Semen Parameters and Sperm DNA Fragmentation as Causes of Recurrent Pregnancy Loss

Brahem, Sonia; Mehdi, Meriem; Landolsi, Hanène; Mougou, Soumaya; Elghezal, Hatem; Saâd, Ali

doi:10.1016/j.urology.2011.05.049

Cited by 94 publications

(82 citation statements)

References 24 publications

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“…Using sperm chromatin dispersion test (SCD), Bellver et al [3] observed that sperm DNA fragmentation was higher in the recurrent spontaneous abortion group compared with the sperm donor group. Moreover, Brahem et al [7] also indicated that sperm from men with a history of RPL had a higher incidence of DNA damage and poor motility than sperm from a control group. From large animal and human studies, Evenson and colleagues, who first described SCSA, suggested that threshold of 0-15 %.…”

Section: Discussionmentioning

confidence: 99%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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Purpose Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception. Methods Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI). Results By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL. Conclusions Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.

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Section: Discussionmentioning

confidence: 99%

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Kumar

Deka

Singh

et al. 2012

J Assist Reprod Genet

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“…Available evidence suggests that, although spermatozoa prepared using standard routine sperm preparation methods such as DDGC or swim-up may have generally lower levels of DNA fragmentation or compromised chromatin compared with spermatozoa from unprocessed semen samples, and may therefore be suitable for use in assisted reproduction techniques, such preparations may not exclude sperm that are compromised in these regards (Zini et al, 1999;Brahem et al, 2011b;Henkel, 2012;Mortimer and Mortimer, 2013). This is a particular issue for ICSI in which sperm selection relies almost exclusively on the embryologist's judgement.…”

Section: Introductionmentioning

confidence: 99%

“…On the basis of classical parameters such as concentration, viability, motility and morphology under WHO guidelines, these techniques do enrich for more fertile spermatozoa (WHO, 2010). Functional properties of DDGCenriched populations, such as DNA integrity and chromatin maturity that could explain the improved fertility of these populations of spermatozoa, are, however, usually not assessed.Available evidence suggests that, although spermatozoa prepared using standard routine sperm preparation methods such as DDGC or swim-up may have generally lower levels of DNA fragmentation or compromised chromatin compared with spermatozoa from unprocessed semen samples, and may therefore be suitable for use in assisted reproduction techniques, such preparations may not exclude sperm that are compromised in these regards (Zini et al, 1999;Brahem et al, 2011b;Henkel, 2012;Mortimer and Mortimer, 2013). This is a particular issue for ICSI in which sperm selection relies almost exclusively on the embryologist's judgement.…”

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confidence: 99%

Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid

Torabi

Binduraihem

Miller

2017

Reproductive BioMedicine Online

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ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. Accepted ManuscriptTitle: Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid 22-11-2016 Please cite this article as: Forough Torabi, Adel Binduraihem, David Miller, Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid, Reproductive BioMedicine Online (2016), http://dx.doi.org/doi: 10.1016/j.rbmo.2016.11.011. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMature spermatozoa bind hyaluronic acid in the extracellular matrix via hyaladherins.Immature spermatozoa may be unable to interact because they do not express the appropriate hyaladherins on their surface. Fresh human semen samples were fractionated using differential density gradient centrifugation (DDGC) and the ability of these fractions to bind hyaluronic acid was evaluated. The presence of sperm hyaladherins was also assessed. CD44 was located mainly on the acrosome and equatorial segment and became more restricted to the equatorial segment in capacitated spermatozoa. Hyaluronic acid-TRITC (hyaluronan acid conjugated with tetramethylrhodamine isothiocyanante), a generic hyaluronic-acid-binding reagent, labelled the membrane and the neck region, particularly after capacitation. Sperm populations obtained after DDGC or after interaction with hyaluronic acid were assessed for DNA fragmentation and chromatin maturity. Strong relationships between both measures and sperm sedimentation and hyaluronic-acid-binding profiles were revealed. Capacitation Overall success rates for assisted conception procedures, however (including ICSI), have remained relatively static, with about 24% of cycles for all age groups deliverin...

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“…Репродуктивные потери нации на сегодняшний день являются важной медико-социальной проблемой и определяют повышенный интерес многих исследо-вателей к состоянию фертильного потенциала [1][2][3][4][5]. В России процент бесплодного брака достаточно высок и колеблется от 10 до 15 % всех супружеских пар [6][7][8].…”

Section: Introductionunclassified

Modern Strategies of Infertile Male’s Treatment With Chronical Bacterial Prostatitis on the Stage of Preconception Predation in Protocols of Auxiliary Reproductive Technologies

Фаниев¹,

Shevchenko²,

Кадыров³

2017

Androl. genit. hir.

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Semen Parameters and Sperm DNA Fragmentation as Causes of Recurrent Pregnancy Loss

Cited by 94 publications

References 24 publications

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception

Sedimentation properties in density gradients correspond with levels of sperm DNA fragmentation, chromatin compaction and binding affinity to hyaluronic acid

Modern Strategies of Infertile Male’s Treatment With Chronical Bacterial Prostatitis on the Stage of Preconception Predation in Protocols of Auxiliary Reproductive Technologies

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