1999
DOI: 10.4269/ajtmh.1999.60.183
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Semi-nested, multiplex polymerase chain reaction for detection of human malaria parasites and evidence of Plasmodium vivax infection in Equatorial Guinea.

Abstract: Abstract. A semi-nested, multiplex polymerase chain reaction (PCR) based on the amplification of the sequences of the 18S small subunit ribosomal RNA (ssrRNA) gene was tested in a field trial in Equatorial Guinea (a hyperendemic focus of malaria in west central Africa). The method uses a primary PCR amplification reaction with a universal reverse primer and two forward primers specific for the genus Plasmodium and to mammals (the mammalian-specific primer was included as a positive control to distinguish uninf… Show more

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Cited by 155 publications
(144 citation statements)
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“…In two African studies, PCR prevalence of P. malariae was 23.3% and 39.2%, and P. ovale prevalence was 6.9% and 9.3%. By contrast, LM detected no non-P. falciparum infections in the first study [58] and 18.7% P. malariae and 2.8% P. ovale in the second [59]. Findings consistent with these observations have been generated recently in a study of >1200 Kenyans.…”
Section: Improved Diagnosis Of P Malariae and P Ovale Infections Bysupporting
confidence: 84%
“…In two African studies, PCR prevalence of P. malariae was 23.3% and 39.2%, and P. ovale prevalence was 6.9% and 9.3%. By contrast, LM detected no non-P. falciparum infections in the first study [58] and 18.7% P. malariae and 2.8% P. ovale in the second [59]. Findings consistent with these observations have been generated recently in a study of >1200 Kenyans.…”
Section: Improved Diagnosis Of P Malariae and P Ovale Infections Bysupporting
confidence: 84%
“…These data differ from results of others who have reported the presence of P. vivax in Equatorial Guinea and Angola. In these studies, the P. vivaxinfected individuals were either Duffy positive (37) or diagnosed solely based on P. vivax-specific PCR products without sequence verification (38). Given the frequency of off-target amplification even with P. vivax-specific primers (SI Appendix, Table S6), any P. vivax diagnosis in central Africa should be confirmed by sequence analysis.…”
Section: Discussionmentioning
confidence: 99%
“…[13][14][15] The PCR was also found to be more sensitive than the QBC™ 15 method and dipstick assays (sensitivity ‫ס‬ 100% versus 88%). 16 Compared with examination of thin blood smears, the specificity of the PCR was excellent, 12,[17][18][19][20] and greater than that of the QBC™ or dipstick assays. 15 While the PCR appears to have overcome the two major problems of the diagnosis of imported malaria, namely sensitivity and specificity, the routine feasibility of this method still remained to be assessed.…”
Section: Introductionmentioning
confidence: 99%