Semi-autonomous real-time programmable fluorescence lifetime segmentation with a digital micromirror device

Aluko, Justin; Perrin, Camille; Devauges, Viviane; Nedbal, Jakub; Poland, Simon P.; Matthews, Daniel R.; Whittaker, Janek; Ameer-Beg, Simon

doi:10.1364/oe.26.031055

Cited by 1 publication

(1 citation statement)

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“…Current developments in laser systems that can be found in literature address scarcely this key-point and keep the excitation wavelength fixed by the technology involved, thus presenting a restrained interest in MPM. In that context, a need in wideband excitation systems for MPM appeared, combined with specific detection solutions such as standard spectral detection or spatial autonomous real-time programmable fluorescence segmentation [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Advanced biomedical multiphoton fluorescence microscopy with a large band excitation system

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Lefort

2020

Biomedical Spectroscopy, Microscopy, and Imaging

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Commercial multiphoton microscopes currently include standard and bulk titanium-sapphire laser sources (Ti: Sa) delivering a narrow spectral bandwidth of 10 nm at the full-width at half maximum. Such a spectral width precludes the instantaneous imaging of more than four fluorophores simultaneously in optimal conditions of target. Femtosecond ultrawideband laser system (UWLS) appeared since fifteen years with an approach of spectral broadening of Ti: Sa pulses into a photonic crystal fiber associated with a dispersion compensation system. In the current work, we demonstrate the interest of an alternative laser solution, compact, cheap, simple and turn-key based on a UWLS delivering a unique spectral excitation bandwidth named supercontinuum. We have coupled this system to a passive filtering setup. Thanks to this unique spectral bandwidth, eleven fluorophores are imaged with multiphoton processes without any modification on the excitation parameters. .

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Section: Introductionmentioning

confidence: 99%