Seminal Clusterin Gene Expression Associated with Seminal Variables in Fertile and Infertile Men

Zalata, Adel; Elsamanoudy, Ayman Z; Shaalan, Dalia; El-Baiomy, Youssef; Taymour, Mai; Mostafa, Taymour

doi:10.1016/j.juro.2012.06.012

Cited by 35 publications

(29 citation statements)

References 28 publications

(19 reference statements)

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“…Even when compared with clusterin expression in the reproductive fluids of other species, we have studied using the same 2D SDS-PAGE approach, such as the bovine (Moura et al 2007(Moura et al , 2010, ovine , and domestic swine (S. scrofa; V R Cadavid, However, the precise functional meaning of such event is unclear. Clusterin is multifunctional, acting as a chaperone (Humphreys et al 1999), protecting reproductive tract cells against apoptosis (Bailey et al 2002) or complement attack (Meri & Jarva 2001), and assisting with removal of damaged sperm in the epididymis of men (Zalata et al 2012). The chaperone activity of clusterin is consistent with its propensity to interact with different proteins in vivo (Carver et al 2003) and in acoordance with the network analysis we presently show.…”

Section: Proteins Associated With Protection Of Sperm Integrity and Fsupporting

confidence: 79%

Protein profile of the seminal plasma of collared peccaries (Pecari tajacu Linnaeus, 1758)

Santos¹,

Sousa²,

Martins³

et al. 2014

Reproduction

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This study was conducted to characterize the major proteins of the peccary seminal plasma, based on the semen samples collected from nine adult and reproductively sound animals. Our approach included the use of two-dimensional electrophoresis followed by Coomassie blue staining and analysis of polypeptide maps with PDQuest Software (Bio-Rad). Proteins were identified by tandem mass spectrometry (LC-MS/MS). We detected 179 protein spots per gel and 98 spots were identified by mass spectrometry, corresponding to 23 different proteins. The combined intensity of those spots accounted for 56.2G6% of the intensities of all spots and 60.9% of the intensities of spots presented in every protein map. Protein spots identified as clusterin represented 19.7G8.3% of the integrated optical densities of all spots detected in the seminal plasma maps. There was a negative association (rZK0.87; P!0.05) between the intensity of a clusterin spot and the percentage of sperm with functional membrane. Spermadhesin porcine seminal plasma protein 1 and bodhesin 2 comprised 5.4G1.9 and 8.8G3.9% of the total intensity of all spots respectively. Many proteins appeared in a polymorphic pattern, such as clusterin (27 spots), epididymal secretory glutathione peroxidase (ten spots), inter-a-trypsin inhibitor (12 spots), and IgG-binding protein (ten spots), among others. In conclusion, we presently describe the major seminal plasma proteome of the peccary, which exhibits a distinct high expression of clusterin isoforms. Knowledge of wild species reproductive biology is crucial for an understanding of their survival strategies and adaptation in a changing environment.

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Section: Proteins Associated With Protection Of Sperm Integrity and Fsupporting

confidence: 79%

Protein profile of the seminal plasma of collared peccaries (Pecari tajacu Linnaeus, 1758)

Santos¹,

Sousa²,

Martins³

et al. 2014

Reproduction

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“…In the current study, it was found that sCLU negatively correlates with sperm morphology, protamine deficiency, and DNA fragmentation since less sCLU was detected in the seminal plasma of infertile patients. Although extensive research has been previously done on the relationship between CMA 3 -positivity, DNA damage, seminal CLU gene expression, and infertility (Zini et al, 2001;Nasr-Esfahani et al, 2004Muciaccia et al, 2012;Zalata et al, 2012), to our knowledge this is the first report on the correlation between sCLU of seminal plasma, sperm parameters, protamine deficiency, and DNA fragmentation in humans.…”

Section: Discussionmentioning

confidence: 93%

Correlation between human clusterin in seminal plasma with sperm protamine deficiency and DNA fragmentation

Salehi

Akbari

Heidari

et al. 2013

Molecular Reproduction Devel

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Seminal proteins can be considered as factors that control fertilization. Clusterin is one such protein that has been implicated in many activities, including apoptosis inhibition, cell cycle control, DNA repair, and sperm maturation. In this study, the relationship between human secretory clusterin (sCLU) in seminal plasma with sperm parameters, protamine deficiency, and DNA fragmentation was investigated. Semen samples were collected from 63 Iranian men, and semen analysis was performed according to World Health Organization criteria and computer aided semen analysis (CASA). The concentration of sCLU in seminal plasma was measured by enzyme-linked immunosorbant assay (ELISA), protamine deficiency was determined by chromomycin A3 staining (CMA3 ), and sperm DNA fragmentation was checked by sperm chromatin dispersion (SCD) assay. The level of sCLU in seminal fluid of fertile patients was 48.3 ± 38.6 ng/ml and in infertile patients was 15.5 ± 9.7 ng/ml; this difference was significant (P < 0.001). sCLU correlated negatively with protamine deficiency, sperm DNA fragmentation, and abnormal morphology. In conclusion, seminal clusterin can be considered as a marker for the quick assessment of semen quality in male infertility studies.

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“…Clusterin (CLU), a heterodimeric, disulfide-linked glycoprotein, has been suggested as an alternate marker for seminal oxidative stress (40). It is interesting that, as with the other HSPs described previously, CLU was found at higher protein levels in sperm that resulted in poor blastocyst development.…”

Section: Figurementioning

confidence: 87%

“…1). Others have reported that CLU exerts a small heat-shock-protein-like stress-induced chaperone activity and is an extremely sensitive cellular biosensor to environmental and/or oxidative stress (40,41). The conventional CLU is expressed in a wide variety of tissues and cells and has been linked to several biologic processes, including aging, tumorigenesis, neurodegeneration, and cardiovascular and metabolic syndromes (41).…”

Section: Figurementioning

confidence: 99%

“…Clusterin is synthesized and secreted by the testis, epididymis, and seminal vesicles, and it is generally agreed that CLU is mainly present on the surface of abnormal spermatozoa (42)(43)(44)(45). Increased CLU expression also has been associated with increased sperm DNA fragmentation (40). Additionally, CLU has been reported in normal spermatozoa, but the different localization and origin of this CLU suggest it to be a novel form compared with the conventional CLU (46).…”

Section: Figurementioning

confidence: 99%

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Toward the identification of a subset of unexplained infertility: a sperm proteomic approach

McReynolds

Dzieciątkowska

Stevens

et al. 2014

Fertility and Sterility

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Seminal Clusterin Gene Expression Associated with Seminal Variables in Fertile and Infertile Men

Abstract: CLU gene expression was significantly increased in the semen samples of infertile men. It correlated negatively with sperm count, motility, acrosin activity, linearity index and linear velocity, and positively with the percent of sperm abnormal forms and DNA fragmentation.

Cited by 35 publications

References 28 publications

Protein profile of the seminal plasma of collared peccaries (Pecari tajacu Linnaeus, 1758)

Protein profile of the seminal plasma of collared peccaries (Pecari tajacu Linnaeus, 1758)

Correlation between human clusterin in seminal plasma with sperm protamine deficiency and DNA fragmentation

Toward the identification of a subset of unexplained infertility: a sperm proteomic approach

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