SEM Observation of Wet Lily Pollen Grains Pretreated with Ionic Liquid

Komai, Fuminori; Okada, Kazuko; Inoue, Yuko; Yada, Mitsunori; Tanaka, Osamu; Kuwabata, Susumu

doi:10.2503/jjshs1.mi-008

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…In addition, electrostatic interactions seem to be involved in the adhesion of pollen grains to the surface of insect pollinators. 35 Using scanning electron microscopy (SEM), Komai et al 36 recently demonstrated that an ionic liquid can be coated uniformly on wet Lilium pollen grains, probably as a result of multiple ionic interactions. 37 Thus, we believe that the synthesized ILGs have an ideal structure for effective pollen adsorption.…”

Section: Synthesis and Characterization Of Ilgsmentioning

confidence: 99%

Materially Engineered Artificial Pollinators

Chechetka

Tange

et al. 2017

Chem

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Ionic liquid gels (ILGs), composed of an imidazolium salt and the copolymer of a polymerizable imidazolium salt and an acrylic cross-linker, were readily synthesized via both thermal and photopolymerization and exhibited unique multifunctional properties. Materially engineered artificial pollinators with ILGs are proposed for different applications, including light-triggered camouflage and flower pollination. Pollination was achieved through gel adsorption on living ants or on radiowave-controlled robots. This technology can lead to the development of an innovative artificial pollinator against the global pollination crisis.

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Section: Synthesis and Characterization Of Ilgsmentioning

confidence: 99%

Materially Engineered Artificial Pollinators

Chechetka

Tange

et al. 2017

Chem

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“…al. noted that highly concentrated RTILs can effectively hide surface features from observation and found that low concentrations of 1 % and 5 % [Ch][Lac]‐ethanolic solutions provided improved resolution in imaging hydrated, unfixed mature pollen grains of Lilium cultivar in SEM [48] …”

Section: Introductionmentioning

confidence: 99%

Electron Microscopy Imaging Applications of Room Temperature Ionic Liquids in the Biological Field: A Review

et al. 2021

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For biological imaging using electron microscopy (EM), the use of room‐temperature ionic liquids (RTILs) has been proposed as an alternative to traditional lengthy preparation methods. With their low vapor pressures and conductivity, RTILs can be applied onto hard‐to‐image soft and/or wet samples without dehydration – allowing for a more representative, hydrated state of material and opening the possibility for visualization of in situ physiological processes using conventional EM systems. However, RTILs have yet to be utilized to their full potential by microscopists and microbiologists alike. To this end, this review aims to provide a comprehensive summary of biological applications of RTILs for EM to bridge the RTIL, in situ microscopy, and biological communities. We outline future research avenues for the use of RTILs for the EM observation of biological samples, notably i) RTIL selection and optimization, ii) applications for live cell processes and iii) electron beam and ionic liquid interaction studies.

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“…In addition, ILs can also have very high electrical conductivity [3]. As such they are used in many applications which take advantage of one or both of these attributes, including wetting agents in high-vacuum electron microscopy studies [4][5][6][7], battery electrolytes [8][9][10], and as propellants for electrospray spacecraft propulsion [11][12][13][14][15][16][17][18][19]. In spacecraft that utilize IL electrospray, extreme electric fields are applied to the free surface of an IL in order to extract and accelerate ions and/or droplets.…”

Section: Introductionmentioning

confidence: 99%

Radiation-induced solidification of ionic liquid under extreme electric field

Terhune¹,

King²,

He³

et al. 2016

Nanotechnology

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An extreme electric field on the order of 10(10) V m(-1) was applied to the free surface of an ionic liquid to cause electric-field-induced evaporation of molecular ions from the liquid. The point of ion emission was observed in situ using a TEM. The resulting electrospray emission process was observed to create nanoscale high-aspect-ratio dendritic features that were aligned with the direction of the electric field. Upon removal of the stressing field the features were seen to remain, indicating that the ionic liquid residue was solidified or gelled. Similar electrospray experiments performed in a field-emission scanning electron microscope revealed that the features are created when the high-energy electron beam damages the molecular structure of the ionic liquid. While the electric field does not play a direct role in the fluid modification, the electric stress was critical in detecting the liquid property change. It is only because the electric stress mechanically elongated the fluid during the electrospray process and these obviously non-liquid structures persisted when the field was removed that the damage was evident. This evidence of ionic liquid radiation damage may have significant bearing on electrospray devices where it is possible to produce high-energy secondary electrons through surface impacts of emitted ions downstream of the emitter. Any such impacts that are in close proximity could see reflected secondary electrons impact the emitter causing gelling of the ionic liquid.

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SEM Observation of Wet Lily Pollen Grains Pretreated with Ionic Liquid

Cited by 5 publications

References 24 publications

Materially Engineered Artificial Pollinators

Materially Engineered Artificial Pollinators

Electron Microscopy Imaging Applications of Room Temperature Ionic Liquids in the Biological Field: A Review

Radiation-induced solidification of ionic liquid under extreme electric field

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