SEM–EDS probing of morphological and physiological changes produced by a porphyrin photosensitizer in Psammobatis extenta electrocytes

Figueroa, María Prado; Santiago, Julio

doi:10.1016/j.micron.2006.08.004

Cited by 5 publications

(2 citation statements)

References 24 publications

(22 reference statements)

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“…This approach is usually performed in studies related to morphology and elemental analysis of surface materials 39 and was employed in this study due to the facility of carrying out elemental analysis of small sample amounts without sample preparation.…”

Section: Sem-eds Resultsmentioning

confidence: 99%

Alkaline Solubilization of Chicken Tissues Monitored by Raman Spectroscopy Followed by Pb Determination by GF AAS

Campos¹,

Oliveira²,

Izumi³

et al. 2017

J. Braz. Chem. Soc.

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This paper proposes a method for chicken tissues preparation using tetramethylammonium hydroxide (TMAH) for Pb determination by graphite furnace atomic absorption spectrometry employing multivariate approaches. Combining Raman spectroscopy with scanning electron microscopy coupled to energy-dispersive X-ray spectroscopy to monitor the solubilization process it was possible to show that TMAH acts in the solubilization of proteins, amino acids and lipids. Besides, this investigation showed that the small residual masses of samples have organic and inorganic compounds. Accuracy tests indicated that such residues have not interfered in the analytical results (recovery between 90-95%). The obtained limits of detection (0.099 µg g -1 ) and quantification (0.33 µg g -1 ) are compatible with the detectability required for regulatory purposes. Linearity (R 2 = 0.9925) and characteristic mass (13 pg) were also reported. Considering the set of 15 samples comercialized in Brazil, any Pb contamination was successfully observed and this was confirmed by the analysis of digested samples.

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Section: Sem-eds Resultsmentioning

confidence: 99%

Alkaline Solubilization of Chicken Tissues Monitored by Raman Spectroscopy Followed by Pb Determination by GF AAS

Campos¹,

Oliveira²,

Izumi³

et al. 2017

J. Braz. Chem. Soc.

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show abstract

“…Atomic force microscopy (AFM) and scanning electron microscopy (SEM) are the most common measures applied to describe their morphology [7][8][9]. Among them, SEM can be used to observe the morphological variety of DNA [7], proteins [10], and cells [9,11] due to the interaction with some small or large molecule substrates. In order to obtain information on DNA morphology, such as changes in the size and shape after the photo irradiation of amidinophenylporphyrins-DNA, the interactions between the porphyrins and DNA were investigated further by SEM.…”

Section: Introductionmentioning

confidence: 99%

Morphological characterization of amidinophenylporphyrins interacting with DNA by photo irradiation

Wang

Song

Zheng

et al. 2013

Chinese Chemical Letters

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Sublethal effects of pulp and paper mill effluent on two commonly cultured carps: a SEM- and EDS-based hematological biomarker analysis

Dey

Choudhury

Das

2016

Fish Physiol Biochem

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Blood being a vehicle for the transport of industrial pollutants in living system, fish hematology is considered as potent biomarker. In the present study, we investigated respective sublethal effects of pulp and paper mill effluents on hematology of two commonly cultured carps, Cyprinus carpio and Ctenopharyngodon idella, using optical, scanning electron microscopy and energy-dispersive X-ray spectroscopy. Irrespective of species, results showed significant decrease in erythrocyte, hematocrit and hemoglobin contents while an increase in white blood cell counts (P < 0.05). We observed an increasing trend of MCV (170.0 ± 3.07 to 193.16 ± 2.5) and MCH (34.31 ± 1.89 to 38.71 ± 3.61) up to 28th day in C. carpio (P < 0.05), while, in C. idella, the highest percent increase in MCV (180.8 ± 2.19) and MCH (32.9 ± 0.62) was observed on seventh exposure day, which subsequently declined, respectively, to 173.1 ± 17.1 and 27.9 ± 2.45 on 28th day. Unlike C. carpio, significant and progressive MCHC declining trend (18.23 ± 0.28 to 16.13 ± 0.31) was observed in C. idella. The most commonly observed abnormalities under SEM include echinocytes, cytoplasmic blebbing, cytoplasmic ring, spherocytes, lobopodial projections and acanthocytes in red blood cells of exposed fishes. EDS further revealed the presence of aluminum, antimony, arsenic, cadmium, mercury, tungsten, zinc and titanium; some of these metals were not even detected in the effluent samples, suggesting the probable metal bio-concentration in fish tissue, and subsequent jeopardization is a major concern particularly in the industrial area. Our study further suggested the use of sensitive and specific techniques like SEM and EDS in fish hematological biomarker analysis along with the conventional approach.

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SEM–EDS probing of morphological and physiological changes produced by a porphyrin photosensitizer in Psammobatis extenta electrocytes

Cited by 5 publications

References 24 publications

Alkaline Solubilization of Chicken Tissues Monitored by Raman Spectroscopy Followed by Pb Determination by GF AAS

Alkaline Solubilization of Chicken Tissues Monitored by Raman Spectroscopy Followed by Pb Determination by GF AAS

Morphological characterization of amidinophenylporphyrins interacting with DNA by photo irradiation

Sublethal effects of pulp and paper mill effluent on two commonly cultured carps: a SEM- and EDS-based hematological biomarker analysis

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