Semaphorin 6C expression in innervated and denervated skeletal muscle

Svensson, Anna; Libelius, Rolf; Tågerud, Sven

doi:10.1007/s10735-007-9113-6

Cited by 20 publications

(25 citation statements)

References 37 publications

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“…To eliminate the possibility that the Sfrp1-like immunoreactivity was unspecific, sections were first incubated with normal control goat IgG together with normal control rabbit IgG. Normal control IgGs from both species stained muscle endplates in a similar manner and the staining obtained was clearly co-localized and overlapping (results not shown, see Svensson et al 2008). Next, sections were incubated with the anti-Sfrp1 antibody together with normal goat IgG and the stainings observed, contrary to the case for the two Fig.…”

Section: Sfrp1 Immunoreactivitymentioning

confidence: 99%

“…Normal rabbit and goat IgGs (from non-immunized animals) can give a considerable staining of the endplate region of muscle fibers (Svensson et al 2008). The specificity of the staining observed with the Sfrp1 antibody was inferred by comparing the staining obtained with the Sfrp1 antibody and that observed with control IgGs (normal IgGs) from two different species (rabbit and goat).…”

Section: Specificity Of Sfrp1-like Immunoreactivitymentioning

confidence: 99%

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Secreted frizzled related protein 1 (Sfrp1) and Wnt signaling in innervated and denervated skeletal muscle

Svensson¹,

Norrby²,

Libelius

et al. 2008

J Mol Hist

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Wnts are secreted proteins with functions in differentiation, development and cell proliferation. Wnt signaling has also been implicated in neuromuscular junction formation and may function in synaptic plasticity in the adult as well. Secreted frizzled-related proteins (Sfrps) such as Sfrp1 can function as inhibitors of Wnt signaling. In the present study a potential role of Wnt signaling in denervation was examined by comparing the expression levels of Sfrp1 and key proteins in the canonical Wnt pathway, Dishevelled, glycogen synthase kinase 3beta and beta-catenin, in innervated and denervated rodent skeletal muscle. Sfrp1 mRNA and immunoreactivity were found to be up-regulated in mouse hemidiaphragm muscle following denervation. Immunoreactivity, detected by Western blots, and mRNA, detected by Northern blots, were both expressed in extrasynaptic as well as perisynaptic parts of the denervated muscle. Immunoreactivity on tissue sections was, however, found to be concentrated postsynaptically at neuromuscular junctions. Using beta-catenin levels as a readout for canonical Wnt signaling no evidence for decreased canonical Wnt signaling was obtained in denervated muscle. A role for Sfrp1 in denervated muscle, other than interfering with canonical Wnt signaling, is discussed.

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Section: Sfrp1 Immunoreactivitymentioning

confidence: 99%

Section: Specificity Of Sfrp1-like Immunoreactivitymentioning

confidence: 99%

Secreted frizzled related protein 1 (Sfrp1) and Wnt signaling in innervated and denervated skeletal muscle

Svensson¹,

Norrby²,

Libelius

et al. 2008

J Mol Hist

Self Cite

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“…In a recent work by Tatsumi et al (59), it was speculated that the increased expression of Sema3A observed in satellite cells in response to injury-induced muscle regeneration could serve a paracrine function regulating muscle innervation. Moreover, Sema6C was found to localize primarily at neuromuscular junctions in vivo, and the expression of Sema6C was reduced in response to denervation of skeletal muscle, indicating that other classes of semaphorins could be involved in neurogenesis of skeletal muscle (63).…”

Section: Dynamic Secretion Profile Of Tgf-␤1 -␤2 -␤3 and Associatementioning

confidence: 99%

Dynamics of the Skeletal Muscle Secretome during Myoblast Differentiation

Henningsen

Rigbolt

Blagoev

et al. 2010

Molecular & Cellular Proteomics

269

255

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During recent years, increased efforts have focused on elucidating the secretory function of skeletal muscle. Through secreted molecules, skeletal muscle affects local muscle biology in an auto/paracrine manner as well as having systemic effects on other tissues. Here we used a quantitative proteomics platform to investigate the factors secreted during the differentiation of murine C2C12 skeletal muscle cells. Using triple encoding stable isotope labeling by amino acids in cell culture, we compared the secretomes at three different time points of muscle differentiation and followed the dynamics of protein secretion. We identified and quantitatively analyzed 635 secreted proteins, including 35 growth factors, 40 cytokines, and 36 metallopeptidases. The extensive presence of these proteins that can act as potent signaling mediators to other cells and tissues strongly highlights the important role of the skeletal muscle as a prominent secretory organ. In addition to previously reported molecules, we identified many secreted proteins that have not previously been shown to be released from skeletal muscle cells nor shown to be differentially released during the process of myogenesis. We found 188 of these secreted proteins to be significantly regulated during the process of myogenesis. Comparative analyses of selected secreted proteins revealed little correlation between their mRNA and protein levels, indicating pronounced regulation by posttranscriptional mechanisms. Furthermore, analyses of the intracellular levels of members of the semaphorin family and their corresponding secretion dynamics demonstrated that the release of secreted proteins is tightly regulated by the secretory pathway, the stability of the protein, and/or the processing of secreted proteins. Finally, we provide 299 unique hydroxyproline sites mapping to 48 distinct secreted proteins and have discovered a novel hydroxyproline motif. Molecular & Cellular Proteomics 9:2482-2496, 2010.

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“…on the other hand, it has been shown that both human Sema6c and Sema6d are expressed in the skeletal muscle, and that the expression of both semaphorin genes is downregulated following denervation (40). Furthermore, it was found that hSema6c is expressed in extrasynaptic as well as in perisynaptic regions of muscles, and that the protein is concentrated at neuromuscular junctions, suggesting a role of hSema6c in neuromuscular communication (41).…”

Section: Discussionmentioning

confidence: 99%

Effects of fenofibrate on Semaphorin 6B gene expression in rat skeletal muscle

et al. 2011

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Abstract. Semaphorin 6B is a member of the semaphorin family of signaling proteins, which is implicated in a variety of biological processes, such as axon guidance and muscle regeneration. PParα is known to be a regulator of semaphorin 6B in human cancer cell lines. in this study, we examined the effect of fenofibrate as a PPARα activator on the expression of the Sema6B gene in rat skeletal muscle. a total of 18 rats were divided into two groups: one fed with standard chow (control group), and the other with fenofibrate chow (treatment group) for 4 weeks by gavage with 30 mg/ kg per day. The expression of rSema6B mrna was analyzed by qPcr. rSema6B protein content in rat skeletal muscle was measured by Western blotting. A significant down-regulation of rSema6B was observed at both the mrna and protein levels. using the chiP assay, a putative PParα binding region (PPRE) was identified in the rSema6B promoter. We conclude that the expression of Sema6B is down-regulated by fenofibarte in rat skeletal muscle.

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Semaphorin 6C expression in innervated and denervated skeletal muscle

Cited by 20 publications

References 37 publications

Secreted frizzled related protein 1 (Sfrp1) and Wnt signaling in innervated and denervated skeletal muscle

Secreted frizzled related protein 1 (Sfrp1) and Wnt signaling in innervated and denervated skeletal muscle

Dynamics of the Skeletal Muscle Secretome during Myoblast Differentiation

Effects of fenofibrate on Semaphorin 6B gene expression in rat skeletal muscle

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