Semi-automated shear stress measurements in developing embryonic hearts

Elahi, Sahar; Blackburn, Brecken J.; Lapierre-Landry, Maryse; Gu, Shi; Rollins, Andrew M.; Jenkins, Michael W.

doi:10.1364/boe.395952

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…The studies that address the role of blood flow in the biomechanical regulation of cardiogenesis include computational models created using structural information to estimate the stress along heart tissue at stages of development [8,9]. Other studies of blood flow in cardiac development measure flow velocity at a particular location, such as the outflow tract, and show discrepancies in flow patterns associated with cardiac defects [10][11][12][13]. Technological capabilities to study the biomechanics at play during early mammalian cardiogenesis have begun to develop with methods for Optical Coherence Tomography (OCT), a noninvasive, high speed imaging modality with the spatial resolution and volumetric imaging depth necessary to study live cardiodynamics in mouse models of human development and disease [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Quantitative OCT angiography for dynamic volumetric analysis in embryonic cardiovascular system

McCown,

Larina

2024

Dynamics and Fluctuations in Biomedical Photonics XXI

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Congenital heart defects represent one in three congenital defects and are present in an estimated 1.8% of newborns worldwide. Mouse models provide an irreplaceable resource for studying mammalian development and early cardiogenesis. Early dynamics of circulation during heart development are understood to influence heart formation, but quantitative assessment of spatially and temporally resolved blood flow in the highly dynamic embryonic hearts remains challenging. Optical coherence tomography (OCT) uniquely provides the high speed, spatial resolution, and imaging depth necessary to study biomechanics early in heart development. Building off advancements in Doppler OCT and quantitative OCT angiography, we present dynamic, volumetric (4D) speed analysis of blood flow in the embryonic cardiovascular system. Our new flow tracking method is based on time-at-pixel measurements, blood cell size statistics, and the periodicity of the cardiac cycle. We characterize the effects of detection thresholds to account for variation in signal intensity, such as due to lower light penetration over tissue depth throughout the heart or when comparing between embryos. We incorporate segmentation methods using speckle variance between cycles to expand the analysis from manually defined blood vessels to dynamic regions of blood flow. With these advancements, we quantify blood flow speed within the embryonic mouse heart as it beats. The presented method will allow biomechanical studies of early blood flow in regulating mammalian heart development.

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Section: Introductionmentioning

confidence: 99%

Quantitative OCT angiography for dynamic volumetric analysis in embryonic cardiovascular system

McCown,

Larina

2024

Dynamics and Fluctuations in Biomedical Photonics XXI

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“…With the development of medical imaging techniques, detailed geometric models can be generated for human fetal hearts [ 35 ], chicken and zebrafish embryos [ 36 , 37 , 38 , 39 ]. Although there are some challenges in imaging the highly dynamic heart tissues, it is possible to generate four dimensional (4D) models, including three dimensions in space and one dimension in time domain [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Computational Modeling of Blood Flow Hemodynamics for Biomechanical Investigation of Cardiac Development and Disease

Salman

Yalcin

2021

JCDD

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The heart is the first functional organ in a developing embryo. Cardiac development continues throughout developmental stages while the heart goes through a serious of drastic morphological changes. Previous animal experiments as well as clinical observations showed that disturbed hemodynamics interfere with the development of the heart and leads to the formation of a variety of defects in heart valves, heart chambers, and blood vessels, suggesting that hemodynamics is a governing factor for cardiogenesis, and disturbed hemodynamics is an important source of congenital heart defects. Therefore, there is an interest to image and quantify the flowing blood through a developing heart. Flow measurement in embryonic fetal heart can be performed using advanced techniques such as magnetic resonance imaging (MRI) or echocardiography. Computational fluid dynamics (CFD) modeling is another approach especially useful when the other imaging modalities are not available and in-depth flow assessment is needed. The approach is based on numerically solving relevant physical equations to approximate the flow hemodynamics and tissue behavior. This approach is becoming widely adapted to simulate cardiac flows during the embryonic development. While there are few studies for human fetal cardiac flows, many groups used zebrafish and chicken embryos as useful models for elucidating normal and diseased cardiogenesis. In this paper, we explain the major steps to generate CFD models for simulating cardiac hemodynamics in vivo and summarize the latest findings on chicken and zebrafish embryos as well as human fetal hearts.

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Semi-automated shear stress measurements in developing embryonic hearts

Cited by 2 publications

References 20 publications

Quantitative OCT angiography for dynamic volumetric analysis in embryonic cardiovascular system

Quantitative OCT angiography for dynamic volumetric analysis in embryonic cardiovascular system

Computational Modeling of Blood Flow Hemodynamics for Biomechanical Investigation of Cardiac Development and Disease

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