SEM (EDX) is an indispensable tool for the characterization of subcutaneous, preperitoneal and visceral adipose tissue of obese patients

Abstract

In this work the scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) elemental analysis were used for the characterization of adipose tissue samples taken from volunteer obese patients. The adipose tissue was taken from subcutaneous, preperitoneal and visceral layers of the adipose tissue. The obtained results provided information about the structural composition of adipose tissue layers in the human body, as well as the main microstructural features. It was demonstrated for the first time, to the best of our knowledge, that SEM and EDX are indispensable tools in order to investigate some special morphological features and the elemental composition of different layers of the human adipose tissue, identifying the surface microstructure and its qualitative composition. From the obtained results we concluded that such characterization of the adipose tissue is an essential step for the possible prediction of symptoms of different diseases.

Analytical methods used for the characterisation of specific features of biological tissues related with obesity: A review

“…SEM micrographs of the adipose tissue obtained from the subcutaneous layers of obese patients with different metabolic diseases. The bright spots are marked* [129] clearly informed that the calculated specific area of thickening and scarring of the connective tissue for the obese patients with different metabolic diseases was much larger than for obese and healthy people (Fig. 12).…”

“…The morphology by scanning electron microscopy (SEM) of the adipose samples was investigated in several studies [14,79,129,130]. The adipose tissue was taken from volunteer obese patients from subcutaneous, preperitoneal and visceral layers of adipose tissue (Fig.…”