Semi-conservative and non-conservative replication of DNA in temperature-sensitive mouse L-cells

Sheinin, Rose; Guttman, S.

doi:10.1016/0005-2787(77)90130-7

Cited by 21 publications

(16 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ts2 cell is therefore DNA'1/ Gi'1 [3,5,28]. These three ts gene products play no obligatory role in DNA repair repli cation [11,29], On the basis of these considerations and the data presented here it could be concluded that replication of mouse adenovirus DNA can occur in the absence of normal, semicon servative cellular DNA synthesis and does not require the participation of functional cellular DNA topoisomerase II orthe /.sCI or Is 2 gene products. This is not surprising in view of recent evidence which shows that human adenovirus DNA carries genetic in formation for specific proteins that initiate, prime and replicate the viral genome [30,31].…”

mentioning

confidence: 70%

“…The conditions for the maintenance, growth, and temperature manipulation of the ts mouse L cells and their wild-type (WT-4) parent have been described [10][11][12]. The same is true for the BALB/c 3T3 mouse fibroblast [13] and its ts 2 derivative [14,15].…”

mentioning

confidence: 99%

“…Both gene products are required for semiconservative DNA replication, which occurs normally during the DNA-synthetic, or S, phase of the cell duplication cycle [3,5,11,12,24], Because they arrest at unique stages early in S phase, they are designated DNA'7S'\ The is 2 mouse fibroblast arrests at the Gi/S interface [28] upon temperature inactivation of a polypeptide which acts to effect S-phase entry, perhaps by facilitating interaction of DNA polymerase-a and DNA primase [27]. The ts2 cell is therefore DNA'1/ Gi'1 [3,5,28].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Mouse Polyoma Virus and Adenovirus Replication in Mouse Cells Temperature-Sensitive in DNA Synthesis

1985

Self Cite

View full text Add to dashboard Cite

Mouse adenovirus multiplies, apparently without impediment, in temperature-inactivated ts A1S9, ts C1 and ts 2 mouse fibroblasts. Thus, the DNA of mouse adenovirus can replicate in the absence of functional DNA topoisomerase II, a DNA-chain-elongation factor, and a protein required for traverse of the Gi/S interface, respectively, encoded in the ts A1S9, ts C1 and ts2 genetic loci. These results are compared with those obtained with polyoma virus.

show abstract

mentioning

confidence: 70%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Mouse Polyoma Virus and Adenovirus Replication in Mouse Cells Temperature-Sensitive in DNA Synthesis

1985

Self Cite

View full text Add to dashboard Cite

show abstract

“…WT-4 cells (== 2 x 10 cells) grown at 34 or 38.5"C and generally labelled in their DNA with [3~]dThd m d [I4c]dThd, respectively, (3,4) were combined and processed as just described for measurement of the sedimentation behaviow of the now doubly labelled DNA. Data analogous to those described in Results were obtained and are therefore discussed there.…”

Section: Velmi~y Sedimentation Of Nuclear D Mmentioning

confidence: 99%

“…Incubation of the dn8/Sb* (1) ts A1S9 mutant mouse L cell (2) at the npt results in inhibition of DNA replication first detectable 6-8 h pts (3,4). Inactivation of the ts A1S9 gene product allows DNA synthesis to continue on already initiated R.U.…”

Section: Introductionmentioning

confidence: 98%

Novobiocin sensitivity and chromatin conformation in wild-type and temperature-sensitive mouse L cells

Colwill¹,

Sheinin²

1982

Can. J. Biochem.

View full text Add to dashboard Cite

Colwill, R. W. & Sheinin, R. (1982) Novobiocin sensitivity and chromatin conformation in wild-type and temperature-sensitive mouse L cells. Can. J. Biochem. 60, 195-263 The ts A1S9 cell is a dmts mutant of mouse L cells which exhibits enhanced sensitivity to killing by the antibiotic novobiocin, as compared with the parental wild-type (WT-4) cell, another dna*' mouse L cell (ts Cl), and other mammalian cells. Such a response was not detected with other cytotoxic agents tested. Novobiocin inhibits DNA synthesis in ts AlS9 cells, at concentrations which have little or no effect on RNA or protein synthesis. Enhanced novobiocin sensitivity is not due to altered cellular permeability to the h g , since ts A1 S9 cells do not take up more antibiotic than do WT-4 or ts' aR mouse L cells. A ts' revertant of ts A1S9 cells exhibits a wild-type pattern of novobiocin sensitivity, suggesting that novobiocin resistance may be encoded in the ts AlS9 locus. These findings led to a study of DNA conformation which revealed that the nuclear, chromatin-bound DNA of temperature-inactivated ts A1 S9 cells sediments differently in ethidium bromide sucrose density gradients than does that of WT-4 cells grown at 38.5"C. These observations suggest that expression of the ts A 1 S9 defect results in a more highly constrained conformation of the DNA, relative to that in control cells, and that in this configuration the DNA may be unable to act as template for replication.Colwill, R. W. & Sheinin, R. (1982) Novobiscin sensitivity and chromatin conformation in wild-type and temperature-sensitive mouse L cells. Can. J. Biochem. 60, 195-203 La cellule ts A 1 S9 est mn dnats mutant des cellules L de soavis qui montre une sensibiliti accrue aux effets cytotoxiques d'un mtibiotique, la novobiocine, quand on la compare % celle de la cellule parent.de de type sauvage (WT-4)' celle d'une autre cellule L d d s de souris (ts C1) et h celle d'autres cellules mmaliennes. B'amtres agents cytotoxiques essayts ne donnent pas une telle rkponse. La novobiocine inhibe la synthkse de le DNA dans les cellules ts AlS9 des concentrations qui exercent peu ou pas d'effet sur la synthkse du RNA ou des proteines. La sensibilitt accrue h la novobiocine n'est pas causte par une alttration de la pm6abilitt cellulaire % l'tgwd de la drogue, car les cellules ts A1S9 n'incorporent pas plus d'antibiotique que ne le font les cellules WT-4 ou les cellules L ts* AR de souris. Un rtvertmt ts' des cellules ts AlS9 montre mne sensibilitt $ la novobiocine semblable B celle des cellules du type sauvage, ce qui sugg&re que la resistance la novobiocine puisse $tre codte dans le locus ts AlS9. Ces domtes ont conduit une ttude de la conformation du DNA; elle nous a rtvC1C que le DNA nucltaire, lie a la chromatine, des cellules ts A1S9 inactivtes par la temp$rature stdimente de fason diffkrente dans le bromure d'tthidium contenant des gradients de densitt de saccharose que ne le fait le DNA des cellules WT-4 croissant % 38,5"C. D'aprks ces observations, l'expression du dkfaut ts A1S9 rtsu...

show abstract

Molecular cloning, primary structure and expression of the human X linked A1S9 gene cDNA which complements the ts A1S9 mouse L cell defect in DNA replication.

Zacksenhaus

Sheinin

1990

The EMBO Journal

View full text Add to dashboard Cite

The temperature‐sensitive ts A1S9 mutation of mouse L cells was previously shown to affect nuclear DNA replication and to be complemented by active and inactive human X chromosomes in human‐ts A1S9 somatic cell hybrids. We report the isolation of cDNA clones which correct the ts A1S9 lesion, using as a probe a genomic fragment derived from the human A1S9 locus. The nucleotide sequence of the A1S9 cDNA encompasses a single open reading frame of 2409 bp which could encode a heretofore unreported protein of 90 393 daltons. Southern blot hybridization of the A1S9 cDNA probe with DNA from various species revealed homologous sequences in vertebrates but not in yeast. Northern blot analysis of serum‐starved, synchronized cells demonstrated that the A1S9 gene was expressed at a relatively low level in quiescent cells and at a higher and constant level throughout the cell cycle. Human cell lines harbouring increasing numbers of inactive X chromosomes (47, XXX, 49, XXXXX) were found to express the A1S9 gene at the same level as control cells (45, X), suggesting that the gene does not escape X chromosome inactivation.

show abstract

Semi-conservative and non-conservative replication of DNA in temperature-sensitive mouse L-cells

Cited by 21 publications

References 31 publications

Mouse Polyoma Virus and Adenovirus Replication in Mouse Cells Temperature-Sensitive in DNA Synthesis

Mouse Polyoma Virus and Adenovirus Replication in Mouse Cells Temperature-Sensitive in DNA Synthesis

Novobiocin sensitivity and chromatin conformation in wild-type and temperature-sensitive mouse L cells

Molecular cloning, primary structure and expression of the human X linked A1S9 gene cDNA which complements the ts A1S9 mouse L cell defect in DNA replication.

Contact Info

Product

Resources

About