Semen quality and reproductive performance after artificial insemination with boar sperm stored in a gelatin-supplemented extender

Corcini, Carine Dahl; Moreira, Fabiana; Pigozzo, Rudy; Varela, Antônio Sérgio; Torres, N.U.; Lucia, Thomaz

doi:10.1016/j.livsci.2010.11.019

Cited by 13 publications

(16 citation statements)

References 14 publications

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“…Coy et al (2009) used a plant extract to increase the viscosity of a medium for swine spermatozoa and observed an increase in the STR index and the percentage of progressively motile spermatozoa, although they also reported lower VCL, VSL and VAP values in comparison to the control medium. Corcini et al (2011) found an increase in the percentage of motile spermatozoa after adding gelatin to the BTS extender for short term (three days) preserved swine semen. In our study, this parameter was not different between the extenders.…”

Section: Discussionmentioning

confidence: 88%

“…In our study, this parameter was not different between the extenders. The literature suggests that changes to motility parameters are dependent on the agent used to increase the viscosity (because it determines whether liquefaction will occur at the temperature at which motility is measured), as well as the degree of viscosity (Corcini et al, 2011;Coy et al, 2009;Salvador et al, 2006;Yániz et al, 2005). Different viscous media, therefore, influence the motility characteristics of the spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

“…However, research in boars about this subject is scarce. Corcini et al (2011) observed that adding gelatin to the short-term Beltsville Thawing Solution (BTS) extender for boar semen produced a less marked fall in the percentage of motile and morphologically normal spermatozoa during the preservation period compared to the samples to which no gelatin was added, although no significant differences between extenders were observed in either parameter (Corcini et al, 2011). Coy, Gadea, Rath and Hunter (2009) showed that the re-suspension of boar spermatozoa in a viscous medium increased the stability of the sperm membrane, decreased reactive oxygen species production and increased the capacity of spermatozoa to penetrate oocytes in vitro.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Increasing Extender Viscosity Improves the Quality of Cooled Boar Semen

Gil¹,

Barón²,

Guerrero³

et al. 2014

JAS

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The use of several types of gelling extenders for the storage of semen from several domestic species in the solid state has been shown to have beneficial effects on some semen quality parameters. The objective of this study was to evaluate the effect of a new high-viscosity semen extender, Zoosperm ND-5 3D ® (Import-Vet, Centelles, Spain), on the the quality of boar spermatozoa at preserved at 17ºC for 7 days. Sodium alginate was used for the first time to increase the viscosity of the extender for the liquid storage of boar semen. The same extender, but without increased viscosity, was used as a control extender (Zoosperm ND-5 ® , Import-Vet, Centelles, Spain). Sixteen ejaculates from four Pietrain boars were evaluated for motility (by the CASA system), and for viability, acrosome status, plasma membrane fluidity, externalization of phosphatidylserine at the plasma membrane of the spermatozoa and mitochondrial membrane potential (by flow cytometry). In samples diluted with the Zoosperm ND-5 3D ® viscous extender, the STR (straightness) parameter and the number of progressively motile spermatozoa were higher compared to those of the non-viscous extender (p < 0.05). In addition, the number of spermatozoa with damaged acrosomes, an unstable sperm plasma membrane and externalization of phosphatidylserine at the plasma membrane was lower in samples treated with the viscous extender (p < 0.05). In conclusion, an increase in extender viscosity improves quality of boar spermatozoa following long-term storage.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Increasing Extender Viscosity Improves the Quality of Cooled Boar Semen

Gil¹,

Barón²,

Guerrero³

et al. 2014

JAS

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“…The pre‐oestrous LH surge triggers, in turn, the synthesis and the secretion of cellulose that degrades the cellulose‐based wall. A recent study by Corcini et al. (2011) proposes a hybrid semen treatment process (dilution + immobilization) adding commercial gelatin to semen extender: the gelatin‐entrapped spermatozoa maintain high quality in terms of morphology and motility, and the gelatin‐treated spermatozoa had significantly greater percentages of normal spermatozoa until 96 h of storage.…”

Section: Future Perspectivesmentioning

confidence: 99%

New Aspects of Boar Sperm Encapsulation

Faustini

2011

Reprod Domestic Animals

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Contents The study takes into account the main steps and techniques for boar semen encapsulation, to optimize the instrumental insemination interventions. The use of cheap, biocompatible polymers as alginate can assure a regular, constant release of spermatozoa in the sow reproductive system, avoiding the double/triple intervention of insemination and reducing the employ of disposable materials. The encapsulation/microencapsulation of semen can therefore be the starting point of new, innovative systems of pig reproduction management.

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“…With respect to natural mating, AI offers a number of advantages: cost reduction, hygiene improvement, preventive determination of semen quality and better fertility (Okere and Nelson 2005). Nevertheless, AI efficiency is limited by difficulties related to swine reproductive physiology: the sow is characterized by a seasonal variation in the length of oestrus, the interval from oestrus to ovulation and the duration of ovulation (Belstra et al 2004), while boar sperm cells are highly susceptible to dilution and cooling procedures so that AI is routinely performed with liquid-stored semen at 15-20°C for 1-5 days (Johnson et al 2000;Corcini et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Sperm Encapsulation from 1985 to Date: Technology Evolution and New Challenges in Swine Reproduction

Perteghella

Vigani

Crivelli

et al. 2015

Reprod Domestic Animals

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Contents In the last 30 years, encapsulation technology has been applied to different species to minimize the loss of spermatozoa after artificial insemination. In particular, the vehiculation of boar sperm cells in barium alginate membrane has proved a valid strategy to reduce the risk of polyspermy and optimize in vivo fertilizing yields. Controlled release of male gametes into the female genital tract has reduced the minimum fertilizing dose of spermatozoa. Notwithstanding these results, encapsulation has not yet reached commercial application, largely due to the additional costs of production. However, encapsulation could be useful in advanced reproductive technology, such as sex sorting, to store sorted boar semen. The controlled release of flow cytometrically sorted spermatozoa could be a promising strategy to reduce the number of cells necessary for each insemination and hence allow the widescale use of sex sorting in this species.

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Semen quality and reproductive performance after artificial insemination with boar sperm stored in a gelatin-supplemented extender

Cited by 13 publications

References 14 publications

Increasing Extender Viscosity Improves the Quality of Cooled Boar Semen

Increasing Extender Viscosity Improves the Quality of Cooled Boar Semen

New Aspects of Boar Sperm Encapsulation

Sperm Encapsulation from 1985 to Date: Technology Evolution and New Challenges in Swine Reproduction

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