Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique

Ricci, Giuseppe; Perticarari, Sandra; Boscolo, Rita; Montico, Marcella; Guaschino, Secondo; Presani, Gianni

doi:10.1016/j.fertnstert.2007.11.068

Cited by 114 publications

(95 citation statements)

References 35 publications

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“…Although several studies have compared the effects of DGC and swim-up or both on sperm DNA integrity, the findings have been discordant [20,24]. Chiamchanya et al [25] compared the sperm quality Figure 2.…”

Section: Discussionmentioning

confidence: 99%

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Preparation and incubation conditions affect the DNA integrity of ejaculated human spermatozoa

Matsuura¹,

Takeuchi²,

Yoshida³

2010

Asian J Androl

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Appropriate semen processing and assessment are critical for successful infertility treatment. We investigated whether laboratory procedures including semen preparation and incubation affect sperm DNA integrity. A total of 153 infertile men were involved. Conventional semen parameters and sperm chromatin structure assay (SCSA) parameters, that is, DNA fragmentation index (%DFI) and high DNA stainability (%HDS), were assessed on the fresh ejaculated semen samples, which were treated and incubated under different conditions. Negative correlations were identified between the %DFI and sperm concentration, motility, progressive motility and morphology. A lower percentage of DFI was detected in spermatozoa when density gradient centrifugation (DGC) was followed by swimup treatment in comparison with DGC alone (P < 0.01). Although the %DFI increased in a time-dependent manner with incubation both at room temperature (RT) and at 37ºC in air, the %DFI after 24 h at RT was significantly lower than that at 37ºC (P < 0.05). Incubation with 5% CO2 was effective in maintaining sperm motility (P < 0.01); however, it induced further elevation of %DFI (P < 0.001). Thus, sperm DNA damage was associated with longer incubation periods. Interestingly, common culture conditions, such as maintaining pH and temperature, compromised the sperm DNA integrity.

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Section: Discussionmentioning

confidence: 99%

“…The tube was inclined at an angle of 45º. Following the incubation at RT for 60 min, the tube was set upright and the 50 µL of the upper interface was then gently aspirated with a Pasteur pipette [20] and examined for sperm concentration, motility and the SCSA.…”

Section: Swim-up Protocolmentioning

confidence: 99%

Preparation and incubation conditions affect the DNA integrity of ejaculated human spermatozoa

Matsuura¹,

Takeuchi²,

Yoshida³

2010

Asian J Androl

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“…Ejaculated sperm form a heterogeneous cell population with different functional characteristics. Motility is an evident feature of sperm and has been used to indicate vitality (Ricci et al, 2009), and swim-up (SU) technique permits the separation of motile sperm in an ejaculate. Indicators of well cell functioning other than motility has been observed in sperm subpopulations selected by SU, for example high viability (Ricci et al, 2009) and mitochondrial function (Grasa et al, 2004;Kotwicka et al, 2008).…”

mentioning

confidence: 99%

“…Motility is an evident feature of sperm and has been used to indicate vitality (Ricci et al, 2009), and swim-up (SU) technique permits the separation of motile sperm in an ejaculate. Indicators of well cell functioning other than motility has been observed in sperm subpopulations selected by SU, for example high viability (Ricci et al, 2009) and mitochondrial function (Grasa et al, 2004;Kotwicka et al, 2008). The integrity of sperm DNA is essential for embryonic development (Fatehi et al, 2006); however, DNA and chromatin abnormalities are present in ejaculated sperm (Evenson et al, 1980;Chohan and Hunter, 2004).…”

mentioning

confidence: 99%

Viability Tests, Active Caspase‐3 and −7, and Chromatin Structure in Ram Sperm Selected Using the Swim‐Up Procedure

Pichardo¹,

Martínez²,

Ayala-Escobar³

et al. 2010

Journal of Andrology

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Swim-up (SU) is a technique that permits the obtaining of motile sperm. Based on the sperm motility of neat ejaculates, we analyzed functional parameters, cytoplasmic esterases, and mitochondrial dehydrogenases of ram sperm using calcein acetomethylester and resazurin, respectively. Active caspase-3 and -7 and chromatin structure were evaluated in ram sperm before and after the SU process. There were no changes in any of the viability parameters after SU in neat semen samples with less or more than 25% motility. The percentage of active caspase-3 and -7 decreased after SU (68.8 6 4.6 vs 54.2 6 6.0), whereas a small but significant increase of chromatin structural abnormalities was observed (DNA fragmentation index [DFI], 287.3 6 3.1 vs 297.2 6 2.4). For the first time, the location of active caspase-3 and -7 was described for ram sperm. Notably, we found active caspases in the implantation fossa region. The presence of active caspases in neat ejaculates and the diminished presence of active caspases in SU-processed ejaculates suggest a role for caspases in motility and possibly in male fertility. The results of this study indicate that the evaluation of more than one cell-function marker is necessary to appropriately evaluate sperm quality. Furthermore, in semen samples with low motility, a lower percentage of sperm with active caspases is obtained after SU, although these sperm present increased values of DFI.

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“…The initial semen analysis was performed following the standard procedure [10]. Strict morphology was evaluated using the Kruger strict criteria.…”

Section: Semen Analysis and Fertilizationmentioning

confidence: 99%

A retrospective Comparison of Clinical Outcome Following Conventional In-Vitro Fertilization (c-IVF) vs Intracytoplasmic Sperm Injection (ICSI) for 100% Teratozoospermia Patients

Li¹,

Xue²,

Shi³

2017

Reproductive Immunol Open Acc

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Objectives: To investigate whether ICSI could improve the clinical outcomes of the 100% teratozoospermia patients. Methods:This study contained 200 IVF-ET and 216 ICSI-ET cycles from January 2013 to January 2016. The first cycle patients aged <40 years, with cycle day 3 FSH <10 mIU/mL and 8-12 antral follicles were included. Main outcome measure(s): clinical, obstetric and neonatal outcome. Results:The characteristics of the patients showed no significant differences in the female's age, Gn administration, Gn does, basal serum follicle-stimulating hormone, basal E2 value, endometrial thickness, infertile time, the number of retrieved oocytes and the number of transferred embryos between two groups (p>0.05). The proportion of blastocyst transfers was significantly higher in c-IVF group than ICSI group (41.00 versus 28.70%; p=0.008). We observed that the implantation (53.80 versus 44.85%; p=0.016) and clinical pregnancy (67.00 versus 60.19%; p=0.149) rates were higher in c-IVF group than those in ICSI group. In addition, the abortion (7.46 versus 16.15%; p=0.028) and ectopic pregnancy (0.75 versus 5.38%; p=0.028) rates were significantly lower in c-IVF group compared with ICSI group. The two groups showed no significant differences in obstetrical and neonatal outcomes (p>0.05). Conclusions:For isolated 100% teratozoospermia patients, ICSI should not be considered to improve the clinical outcomes if the semen meet c-IVF standard in the first cycle.

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Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique

Cited by 114 publications

References 35 publications

Preparation and incubation conditions affect the DNA integrity of ejaculated human spermatozoa

Preparation and incubation conditions affect the DNA integrity of ejaculated human spermatozoa

Viability Tests, Active Caspase‐3 and −7, and Chromatin Structure in Ram Sperm Selected Using the Swim‐Up Procedure

A retrospective Comparison of Clinical Outcome Following Conventional In-Vitro Fertilization (c-IVF) vs Intracytoplasmic Sperm Injection (ICSI) for 100% Teratozoospermia Patients

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