Semiconductor quantum dots for in vitro diagnostics and cellular imaging

Jin, Zongwen; Hildebrandt, Niko

doi:10.1016/j.tibtech.2012.04.005

Cited by 145 publications

(106 citation statements)

References 65 publications

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“…For fluorescent biosensors, the optical properties of QDs are an excellent basis for signaling (5). However, the heavy-metal content of standard-composition QDs (typically CdSe and ZnS) means that toxicity might prohibit wide-scale use, particularly in clinical applications where disposal costs of toxic heavy metals could be prohibitive.…”

Section: Nanoparticle Coresmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

893

686

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Colloidal nanoparticle biosensors have received intense scientific attention and offer promising applications in both research and medicine. We review the state of the art in nanoparticle development, surface chemistry, and biosensing mechanisms, discussing how a range of technologies are contributing toward commercial and clinical translation. Recent examples of success include the ultrasensitive detection of cancer biomarkers in human serum and in vivo sensing of methyl mercury. We identify five key materials challenges, including the development of robust mass-scale nanoparticle synthesis methods, and five broader challenges, including the use of simulations and bioinformatics-driven experimental approaches for predictive modeling of biosensor performance. The resultant generation of nanoparticle biosensors will form the basis of high-performance analytical assays, effective multiplexed intracellular sensors, and sophisticated in vivo probes.Evolution has given rise to organisms of staggering complexity. Our now extensive knowledge of biological systems pales in comparison to the remaining mysteries. Unraveling these requires tools that probe the molecular machinery of life and provide detailed feedback on complex networks of subtle interactions. Such tools are cornerstones of biomedical research and practice, and improvements in these lead directly to a better understanding of fundamental biology, monitoring of health, and diagnosis of disease. Colloidal nanoparticle biosensors are a class of biological probe that will not only yield improved biological sensing but also provide a step change in our ability to probe the biomolecular realm. Nanoparticles can act as high-performance sensors because nanomaterials exhibit unique and useful behaviors not present in their bulk form: for example, bright tunable fluorescence from semiconductor nanoparticles and localized surface plasmon resonance (LSPR) phenomena in metallic nanoparticles. These particles exhibit intense responses to incident light (or other stimuli), and the ability to modulate this response by interaction with target analytes makes them excellent biosensor signal transducers. Outputs can be quantitative or qualitative depending on the functionality of

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Section: Nanoparticle Coresmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

893

686

View full text Add to dashboard Cite

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“…174,268,270,430 QDs can be linked to antibodies, oligonucleotides, aptamers, and streptavidin for specific binding to tissue biomarkers 430 and are particularly useful to study tumor heterogeneity. 174,214,215 Advantages of QDs in comparison to standard organic fluorochromes include narrow emission spectra (less interference in multiplex analysis), higher photostability (at least 10 hours), and higher fluorescent signal per unit of light absorbed. 98,268,430 In addition, QD IHC is more accurate and precise at low protein concentration than traditional IHC.…”

mentioning

confidence: 99%

When Tissue Antigens and Antibodies Get Along

2013

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Once focused mainly on the characterization of neoplasms, immunohistochemistry (IHC) today is used in the investigation of a broad range of disease processes with applications in diagnosis, prognostication, therapeutic decisions to tailor treatment to an individual patient, and investigations into the pathogenesis of disease. This review addresses the technical aspects of immunohistochemistry (and, to a lesser extent, immunocytochemistry) with attention to the antigen-antibody reaction, optimal fixation techniques, tissue processing considerations, antigen retrieval methods, detection systems, selection and use of an autostainer, standardization and validation of IHC tests, preparation of proper tissue and reagent controls, tissue microarrays and other high-throughput systems, quality assurance/quality control measures, interpretation of the IHC reaction, and reporting of results. It is now more important than ever, with these sophisticated applications, to standardize the entire IHC process from tissue collection through interpretation and reporting to minimize variability among laboratories and to facilitate quantification and interlaboratory comparison of IHC results.

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“…Metal nanoparticles (NPs) (51) and semiconductor quantum dots (QDs) (52,53) and related materials, such as single-wall carbon nanotubes (SWNTs) (54,55), are increasingly used for preclinical in vitro and in vivo (imaging) diagnostics (56)(57)(58). Therapeutic applications have been slower to develop (59).…”

Section: Inorganic Nanoparticles and Related Nanomaterials In Biomedimentioning

confidence: 99%

Nanotechnologies for biomedical science and translational medicine

Heath

2015

Proc. Natl. Acad. Sci. U.S.A.

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In 2000 the United States launched the National Nanotechnology Initiative and, along with it, a well-defined set of goals for nanomedicine. This Perspective looks back at the progress made toward those goals, within the context of the changing landscape in biomedicine that has occurred over the past 15 years, and considers advances that are likely to occur during the next decade. In particular, nanotechnologies for health-related genomics and single-cell biology, inorganic and organic nanoparticles for biomedicine, and wearable nanotechnologies for wellness monitoring are briefly covered.nanotechnology | biotechnology | nanomedicine

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Semiconductor quantum dots for in vitro diagnostics and cellular imaging

Cited by 145 publications

References 65 publications

Colloidal nanoparticles as advanced biological sensors

Colloidal nanoparticles as advanced biological sensors

When Tissue Antigens and Antibodies Get Along

Nanotechnologies for biomedical science and translational medicine

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