SEM and qRT-PCR revealed quercetin inhibits morphogenesis of <i>Aspergillus flavus</i> conidia via modulating calcineurin-Crz1 signalling pathway

Shishodia, Sonia Kumari; Tiwari, Shraddha; Hoda, Shanu; Vijayaraghavan, Pooja; Shankar, Jata

doi:10.1080/21501203.2020.1711826

Cited by 16 publications

(9 citation statements)

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“…Higher levels of calcium kinase, cAMP, Rhogdp, Plc and Pkc encoding genes were observed in groups treated with quercetin. Thus, the data suggested the antifungal potential of quercetin [ 29 ].…”

Section: Properties Of Quercetinmentioning

confidence: 99%

An insight into anticancer, antioxidant, antimicrobial, antidiabetic and anti-inflammatory effects of quercetin: a review

et al. 2022

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Flavonoids are present naturally in many fruits and vegetables including onions, apples, tea, cabbage, cauliflower, berries and nuts which provide us with quercetin, a powerful natural antioxidant and cytotoxic compound. Due to antioxidant property, many nutraceuticals and cosmeceuticals products contain quercetin as a major ingredient nowadays. Current review enlightened sources and quercetin’s role as an antioxidant, antimicrobial, antidiabetic, anticancerous and anti-inflammatory agent in medical field during last 5 to 6 years. Literature search was systematically done using scientific for the published articles of quercetin. A total of 345 articles were reviewed, and it was observed that more than 40% of articles were about quercetin’s use as an antioxidant agent, more than 25% of studies were about its use as an anticancer agent, and articles on antimicrobial activity were more than 15%. 10% of the articles showed anti-inflamamatory effects of quercetin. Literature search also revealed that quercetin alone and its complexes with chitosan, metal ions and polymers possessed good antidiabetic properties. Thus, the review focuses on new therapeutic interventions and drug delivery system of quercetin in medical field for the benefit of mankind.

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Section: Properties Of Quercetinmentioning

confidence: 99%

An insight into anticancer, antioxidant, antimicrobial, antidiabetic and anti-inflammatory effects of quercetin: a review

et al. 2022

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“…The hyaline conidial heads of A. flavus and globose to sub‐globose vesicles with conidia was also observed by Zakaria, (2018) using SEM. Another study by Shishodia, Tiwari, Hoda, Vijayaraghavan, & Shankar, (2020) also reported microscopic features of A. flavus using SEM analysis. The morphological features of A. flavus were also observed by Li et al (2022) in their study.…”

Section: Discussionmentioning

confidence: 95%

Light and scanning electron microscopic characterization of aflatoxins producing Aspergillus flavus in the maize crop

Seerat

Akram

Qureshi

et al. 2022

Microscopy Res & Technique

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Maize (Zea mays L.) is considered as one of the main cereals, used as a source of food, forage, and processed products. The loss of maize productivity is reported due to effect on roots, stalks, ears, and kernels mainly caused by many fungi. Among these fungal pathogens of maize, Aspergillus flavus (A. flavus) are the most prevalent that produces highly toxigenic aflatoxins that are highly carcinogenic to the consumers.The present study is confined to isolate and characterize the A. flavus from maize seeds for accurate identification that can be helpful for determination and management of aflatoxins in maize crop. Eighty stored seed samples of maize were collected from warehouses where seeds are stored for food and feeding purposes. For the isolation of A. flavus, Potato Dextrose Agar was used. Isolated fungi were identified macro and microscopically using light microscope and scanning electron microscope.A total of 212 Aspergillus isolates were identified based on macro-morphological and micro-morphological characteristics. The results showed that A. flavus colonies were granular, flat with yellow-green to deep yellow-green colony color having a white border and compact, spherical spore heads. Rapid rate of growth was observed maturing in about 3-5 days. In microscopic features, A. flavus have apically swollen conidiophores with various conidia bearing cells in long and dry chains. Spherical conidial heads were split into several columns ranging 300-400 μm in diameter. This will be helpful for farmers, researchers and traders in future for correct identification of sources of aflatoxins. Research Highlights• Maize seed samples were collected from Pothohar region of Pakistan.• The fungi were isolated on PDA.• Aspergillus flavus was identified macro-morphologically by observing growth rate, colony color and texture.• Furthermore, these fungi were identified micro-morphologically by using light and scanning electron microscope.• The 212 Aspergillus flavus strains were isolated and identified.

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“…In A. parasiticus , not only is crzA important in elevated calcium levels tolerance, but it also performs an essential function in aflatoxin biosynthesis, as deleted crzA mutants have a shallow ability (if any) to produce the toxin [ 79 ]. In A. flavus , it was reported that quercetin upregulates both calcineurin and crz1/crzA genes, upregulation that was interpreted as a response to quercetin-induced stress [ 80 ]. In A. niger , CrzA deletion results in lower biofilm formation, decreased adhesion and hydrophobicity of conidia, decreased hyphae flocculation ability, and altered cell wall integrity of the hyphae [ 53 ].…”

Section: Other Targets From the Calcineurin Pathwaymentioning

confidence: 99%

Potential Antifungal Targets for Aspergillus sp. from the Calcineurin and Heat Shock Protein Pathways

Ancuceanu

Hovaneț²,

Maria³

et al. 2022

IJMS

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Aspergillus species, especially A. fumigatus, and to a lesser extent others (A. flavus, A. niger, A. terreus), although rarely pathogenic to healthy humans, can be very aggressive to immunocompromised patients (they are opportunistic pathogens). Although survival rates for such infections have improved in recent decades following the introduction of azole derivatives, they remain a clinical challenge. The fact that current antifungals act as fungistatic rather than fungicide, that they have limited safety, and that resistance is becoming increasingly common make the need for new, more effective, and safer therapies to become more acute. Over the last decades, knowledge about the molecular biology of A. fumigatus and other Aspergillus species, and particularly of calcineurin, Hsp90, and their signaling pathway proteins, has progressed remarkably. Although calcineurin has attracted much interest, its adverse effects, particularly its immunosuppressive effects, make it less attractive than it might at first appear. The situation is not very different for Hsp90. Other proteins from their signaling pathways, such as protein kinases phosphorylating the four SPRR serine residues, CrzA, rcnA, pmcA-pmcC (particularly pmcC), rfeF, BAR adapter protein(s), the phkB histidine kinase, sskB MAP kinase kinase, zfpA, htfA, ctfA, SwoH (nucleoside diphosphate kinase), CchA, MidA, FKBP12, the K27 lysine position from Hsp90, PkcA, MpkA, RlmA, brlA, abaA, wetA, other heat shock proteins (Hsp70, Hsp40, Hsp12) currently appear promising and deserve further investigation as potential targets for antifungal drug development.

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SEM and qRT-PCR revealed quercetin inhibits morphogenesis of Aspergillus flavus conidia via modulating calcineurin-Crz1 signalling pathway

Cited by 16 publications

References 46 publications

An insight into anticancer, antioxidant, antimicrobial, antidiabetic and anti-inflammatory effects of quercetin: a review

An insight into anticancer, antioxidant, antimicrobial, antidiabetic and anti-inflammatory effects of quercetin: a review

Light and scanning electron microscopic characterization of aflatoxins producing Aspergillus flavus in the maize crop

Potential Antifungal Targets for Aspergillus sp. from the Calcineurin and Heat Shock Protein Pathways

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