Seminal fluid transferrin as an index of gonadal function in men

Barthélémy, C.; Khalfoun, B.; Guillaumin, J. M.; Lecomte, P.; Bardos, P.

doi:10.1530/jrf.0.0820113

Cited by 28 publications

(14 citation statements)

References 18 publications

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“…Although Kawakami et al [17] reported that the mean concentration of transferrin in normal canine testis was 321 ± 32 ng/g, testicular transferrin in animals other than dogs has not been measured. A positive correlation exists between seminal plasma transferrin concentrations and sperm density and between total seminal transferrin and total numbers of sperm in ejaculate in humans [2,6] and cattle [9]. But in the present study no significant correlation was found between seminal transferrin and the sperm count in dogs, indicating that canine seminal transferrin is not an index of gonadal function unlike human and bovine seminal transferrins.…”

Section: Discussioncontrasting

confidence: 87%

“…The seminal transferrin concentrations in normal dogs seem to be much lower than those in normal humans [2,6,12] or cattle [9]. Although Kawakami et al [17] reported that the mean concentration of transferrin in normal canine testis was 321 ± 32 ng/g, testicular transferrin in animals other than dogs has not been measured.…”

Section: Discussionmentioning

confidence: 99%

“…Transferrin is secreted by the Sertoli cells [13,22] and plays an important role in spermatogenesis by providing iron to developing germ cells, which have specific transferrin receptors [11,24]. Seminal plasma transferrin is known to be a good index of gonadal function in humans [2,6] and cattle [9], although 20% and 40% of transferrin in semen is contributed by the accessory sex glands of humans [12] and cattle [9], respectively.…”

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confidence: 99%

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Seminal Plasma Lactoferrin but Not Transferrin Reflects Gonadal Function in Dogs

Kikuchi

Mizoroki

Kubo

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. Lactoferrin purified from canine seminal plasma by a three-step chromatography procedure had a molecular mass of 75.2 kDa and cross-reacted with antiserum to equine seminal plasma lactoferrin. Seminal plasma lactoferrin concentrations were determined by a competitive enzyme-linked immunosorbent assay (ELISA) by using rabbit anti-equine lactoferrin antibody and alkaline phosphataselabeled goat anti-rabbit IgG antibody in 14 normal dogs and found to range from 12 to 197 µg/ml, with a mean value of 77 ± 59 µg/ml (the mean ± SD). Seminal plasma transferrin concentrations were determined by a sandwich ELISA with goat antibody to canine serum transferrin and alkaline phosphatase-conjugated goat anti-canine transferrin antibody and found to range from 0.32 to 12.6 µg/ml, with a mean value of 2.44 ± 3.25 µg/ml. The lactoferrin concentration significantly correlated with the sperm concentration (r=0.7025, P<0.01), but there was no significant correlation between the seminal plasma transferrin concentration and sperm density. These results indicate that seminal plasma lactoferrin, but not transferrin, reflects gonadal function. KEY WORDS: canine, lactoferrin, seminal plasma, sperm, transferrin.J. Vet. Med. Sci. 65(6): 679-684, 2003 Lactoferrin is an iron-binding protein (molecular mass of 80 kDa) that is found in various exocrine fluids, blood plasma and specific granules of neutrophils [15]. Lactoferrin has been identified in the seminal plasma of humans [10,19], swine [21] and horses [14]. Lactoferrin seems to be synthesized mainly in the epididymis of mice [27], swine [16] and horses [7], and it is present in the prostate and seminal vesicles, but not in the epididymis, of humans [26]. The physiological role of lactoferrin in the male reproductive tract remains unclear, although lactoferrin has been identified as the sperm-coating antigen in humans [10] and swine [16,21].Transferrin is homologous to lactoferrin and is a major iron transport protein in the circulation [1], and is also found to be present in the seminal plasma of humans [12] and cattle [9]. Transferrin is secreted by the Sertoli cells [13,22] and plays an important role in spermatogenesis by providing iron to developing germ cells, which have specific transferrin receptors [11,24]. Seminal plasma transferrin is known to be a good index of gonadal function in humans [2, 6] and cattle [9], although 20% and 40% of transferrin in semen is contributed by the accessory sex glands of humans [12] and cattle [9], respectively.No information is available about either lactoferrin or transferrin in canine semen. In the present study, an enzyme immunoassay for measuring the concentrations of lactoferrin and transferrin in canine seminal plasma was developed and the relationships between seminal plasma lactoferrin and transferrin concentrations, the number of sperm and sperm motility were examined. MATERIALS AND METHODSSeminal samples: Ejaculate was obtained from 14 normal mature dogs (age unknown). Seminal properties including sperm number and sperm moti...

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Section: Discussioncontrasting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Seminal Plasma Lactoferrin but Not Transferrin Reflects Gonadal Function in Dogs

Kikuchi

Mizoroki

Kubo

et al. 2003

The Journal of Veterinary Medical Science

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“…A few reports have been published on the use of 31P-NMR on human or mammalian spermatozoa in vitro (Robitaille et al, 1987;Bahl et al, 1988;Bretan et al, 1989). (Barthélémy et al, 1988 …”

Section: Discussionmentioning

confidence: 99%

1H nuclear magnetic resonance studies of seminal plasma from fertile and infertile men

Hamamah

François²,

Barthélémy³

et al. 1993

Reproduction

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“…Until recently, no clinical parameter was available for the evaluation of the function of Sertoli cells. Recent work, however, suggests that trans ferrin (TRF) levels in the seminal plasma may serve as a functional parameter of these cells [4][5][6][7][8][9], TRF concentrations of the seminal plasma obtained from normozoospermic, oligozoospermic and azoosper mic patients were measured and evaluated as a probable potential marker of Sertoli cell function.Received: January 14, 1992 Accepted: March 17. 1992…”

mentioning

confidence: 99%

Seminal Transferrin Levels in Seminal Plasma of Fertile and Infertile Men

Çek¹,

Cûrgûl²,

Ertaş³

et al. 1992

Urol Int

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Seminal plasma transferrin levels as well as serum FSH and LH levels were measured in fertile (normozoospermic) and infertile (oligozoospermic and azoospermic) men in an effort to find out whether transferrin levels correlated with sperm density and serum FSH and LH levels. Seminal plasma transferrin levels were found to be significantly lower (p < 0.0005) in the infertile group than the fertile group. Seminal plasma transferrin levels can be regarded as a functional parameter of Sertoli cell function in men.

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Seminal fluid transferrin as an index of gonadal function in men

Cited by 28 publications

References 18 publications

Seminal Plasma Lactoferrin but Not Transferrin Reflects Gonadal Function in Dogs

Seminal Plasma Lactoferrin but Not Transferrin Reflects Gonadal Function in Dogs

1H nuclear magnetic resonance studies of seminal plasma from fertile and infertile men

Seminal Transferrin Levels in Seminal Plasma of Fertile and Infertile Men

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