Semi-refined carrageenan promotes generation of reactive oxygen species in leukocytes of rats upon oral exposure but not in vitro

Tkachenko, Anton; Kot, Yurii; Kapustnyk, V. A.; Myasoedov, Valeriy; Makieieva, Nataliia; Chumachenko, Tetyana; Onishchenko, Anatolii; Lukyanova, Yevgeniya; Наконечна, Оксана Анатоліївна

doi:10.1007/s10354-020-00786-7

Cited by 20 publications

(11 citation statements)

References 31 publications

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“…There is evidence that neutrophils and reactive oxygen species (ROS) generated by them play an important role in the development of experimental carrageenaninduced inflammation, in particular, peritonitis [36]. Moreover, we have demonstrated that food-grade carrageenan may enter neutrophils in vitro [37]. Therefore, we believe that intense apoptosis of granulocytes found in this study can develop due to excessive ROS generation induced by carrageenans since ROS accumulation is known to trigger apoptosis in neutrophils [38].…”

Section: Discussionmentioning

confidence: 54%

Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation

Tkachenko¹,

Pogozhykh²,

Onishchenko³

et al. 2021

J. Pharm. Nutr. Sci.

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Gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) have been shown to scavenge reactive oxygen species (ROS), making them a promising therapeutic agent in inflammation.This study aims to assess the effects of VNPs administered orally on E407a-induced inflammation.Materials and Methods: Fragments of the small intestine of 8 rats treated orally with a carrageenan-containing food additive E407a at a dose of 140 mg / kg of weight during 2 weeks, 8 animals orally exposed to both E407a and VNPs at a dose of 20 µg / kg of weight during the same period of time, and 8 control rats were stained routinely and immunostained for CD3 and CD68 with the subsequent immunohistochemical scoring. Moreover, analysis of viability and cell death modes of granulocytes was performed by flow cytometry using Annexin V and 7aminoactinomycin D (7-AAD).Results: Oral exposure to the food additive E407a resulted in the development of enteritis associated with altered small intestinal morphology, infiltration of the lamina propria with macrophages and T-lymphocytes, and activation of peripheral blood granulocyte apoptosis. VNPs administered against the background of E407a-induced slight intestinal inflammation improved small intestinal morphology, decreased infiltration rate of the immune cells mentioned above without affecting the intensity of granulocyte apoptosis. Conclusion:Oral administration of VNPs ameliorates E407a-induced enteritis.

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Section: Discussionmentioning

confidence: 54%

Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation

Tkachenko¹,

Pogozhykh²,

Onishchenko³

et al. 2021

J. Pharm. Nutr. Sci.

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“…It is important to mention that immune cells are more sensitive to carrageenan cytotoxic effects. Our earlier studies demonstrate the uptake of semi-refined carrageenan by leukocytes and anti-apoptotic bcl-2 upregulation in lymphocytes in vitro, as well as excessive ROS production and activated apoptosis of circulating leukocytes after E407a ingestion in vivo [25,26]. Moreover, semi-refined carrageenan increases the metabolic activity of splenocytes and bone marrow cells at lower concentrations compared with fibroblasts (unpublished data).…”

Section: Discussionmentioning

confidence: 85%

“…It has been reported that carrageenan toxicity is mediated by the TLR4-and NF-kB-associated pathways, NLRP3 inflammasome pathway, ROS-mediated damage and, hence, upregulation of pro-inflammatory cytokines [25,[29][30][31]. The role of pathways outlined above in immune cells is more pronounced compared with fibroblasts, which can be speculated to explain the difference in cytotoxic effects of semi-refined carrageenan on different types of cells.…”

Section: Discussionmentioning

confidence: 97%

Effects of E407a on the viability, metabolic and functional activity of dermal fibroblasts

et al. 2021

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“…However, there are some advances in elucidating such mechanisms. Both cell culture-based and animal-based studies have provided evidence that reactive oxygen species (ROS) are involved in carrageenan-mediated cytotoxicity [7][8][9][10]. Moreover, carrageenan-induced ROS production is believed to affect other pro-inflammatory intracellular signaling pathways, e.g.…”

Section: Introductionmentioning

confidence: 99%

The study of phospholipid bilayer of cell membranes in leukocytes incubated with high concentrations of the food additive E407a

et al. 2021

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The aim of the study was to assess the impact of semi-refined carrageenan (E407a) on the hydrophobic region of phospholipid bilayer in cell membranes of leukocytes exposed to the solution with high concentrations of this food additive.Material and methods: Fluorescent probe (2-phenylphenanthro[9,10-d]-1,3-oxazole) was used to investigate the influence of E407a on the state of lipid bilayer in leukocytes extracted from rats and treated with a 5% solution of the food additive E407a during 4 hours.Results: The shapes of the probe fluorescence spectra did not differ in leukocytes of rats treated with the 5% solution of semi-refined carrageenan and white blood cells of control samples. Such findings suggest that exposure to the E407a solution causes no changes in the proton-donor ability of the media in the lipid membranes of leukocytes in the region where the probe locates.Conclusion: Exposure of white blood cell suspensions to the semirefined carrageenan solution does not affect the membrane hydration of the hydrophobic region of leukocyte phospholipid bilayer.

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Semi-refined carrageenan promotes generation of reactive oxygen species in leukocytes of rats upon oral exposure but not in vitro

Cited by 20 publications

References 31 publications

Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation

Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation

Effects of E407a on the viability, metabolic and functional activity of dermal fibroblasts

The study of phospholipid bilayer of cell membranes in leukocytes incubated with high concentrations of the food additive E407a

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