Semibatch and continuous fructooligosaccharides production by <i>Aspergillus</i> sp. N74 in a mechanically agitated airlift reactor

Caicedo, Luis A.; Silva, Edelberto; Sánchez, Oscar F.

doi:10.1002/jctb.2095

Cited by 14 publications

(12 citation statements)

References 34 publications

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“…Ritsema et al (2006) proposed that this motif could influence the entry of the acceptor sucrose when fructose is retained in the enzyme. In that sense, fungal FTases have a "chimeric" domain that supports the findings of invertase activity in fungal FTases under certain conditions (Yun, 1996;Sangeetha et al, 2005;Sanchez et al, 2008aSanchez et al, , 2008bCaicedo et al, 2009). The analysis of this motif in fungi FTases supports our hypothesis that they were generated from an ancestral dicot invertase since they have the "chimeric" (F-and S-type) domain.…”

Section: Single Amino Acids Responsible For Specific Characteristicssupporting

confidence: 78%

“…During the last years, we have worked in the production of FOS by using an A. oryzae strain (Sanchez et al, 2008a(Sanchez et al, , 2008bCaicedo et al, 2009), and recently the ftase gene from that strain was cloned and the tertiary structure of the enzyme was modeled (Rodriguez et al, 2010). To continue the understanding and optimization of FOS production, in this study we conducted a computational analysis of reported sequences for FTases from a diverse source of organisms, such as plants, fungi and bacteria.…”

Section: Introductionmentioning

confidence: 98%

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Computational analysis of the fructosyltransferase enzymes in plants, fungi and bacteria

Alméciga-Díaz

Gutiérrez

Bahamon

et al. 2011

Gene

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Section: Single Amino Acids Responsible For Specific Characteristicssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 98%

Computational analysis of the fructosyltransferase enzymes in plants, fungi and bacteria

Alméciga-Díaz

Gutiérrez

Bahamon

et al. 2011

Gene

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“…FTase produces FOS from sucrose by transferring one to three molecules of fructose. Several microorganisms have FTase, such as Fusarium sp., Aspergillus sp., Aureobasidium sp., Penicillium sp., Arthrobacter sp., Zymomonas mobilis , Bacillus macerans , Candida, Kluyveromyces , and Saccharomyces cerevisia e [41,44,45,46,47,48]. Among these microorganisms, Aspergillus niger and Aureobasidium pullulans are mostly used in the industry [49].…”

Section: Production Of Prebioticsmentioning

confidence: 99%

Prebiotics: Definition, Types, Sources, Mechanisms, and Clinical Applications

Davani-Davari

Negahdaripour

Karimzadeh

et al. 2019

Foods

938

674

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Prebiotics are a group of nutrients that are degraded by gut microbiota. Their relationship with human overall health has been an area of increasing interest in recent years. They can feed the intestinal microbiota, and their degradation products are short-chain fatty acids that are released into blood circulation, consequently, affecting not only the gastrointestinal tracts but also other distant organs. Fructo-oligosaccharides and galacto-oligosaccharides are the two important groups of prebiotics with beneficial effects on human health. Since low quantities of fructo-oligosaccharides and galacto-oligosaccharides naturally exist in foods, scientists are attempting to produce prebiotics on an industrial scale. Considering the health benefits of prebiotics and their safety, as well as their production and storage advantages compared to probiotics, they seem to be fascinating candidates for promoting human health condition as a replacement or in association with probiotics. This review discusses different aspects of prebiotics, including their crucial role in human well-being.

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“…After conducting the enzymatic reaction, the dry weight biomass was determined for both, small and bioreactor scales. This biomass was washed with 50 mM phosphate buffer (pH 5.5) and dried for 48 h at 105 ℃ (Caicedo et al., 2009).…”

Section: Methodsmentioning

confidence: 99%

“…These authors determined the optimal pH (5.5), temperature (60 ℃) and sucrose concentration (>55% w / v ) for FOS production using A. oryzae N74 whole cells. In addition, FOS production at continuous, semibatch and batch operation in an agitated airlift reactor was evaluated, obtaining FOS production yields that ranged from 47.8% to 66.4% depending on the biomass concentration and operational mode (Caicedo et al., 2009; Sánchez et al., 2008). Recently, it was cloned the A. oryzae N74 ftase gene as a previous step for the production of the recombinant enzyme in Pichia pastoris (GenBank accession numbers GU145136 and GU145137) (Rodríguez et al., 2011), and the computational analysis of the encoded FTase allowed to classify it in the glycoside hydrolase family GH32 (Alméciga-Díaz et al., 2011).…”

Section: Introductionmentioning

confidence: 99%

Use of spent osmotic solutions for the production of fructooligosaccharides by Aspergillus oryzae N74

Ruiz

Klotz²,

Serrato

et al. 2013

Food sci. technol. int.

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In the food industry, osmotic dehydration can be an important stage to obtain partially dry foodstuffs. However, the remaining spent osmotic solution at the end of the process could become a waste with an important environmental impact due to the large amount of organic compounds that it might contain. Since one of the most important osmotic agents used in osmotic dehydration is sucrose, this spent osmotic solution could be used to be biotransformed to produce fructooligosaccharides by a fructosyltransferase. This study evaluated the production of fructooligosaccharides using the fructosyltransferase produced by Aspergillus oryzae N74, and the spent osmotic solution that resulted in the osmotic dehydration of Andes berry (Rubus glaucus) and tamarillo (Cyphomandra betacea). Assays were conducted at small and bioreactor scales, using spent osmotic solution with or without re-concentration. At small scale no significant difference (p > 0.05) was observed in the fructooligosaccharides production yield, ranging from 31.18% to 34.98% for spent osmotic solution from tamarillo osmotic dehydration, and from 33.16% to 37.52% for spent osmotic solution from Andes berry osmotic dehydration, using either the SOS with or without re-concentration. At bioreactor scale the highest fructooligosaccharides yield of 58.51 ± 1.73% was obtained with spent osmotic solution that resulted from tamarillo osmotic dehydration. With the spent osmotic solution from Andes berry osmotic dehydration the yield was 49.17 ± 2.82%. These results showed the feasibility of producing fructooligosaccharides from spent osmotic solution that is considered a waste in food industry.

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Semibatch and continuous fructooligosaccharides production by Aspergillus sp. N74 in a mechanically agitated airlift reactor

Cited by 14 publications

References 34 publications

Computational analysis of the fructosyltransferase enzymes in plants, fungi and bacteria

Computational analysis of the fructosyltransferase enzymes in plants, fungi and bacteria

Prebiotics: Definition, Types, Sources, Mechanisms, and Clinical Applications

Use of spent osmotic solutions for the production of fructooligosaccharides by Aspergillus oryzae N74

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