Semiconductor sensor embedded microfluidic chip for protein biomarker detection using a bead-based immunoassay combined with deoxyribonucleic acid strand labeling

Lin, Yi‐Ying; Peng, Po-Yu

doi:10.1016/j.aca.2015.03.002

Cited by 15 publications

(8 citation statements)

References 28 publications

(29 reference statements)

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“…[ 56 ] Among them, electrochemical immunosensors are the most broadly used for quantitative analysis of clinical biomarkers. [ 57 – 60 ] Their main innovation resides in the introduction of nanomaterials in their design (i.e., gold nanoparticle, carbon nanomaterial, quantum dots, etc. ), that significantly increase the solid surface area for affinity reagents contributing to high sensitivity performance.…”

Section: Potentials and Challenges Of Affinity-based Proteomicsmentioning

confidence: 99%

“…[ 61 ] Therefore, immunosensors may soon provide convincing advantages over standard immunoassays, including miniaturized and renewal devices, sensitivity, rapid detection, degree of multiplexing, cost [ 62 ] and automation. [ 57 ] Important parameters for clinical laboratories will, however, remain in the performance characteristics such as precision, robustness, reproducibility and sustainability of these devices. Consequently, more work needs to be dedicated to meet clinical requirements before novel immunosensors can become a platform within clinical diagnostics, in particular for the bedside as point of care systems.…”

Section: Potentials and Challenges Of Affinity-based Proteomicsmentioning

confidence: 99%

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Immunocapture strategies in translational proteomics

Fredolini

Byström

et al. 2015

Expert Review of Proteomics

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Aiming at clinical studies of human diseases, antibody-assisted assays have been applied to biomarker discovery and toward a streamlined translation from patient profiling to assays supporting personalized treatments. In recent years, integrated strategies to couple and combine antibodies with mass spectrometry-based proteomic efforts have emerged, allowing for novel possibilities in basic and clinical research. Described in this review are some of the field’s current and emerging immunocapture approaches from an affinity proteomics perspective. Discussed are some of their advantages, pitfalls and opportunities for the next phase in clinical and translational proteomics.

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Section: Potentials and Challenges Of Affinity-based Proteomicsmentioning

confidence: 99%

Section: Potentials and Challenges Of Affinity-based Proteomicsmentioning

confidence: 99%

Immunocapture strategies in translational proteomics

Fredolini

Byström

et al. 2015

Expert Review of Proteomics

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“…Another study reported a magnetic bead-based immunoassay for urinary protein biomarker detection in a microfluidic device [30]. Magnetic beads with epoxy groups were conjugated with antibodies to capture apolipoprotein A1, a bladder cancer biomarker.…”

Section: On-chip Sample Preparation Methodsmentioning

confidence: 99%

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Sonker

Sahore

Woolley

2017

Analytica Chimica Acta

138

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Microfluidics is a vibrant and expanding field that has the potential for solving many analytical challenges. Microfluidics show promise to provide rapid, inexpensive, efficient, and portable diagnostic solutions that can be used in resource-limited settings. Researchers have recently reported various microfluidic platforms for biomarker analysis applications. Sample preparation processes like purification, preconcentration and labeling have been characterized on-chip. Additionally, improvements in microfluidic separation techniques have been reported for cellular and molecular biomarkers. This review critically evaluates microfluidic sample preparation platforms and separation methods for biomarker analysis reported in the last two years. Key advances in device operation and ability to process different sample matrices in a variety of device materials are highlighted. Finally, current needs and potential future directions for microfluidic device development to realize its full diagnostic potential are discussed.

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“…In addition, Lin et al developed a semiconductor sensor with magnetic beads to conduct protein detection on the urinary protein apolipoprotein A1. The 120-nm magnetic beads were used to create an immunoassay for protein isolation [ 45 ]. First, to isolate the proteins from the sample, a magnetic bead-based immunoassay was performed with the antibody-conjugated beads.…”

Section: Improving the Binding Capacity Of The Capture Antibodiesmentioning

confidence: 99%

“…This way the charged complex at the bottom of the magnetic beads was forced to make contact with the sensor surface. By using longer DNA fragments as secondary labels, the generated signal was 15 times greater than that without the labels, and the platform was able to reach a LOD of 12.5 ng/mL [ 45 ]. This method allowed for a low LOD and a portable device size, however requiring equipment for the fabrication of the semiconductor sensor.…”

Section: Improving the Binding Capacity Of The Capture Antibodiesmentioning

confidence: 99%

Pushing the detection limits: strategies towards highly sensitive optical-based protein detection

Momenbeitollahi

Cloet

2021

Anal Bioanal Chem

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Proteins are one of the main constituents of living cells. Studying the quantities of proteins under physiological and pathological conditions can give valuable insights into health status, since proteins are the functional molecules of life. To be able to detect and quantify low-abundance proteins in biofluids for applications such as early disease diagnostics, sensitive analytical techniques are desired. An example of this application is using proteins as biomarkers for detecting cancer or neurological diseases, which can provide early, lifesaving diagnoses. However, conventional methods for protein detection such as ELISA, mass spectrometry, and western blotting cannot offer enough sensitivity for certain applications. Recent advances in optical-based micro- and nano-biosensors have demonstrated promising results to detect proteins at low quantities down to the single-molecule level, shining lights on their capacities for ultrasensitive disease diagnosis and rare protein detection. However, to date, there is a lack of review articles synthesizing and comparing various optical micro- and nano-sensing methods of enhancing the limits of detections of the antibody-based protein assays. The purpose of this article is to critically review different strategies of improving assay sensitivity using miniaturized biosensors, such as assay miniaturization, improving antibody binding capacity, sample purification, and signal amplification. The pros and cons of different methods are compared, and the future perspectives of this research field are discussed.

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Semiconductor sensor embedded microfluidic chip for protein biomarker detection using a bead-based immunoassay combined with deoxyribonucleic acid strand labeling

Cited by 15 publications

References 28 publications

Immunocapture strategies in translational proteomics

Immunocapture strategies in translational proteomics

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Pushing the detection limits: strategies towards highly sensitive optical-based protein detection

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