We investigate the primordial curvature perturbation by the observation of the dark matter substructure. Assuming a bump in the spectrum of the curvature perturbation in the wave number of k > 1 Mpc −1 , we track the evolution of the host halo and subhalos in a semianalytic way. Taking into account possible uncertainties in the evaluation of the tidal stripping effect on the subhalo growth, we find a new robust bound on the curvature perturbation with a bump from the number of observed dwarf spheroidal galaxies in our Galaxy and the observations of the stellar stream. The upper limit on the amplitude of the bump is Oð10 −7 Þ for k ∼ 10 3 Mpc −1 . Furthermore, we find the boost factor, which is crucial for the indirect detection of dark matter signals, is up to Oð10 4 Þ due to the bump that is allowed in the current observational bounds.