Self-supporting electrospun PHB and PHBV/organoclay nanocomposite fibrous scaffolds

Ublekov, Filip; Budurova, Dessislava; Staneva, Maya; Natova, Margarita; Penchev, Hristo

doi:10.1016/j.matlet.2018.02.056

Cited by 17 publications

(8 citation statements)

References 14 publications

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“…This decreased the overall mechanical performance of PHBV/nCeO 2 nanocomposite membranes with higher filler loading. Our results were in agreement with those reported in the case of PHBV/clay nanocomposites …”

Section: Resultssupporting

confidence: 93%

“…Our results were in agreement with those reported in the case of PHBV/clay nanocomposites. 65 PHBV/nCeO 2 Membranes Enhance Cell Viability and Adhesion of HOEC and HMEC. To understand the effect of the nCeO 2 loaded in the membranes on human cell adhesion, we cultured HOEC and HMEC on the membranes and determined the cell adhesion behavior.…”

Section: ■ Results and Discussionmentioning

confidence: 97%

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Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications

Augustine

Hasan

Patan

et al. 2019

ACS Biomater. Sci. Eng.

142

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Insufficient cell proliferation, cell migration, and angiogenesis are among the major causes for nonhealing of chronic diabetic wounds. Incorporation of cerium oxide nanoparticles (nCeO2) in wound dressings can be a promising approach to promote angiogenesis and healing of diabetic wounds. In this paper, we report the development of a novel nCeO2 containing electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) membrane for diabetic wound healing applications. In vitro cell adhesion studies, chicken embryo angiogenesis assay, and in vivo diabetic wound healing studies were performed to assess the cell proliferation, angiogenesis, and wound healing potential of the developed membranes. The experimental results showed that nCeO2 containing PHBV membranes can promote cell proliferation and cell adhesion when used as wound dressings. For less than 1% w/w of nCeO2 content, human mammary epithelial cells (HMEC) were adhered parallel to the individual fibers of PHBV. For higher than 1% w/w of nCeO2 content, cells started to flatten and spread over the fibers. In ovo angiogenic assay showed the ability of nCeO2 incorporated PHBV membranes to enhance blood vessel formation. In vivo wound healing study in diabetic rats confirmed the wound healing potential of nCeO2 incorporated PHBV membranes. The study suggests that nCeO2 incorporated PHBV membranes have strong potential to be used as wound dressings to enhance cell proliferation and vascularization and promote the healing of diabetic wounds.

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Section: Resultssupporting

confidence: 93%

Section: ■ Results and Discussionmentioning

confidence: 97%

Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications

Augustine

Hasan

Patan

et al. 2019

ACS Biomater. Sci. Eng.

142

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“…In this regard, poly(3-hydroxybutyrate) (PHB) is an interesting biodegradable and biocompatible biopolymer synthesized by bacterial fermentation, but its crystalline properties hinder its promising rheological behavior as a biomaterial [ 9 ]. The use of PHB as a biocompatible material can be improved by induction of amorphization of its structure with the introduction of other biodegradable polymers [ 4 , 10 ]. Among them, poly(ethylene glycol) (PEG) and sodium alginate (ALG) are hydrophilic and amorphous biodegradable biopolymers widely used as additives for the fabrication of blends with improved mechanical properties for biomedical purposes [ 8 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Exploiting the Physicochemical and Antimicrobial Properties of PHB/PEG and PHB/PEG/ALG-e Blends Loaded with Ag Nanoparticles

et al. 2022

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Poly(3-hydroxybutyrate) (PHB)-based films containing Poly(ethylene glycol) (PEG), esterified sodium alginate (ALG-e) and polymeric additives loaded with Ag nanoparticles (AgNPs) were obtained by a conventional casting method. AgNPs were produced in aqueous suspension and added to polymeric gels using a phase exchange technique. Composite formation was confirmed by finding the Ag peak in the XRD pattern of PHB. The morphological analysis showed that the inclusion of PEG polymer caused the occurrence of pores over the film surface, which were overshadowed by the addition of ALG-e polymer. The PHB functional groups were dominating the FTIR spectrum, whose bands associated with the crystalline and amorphous regions increased after the addition of PEG and ALG-e polymers. Thermal analysis of the films revealed a decrease in the degradation temperature of PHB containing PEG/AgNPs and PEG/ALG-e/AgNPs, suggesting a catalytic effect. The PHB/PEG/ALG-e/AgNPs film combined the best properties of water vapor permeability and hydrophilicity of the different polymers used. All samples showed good antimicrobial activity in vitro, with the greater inhibitory halo observed for the PEG/PEG/AgNPs against Gram positive S. aureus microorganisms. Thus, the PHB/PEG/ALG-e/AgNPs composite demonstrated here is a promising candidate for skin wound healing treatment.

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“…Biopolymer based nanocomposites showed improved mechanical strength, in‐vitro degradation, biocompatibility, and bioactivity properties due to the addition of nanosized clay in the biopolymer matrix. The bio‐nanocomposites such as PHB/nano‐bioglass (nBG), PHB/octadecylamine‐modified montmorillonite (C18MMT), Ca–P/PHB, PHB/OMMT, PHB/chitosan/alumina nanowires, nHA/PHB, and PHB/nano silica bio‐nanocomposites were reported for improvement in properties such as surface roughness, protein adsorption, wettability, water uptake, degradation, thermal stability, bioresorbability, bioactivity, cell attachment, and cell proliferation 1,6,17–21 …”

Section: Introductionmentioning

confidence: 99%

Fabrication and properties of polyhydroxybutyrate/kaolin nanocomposites and evaluation of their biocompatibility for biomedical applications

Ankush

Pugazhenthi

Vasanth

2021

J of Applied Polymer Sci

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The polyhydroxybutyrate biopolymer nanocomposites (C1–C10) were fabricated by solvent casting method with different loading of kaolin and polyethylene glycol. Scanning electron microscopy showed that the microstructure of the composites varied with different kaolin loading. X‐ray diffraction and Fourier transform infrared spectroscopy analysis confirm the presence of kaolin in the polymer matrix due to the intercalation and formation of hydrogen bond. The contact angle of the nanocomposites decreased with increasing kaolin loading indicating an improvement in wettability of the nanocomposites. Thermogravimetric and differential scanning calorimetry analysis showed that the Tmax and Tm of the nanocomposites increased with increasing kaolin loading. The mechanical property of the nanocomposite fabricated with 10 wt% kaolin (C10) was found to have identical mechanical property with natural bone that was selected as an optimum nanocomposite. The nanocomposite showed prolonged blood clotting time exhibiting anticoagulant nature of the nanocomposite. Moreover, low protein adsorption (168 ± 8 μg/cm2), suppressed platelet adhesion (75 ± 2 × 109 platelets/cm2) and less complement activation (118 ± 5 mg/dl for C3 and 658 ± 5 mg/dl for C4) showed the improvement in surface properties of the nanocomposite. In vitro bioactivity studies revealed the formation of hydroxyapatite layer on the surface of the nanocomposites. Eventually, the nanocomposites (C10) showed no cytotoxic effect on MG‐63 cells as tested through MTT assay and it is biologically safe.

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Self-supporting electrospun PHB and PHBV/organoclay nanocomposite fibrous scaffolds

Cited by 17 publications

References 14 publications

Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications

Cerium Oxide Nanoparticle Incorporated Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Membranes for Diabetic Wound Healing Applications

Exploiting the Physicochemical and Antimicrobial Properties of PHB/PEG and PHB/PEG/ALG-e Blends Loaded with Ag Nanoparticles

Fabrication and properties of polyhydroxybutyrate/kaolin nanocomposites and evaluation of their biocompatibility for biomedical applications

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