility potential of affected men.

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“…IL‐1α and IL‐1β have been reported to be increased in the VC model 33,34 . Camargo et al found that IL‐1, IL‐18 and caspase‐1 decreased in semen after varicocelectomy by ELISAs 35 . Micheli et al tested seminal plasma samples and found that sperm apoptosis, IL‐6, and TNF‐α were increased in VC patients 36 .…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction

et al. 2022

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Objectives:To evaluate the expression, potential functions and mechanisms of long noncoding RNAs (lncRNAs) in the pathogenesis of varicocele (VC)-induced spermatogenic dysfunction. Materials and Methods:We established a rat model with left experimental VC and divided rats into the sham group, the VC group, and the surgical treatment group (each group, n = 10). Haematoxylin and eosin (HE) staining and sperm quality were analysed to evaluate spermatogenesis function. LncRNA expression profiles were analysed using lncRNA-Seq (each group n = 3) and validated using quantitative realtime polymerase chain reaction (each group n = 10). Correlation analysis and gene target miRNA prediction were used to construct competing endogenous RNA network.The regulated signalling pathway and spermatogenic dysfunction of differentially expressed lncRNAs (DE lncRNAs) were validated by Western blot.Results: HE detection and sperm quality analysis showed that VC could induce spermatogenic dysfunction. Eight lncRNAs were upregulated and three lncRNAs were downregulated in the VC group compared with the sham group and surgical treatment group. The lncRNA of NONRATG002949.

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Seminal inflammasome activity in the adult varicocele

Cited by 13 publications

References 55 publications

The NLRP3 inflammasome: molecular activation and regulation in spermatogenesis and male infertility; a systematic review

The NLRP3 inflammasome: molecular activation and regulation in spermatogenesis and male infertility; a systematic review

Effect of varicocele on sperm DNA damage: A systematic review and meta‐analysis

Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction

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