Semen quantitative and qualitative parameters depending on age in AI bucks

Moru, Diana; Neagu, Iuliana; Tăpăloagă, Dana; Călin, Ion; Tapaloaga, Paul Rodian

doi:10.1016/j.jbiotec.2016.05.251

Cited by 1 publication

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“…Nevertheless, these adverse seasonality effects on semen production may be reversed or manipulated by using subcutaneous melatonin implants, especially during a non-breeding season by artificially mimicking day length effect [ 16 ]. Furthermore, semen quality can be influenced by donor age, indicating that sexual maturation and semen quality increase as a male gets older due to the increase in testicular size [ 17 ]. However, some reports have shown no difference in semen quality between yearlings and mature bucks [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

The Re-Addition of Seminal Plasma after Thawing Does Not Improve Buck Sperm Quality Parameters

Ohaneje

Osuagwuh

Álvarez‐Rodríguez

et al. 2021

Animals

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In order to achieve a higher post-thaw buck sperm quality, an approach in the thawing protocol of cryopreserved sperm doses under in vitro capacitation conditions mimicking the in vivo female environment was studied. Therefore, functional and kinetic characteristics of buck thawed sperm from males of different ages, the season of collection, and melatonin implanted males in the non-breeding season were assessed after 3 h of incubation in an in vitro fertilization (IVF) media with 20% of buck seminal plasma (SP). Previously, fresh ejaculates were collected via artificial vagina from eight males of the Cabra Blanca de Rasquera breed during two consecutive years in breeding and non-breeding periods. Prior to semen collection in non-breeding seasons, males were split into two groups: one group was implanted with melatonin, while the other was not. In each group, semen samples were pooled, centrifuged, and diluted in an extender containing 15% powdered egg yolk and 5% glycerol before freezing. After thawing, sperm were washed and incubated in three different media: (a) control media (modified phosphate-buffered saline (PBS), (b) IVF commercial media, and (c) IVF media + 20% SP. Sperm motility was evaluated by CASA, while plasma and acrosome membrane integrity, mitochondria activity, and DNA fragmentation were analysed by flow cytometer at 0 h and after 3 h incubation. A significant reduction in motility, mitochondrial activity, plasma, and acrosome membrane integrity were observed after incubation in the presence of SP, although similar to that observed in IVF media alone. DNA integrity was not affected under in vitro capacitation conditions, regardless of SP addition. In conclusion, the addition of SP failed to improve post-thaw buck sperm quality under in vitro conditions irrespective of male age, the season of collection, and melatonin implant.

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Section: Introductionmentioning

confidence: 99%