Semen processing and artificial insemination in health management of small ruminants

Cseh, Sándor; Faigl, V.; Amiridis, G.S.

doi:10.1016/j.anireprosci.2012.01.014

Cited by 68 publications

(46 citation statements)

References 38 publications

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“…Cervical outflow should be avoided [15] [46], especially when it is plentiful [46]. In the present work, most ewes inseminated after the first fold did not present any cervical outflow (Table 6) and resulted in a high fertility rate (81.5%).…”

Section: Cervical Outflowmentioning

confidence: 48%

“…Reproduction control and AI hold several advantages to commercial flocks: they improve farm management [1] [2] [3] and production [4]- [9], reproduction data recording accuracy [4] [7], genetic progress and animals production value [4] [5] [6] [7] [10]- [19] and allow the use of temporary physic, physiology or behavior defective animals as breeders [7] [14], precocious detection of infertility or subfertility [5] [20], control sexual transmitted diseases [4] [6] [9] [10] [14] [15] [21] and improve profitability [4] [6] [7] [22]. Nevertheless this technic is scarcely used in the Northeast of Portugal due to breeders' age, poor education, size and scarce property ownership, tradition, among other factors.…”

Section: Introductionmentioning

confidence: 99%

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Estrus Synchronization and Artificial Insemination with Fresh and Chilled Semen in Assaf Ewes

Fornazari¹,

Mateus²,

Correia³

et al. 2018

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This paper aims to study the efficiency of two short-term progestagen (FGA vs. MAP) + eCG treatments in estrus synchronization and artificial insemination (AI) with fresh or chilled semen in Assaf ewes fertility rate. All ewes received a subcutaneous implant of exogenous melatonin 45 days before been treated with short-term progestagens + eCG. By June 1 st , ewes were divided in two groups: half was treated with an intravaginal sponge impregnated with 20 mg of FGA and the other half with an intravaginal sponge impregnated with 60 mg of MAP. Progestagen treatments lasted for 6 days. At sponge withdraw, all ewes were injected with 750 IU of eCG. Ovarian activity was assessed by plasmatic progesterone levels before and after progestagens + eCG treatment. Semen was collected by electro ejaculation and extended with Andromed ® or OviXcell ® . AI was performed 55 hours after eCG administration with fresh or chilled semen. During AI several factors were assessed: vagina mucosa color and lubrication, external cervical Os type, cervical mucous viscosity, semen deposition place and seminal cervix outflow. Semen was deposited as deep as possible without distress or trauma cervix mucosa. All Assaf ewes presented cyclic activity before progestagen + eCG treatments (2 nd fortnight of May).Short-term progestagen + eCG treatments were equally efficient (100.0%). About 76.5% of Assaf ewes were pregnant 41 days after AI. Fertility rate was influenced by external Os type, semen deposition place and seminal cervix outflow. However, this rate was not conditioned by vaginal color or lubrication, cervical mucus viscosity, semen preservation technic and semen extender.

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Section: Cervical Outflowmentioning

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Estrus Synchronization and Artificial Insemination with Fresh and Chilled Semen in Assaf Ewes

Fornazari¹,

Mateus²,

Correia³

et al. 2018

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“…En la actualidad, existe la necesidad de mejorar y desarrollar diferentes protocolos de criopreservación espermática en caprino debido que no existen protocolos eficientes para la aplicación de éstos en la crioconservación de dosis destinadas a inseminación artificial (Cseh et al, 2012). A pesar del desarrollo de nuevas técnicas de inseminación y del relativo mejoramiento de los protocolos de congelación de semen caprino, apenas se han llegado a obtener tasas de gestación entorno al 60% con semen criopreservado (Leboeuf et al, 2000).…”

Section: Discussionunclassified

Dinámicas espermáticas subpoblacionales durante el proceso de criopreservación en eyaculados de caprino (Capra aegagrus hircus)

Barbas¹,

Horta²,

García-Herreros³

2017

SPERMOVA

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RESUMENEl objetivo del presente estudio fue determinar la dinámica espermática subpoblacional mediante la evaluación objetiva de parámetros cinéticos funcionales durante la criopreservación del semen de caprino. Se utilizaron 40 eyaculados procedentes de 8 machos en los que se evaluaron los parámetros de volumen, concentración, viabilidad, integridad acrosómica y motilidad espermática objetiva mediante sistema computarizado de análisis seminal (CASA). Se evaluaron un total de 8 parámetros cinéticos (VCL, VSL, VAP, LIN, STR, ALH, BCF y WOB) en cada etapa de manipulación de la muestra espermática durante el periodo de criopreservación [1º: semen fresco (F); 2º: 1er lavado/centrifugación (1LC); 3º: 2º lavado/centrifugación (2LC); 4º: enfriamiento o "cooling" a 4ºC (CL) y 5º: pos-descongelación a 37ºC (PD)]. Los resultados derivados de los parámetros cinéticos espermáticos se analizaron mediante análisis de componentes principales (PCA) y análisis multivariante de conglomerados para identificar la relación entre las subpoblaciones espermáticas obtenidas y los parámetros funcionales de motilidad en cada etapa. Se detectaron cuatro subpoblaciones espermáticas en la 2ª, 3ª y 5ª etapa (Sbp1LC1-Sbp1LC4, Sbp2LC1-Sbp2LC4 y SbpPD1-SbpPD4) y tres en la 1ª (SbpF1-SbpF3) y 4ª etapa (SbpCL1-SbpCL3). Según los parámetros de velocidad obtenidos en cada subpoblación diferenciada se observaron células rápidas, lentas, activas, pasivas, progresivas y no progresivas. Por otro lado, tomando en cuenta los parámetros de linealidad se observaron trayectorias espermáticas curvilíneas, rectilíneas, parabólicas y erráticas. Se observó variabilidad en las subpoblaciónes detectadas a lo largo de las etapas de manipulación evaluadas habiendo múltiples diferencias significativas entre los parámetros cinéticos obtenidos intra e inter-subpoblacionales (p<0,001) así como en la frecuencia de distribución de las subpoblaciones obtenidas entre etapas (p<0,05). En conclusión, el presente estudio confirmó la existencia de variabilidad y heterogeneidad en los parámetros cinéticos espermáticos en el eyaculado caprino a lo largo del proceso de criopreservación. La relación entre la estructura subpoblacional espermática y las diferentes etapas de manipulación fue estadísticamente significativa, especialmente en las etapas de enfriamiento o "cooling" y posdescongelación debido a que éstas alteraron de forma drástica los parámetros cinéticos espermáticos.Palabras clave: Subpoblaciones espermáticas, criopreservación, semen, caprino. ABSTRACTThe objective of the present research was to determine specific sperm subpopulational dynamics in different processing steps during cryopreservation process by using objective functional sperm kinematic descriptors in goat ejaculates. Fresh ejaculates (n=40) collected from eight bucks were analised for volume, concentration, sperm viability, acrosome integrity, and sperm motility using computer-assisted sperm analysis (CASA) system. Eight sperm kinematic descriptors (VCL, VSL, VAP, LIN, STR, BCF, ALH, and WOB) were assesse...

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“…The common practice for TAI in sheep flocks is comprised firstly by oestrous synchronization, using progestagen sponges, remained in situ for 14 days, followed by an eCG intramuscularly injection at sponges' removal. TAI is usually performed intracervically once at 54-56 h or 15-17 hours after the detected oestrus, even twice at 48-50 and 58-60 h after sponges' removal (Evans and Maxwell, 1987;Cseh et al, 2012). Pregnancy rate varies from 45 to 78%, depending on the hormonal treatment, the breed, the season, the method of AI and the number of inseminations performed (Evans and Maxwell, 1987;Chemineau and Congié, 1991;Menchaca and Rubianes, 2004;Cseh et al, 2012).…”

Section: περιληψηmentioning

confidence: 99%

“…TAI is usually performed intracervically once at 54-56 h or 15-17 hours after the detected oestrus, even twice at 48-50 and 58-60 h after sponges' removal (Evans and Maxwell, 1987;Cseh et al, 2012). Pregnancy rate varies from 45 to 78%, depending on the hormonal treatment, the breed, the season, the method of AI and the number of inseminations performed (Evans and Maxwell, 1987;Chemineau and Congié, 1991;Menchaca and Rubianes, 2004;Cseh et al, 2012). State of the art in methodology and technology currently applied in sheep artificial insemination had been reviewed by Cseh and coworkers (2012).…”

Section: περιληψηmentioning

confidence: 99%

Fertility rate of short-term progestagen pretreated ewes in relation to breed: A field study

Papadopoulos

Deligiannis

Theodosiadou

et al. 2018

J Hellenic Vet Med Soc

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The effect of short-term instead of long-term progestagen treatment on fertility of Karagouniko and Chios ewes, after natural mating or artificial insemination, was investigated. Two experiments were performed during the transition period from anοestrous to the breeding season. In the 1st experiment (natural mating, NM), Karagouniko and Chios ewes were randomly allocated into 3 groups, that were KLM (long-term progestagen treatment; n=35), KSM (short-term progestagen treatment; n=34), KSP (short-term progestagen treatment; prostaglandin; n=35) and CLM (n=40), CSM (n=35), CSP (n=38), respectively. In the 2nd experiment (intracervical artificial insemination, AI) Karagouniko and Chios ewes were randomly allocated into 3 groups, that were KLA (long-term progestagen treatment; AI at 54h; n=50), KSA1 (short-term progestagen treatment; AI at 54h; n=20), KSA2 (short-term progestagen treatment; AI at 48h; n=28) and CLA (n=40), CSA1 (n=16), CSA2 (n=20), respectively. At sponges’ removal (d0) all ewes received 400 IU eCG. Ten rams served NM, while for AI fresh diluted semen was used. Pregnancy diagnosis was performed, 45-50 days later. Ιn the 1st experiment, blood samples were collected, daily for 5 days, starting on d0, for serum progesterone assessment. Conception rate in Karagouniko ewes after NM was higher (P<0.05) in KSM (35.29%) compared to KLM (17.14%) group, but did not differ with KSP (28.57%) group, while after AI it was higher (P<0.05) in KLA (42.00%) or KSA1 (40.00%) compared to KSA2 (14.29%) group. In Chios ewes no significant differences were observed between groups either after NM [CLM (45.00%), CSM (36.84%), CSP (34.29%)] or after AI [CSA1 (50.00%), CSA2 (50.00%), CLA (45.00%)]. No significant differences were observed after NM or after AI in the litter size in both breeds. These results indicate that short-term progestagen treatment for oestrus synchronization could be applied in indigenous Greek sheep breeds, resulting in equal (Chios) or improved fertility (Karagouniko) than the common long-term one. Also, the fertility rate in ewes subjected to short-term progestagen treatment depends on the time of AI in relation to breed.

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Semen processing and artificial insemination in health management of small ruminants

Cited by 68 publications

References 38 publications

Estrus Synchronization and Artificial Insemination with Fresh and Chilled Semen in Assaf Ewes

Estrus Synchronization and Artificial Insemination with Fresh and Chilled Semen in Assaf Ewes

Dinámicas espermáticas subpoblacionales durante el proceso de criopreservación en eyaculados de caprino (Capra aegagrus hircus)

Fertility rate of short-term progestagen pretreated ewes in relation to breed: A field study

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