SEM Investigation of Pollen Taxa in Honeys from Autochtone Apis cerana in Godavari, Lalitpur District, Nepal

Paudayal, Khum N.; Gautam, Ishan

doi:10.3126/jnhm.v26i0.14130

Cited by 4 publications

(8 citation statements)

References 39 publications

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“…Acanthaceae has been considered as a eurypalynous family because of remarkable diversity in palynomorphs feature (Paudayal & Gautam, 2012; Perveen & Qaiser, 2010). This diversity could serve as taxonomic key for delimiting taxa at family, genus, or even species level.…”

Section: Resultsmentioning

confidence: 99%

“…Pollen spectra of honey are a tool for estimating the interaction between bees and vegetation (Paudayal & Gautam, 2015). Both are communally reliant on one another (Decourtye, Mader, & Desneux, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Family: ApiaceaePaudayal and Gautam (2012) andMenemen and Stephen (2001) reported striato-rugulate sculpturing in majority of Apiaceae taxa. Distinctive characteristics include inner and outer outlines, slit-like ectocolpi, and broad band-like costae, and most important reorganization character is bone shape pollen.…”

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confidence: 99%

“…is also agreed on reticulate sculpturing of Brassicaceae members.3.14 | Family: MeliaceaeMeliaceae is stenopalynous family. In honey analysis ofAdekanmbi and Ogundipe (2009),Paudayal and Gautam (2012),Freitas and Novais (2014),Nnamani and Uguru (2013) identified meliaceae in honey samples from Nepal, Southern Nigeria, Brazil, and Uganda. The identified tetracolporate and perforate pollen of meliaceae in the honey samples.…”

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confidence: 99%

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Scanning electron imaging of mellitophilous and allergenic pollen grain of arid and northern irrigated agroecological zones of Pakistan

Noor

Ahmad

2021

Microscopy Res & Technique

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Palynomorphic studies of 65 common mellitophilous and 16 allergenic flora of Arid and Northern irrigated agroecological zones of Pakistan are carried out in this study by using field emission scanning electron microscope (FESEM).Mellitophilous pollen were extracted from honey samples of selected sites. For collection of local allergenic pollen, previously identified allergy-causing plants were selected. Pollen morphological examination was carried out under FESEM.Diverse range of pollen shapes ranging from monad to polyad and sculpturing ranging from psilate to echinate, scabrate to reticulate, bireticulate, or echinolophate were observed. Brassicaceae, Myrtaceae, Asteraceae, Rosaceae, and Poaceae were observed to be dominant allergenic and mellitophilous families of the selected sites.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Scanning electron imaging of mellitophilous and allergenic pollen grain of arid and northern irrigated agroecological zones of Pakistan

Noor

Ahmad

2021

Microscopy Res & Technique

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“…This occurs in just one honey sample so could potentially be considered as an anomalous result, but Athyrium was also recorded in a commercial honey analysed by Valentini (2010)[ 39 ]. We cannot find any discussion in the literature of honey bees collecting material from pteridophytes, but the presence of pteridophyte spores has been recorded in a small number of palynological investigations from Nigeria [ 89 ], Nepal [ 90 ] and New Zealand [ 91 ]. The spores are typically considered to be a contaminant that has occurred at some stage in the processing of the honey.…”

Section: Discussionmentioning

confidence: 99%

Using DNA Metabarcoding to Identify the Floral Composition of Honey: A New Tool for Investigating Honey Bee Foraging Preferences

et al. 2015

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Identifying the floral composition of honey provides a method for investigating the plants that honey bees visit. We compared melissopalynology, where pollen grains retrieved from honey are identified morphologically, with a DNA metabarcoding approach using the rbcL DNA barcode marker and 454-pyrosequencing. We compared nine honeys supplied by beekeepers in the UK. DNA metabarcoding and melissopalynology were able to detect the most abundant floral components of honey. There was 92% correspondence for the plant taxa that had an abundance of over 20%. However, the level of similarity when all taxa were compared was lower, ranging from 22–45%, and there was little correspondence between the relative abundance of taxa found using the two techniques. DNA metabarcoding provided much greater repeatability, with a 64% taxa match compared to 28% with melissopalynology. DNA metabarcoding has the advantage over melissopalynology in that it does not require a high level of taxonomic expertise, a greater sample size can be screened and it provides greater resolution for some plant families. However, it does not provide a quantitative approach and pollen present in low levels are less likely to be detected. We investigated the plants that were frequently used by honey bees by examining the results obtained from both techniques. Plants with a broad taxonomic range were detected, covering 46 families and 25 orders, but a relatively small number of plants were consistently seen across multiple honey samples. Frequently found herbaceous species were Rubus fruticosus, Filipendula ulmaria, Taraxacum officinale, Trifolium spp., Brassica spp. and the non-native, invasive, Impatiens glandulifera. Tree pollen was frequently seen belonging to Castanea sativa, Crataegus monogyna and species of Malus, Salix and Quercus. We conclude that although honey bees are considered to be supergeneralists in their foraging choices, there are certain key species or plant groups that are particularly important in the honey bees environment. The reasons for this require further investigation in order to better understand honey bee nutritional requirements. DNA metabarcoding can be easily and widely used to investigate floral visitation in honey bees and can be adapted for use with other insects. It provides a starting point for investigating how we can better provide for the insects that we rely upon for pollination.

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On-chip detection and quantification of soap as an adulterant in milk employing electrical impedance spectroscopy

Das

Chakraborty

Karmakar

et al. 2018

2018 International Symposium on Devices, Circuits and Systems (ISDCS)

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Electrical Impedance Spectroscopy (EIS) technique is found to be an excellent candidate for bio-sensing and food quality monitoring applications due to its rapid, robust, cost-effective and point-of-care approach. The present research work investigates the implementation of EIS technique supported by several optical spectroscopic techniques such as Ultraviolet-Visible (UV-Vis) and Fourier Transform Mid Infrared (FT-MIR) to detect and quantify several toxic adulterants in foods and bio-consumables. A comprehensive understanding on the background theory related to the study has been developed to analyze the overall polarization of the system and the effect of frequency on complex permittivity of such system have been observed. In the current work, the technique is applied to adulterated saccharides, honey and turmeric samples through a prototype sensing device. All the corresponding measurements have been performed by dipping a custom-made parallel plate conductivity cell with unity cell constant inside the solution under test. EIS study exhibited a steady variation of electrical parameters such as Contents List of Figures (i-v) List of Tables (vi-vii) List of Symbols (viii-xii) List of Publications (xiii-xvi)

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SEM Investigation of Pollen Taxa in Honeys from Autochtone Apis cerana in Godavari, Lalitpur District, Nepal

Cited by 4 publications

References 39 publications

Scanning electron imaging of mellitophilous and allergenic pollen grain of arid and northern irrigated agroecological zones of Pakistan

Scanning electron imaging of mellitophilous and allergenic pollen grain of arid and northern irrigated agroecological zones of Pakistan

Using DNA Metabarcoding to Identify the Floral Composition of Honey: A New Tool for Investigating Honey Bee Foraging Preferences

On-chip detection and quantification of soap as an adulterant in milk employing electrical impedance spectroscopy

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