Self-Renewable Tag for Photostable Fluorescence Imaging of Proteins

Du, Zhichao; Wang, Wenjing; Luo, Siyuan; Zhang, Lingjie; Yuan, Shuowei; Hei, Yongzhen; Bao, Zhangbin; Chen, Chunlai; Lin, Yi; Chu, Ling

doi:10.1021/jacs.3c06102

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…In 2023, Chu and co-workers introduced a self-renewable Tag (srTAG) based on mutant FKBP (F36L) protein for stable fluorescence imaging of proteins . They combined a small-molecule FKBP ligand AP1867 with the Janelia Fluor (JF) fluorophore to develop ZCD series chemical probes (Figure ).…”

Section: Protein Labeling Methods For Hybrid Small-molecule/protein P...mentioning

confidence: 99%

“…99 In 2023, Chu and co-workers introduced a self-renewable Tag (srTAG) based on mutant FKBP (F36L) protein for stable fluorescence imaging of proteins. 100 They combined a small-molecule FKBP ligand AP1867 with the Janelia Fluor (JF) fluorophore to develop ZCD series chemical probes (Figure 13). The ZCD-1 probe (41a) showed fluorogenic labeling of intracellular proteins and fluorescence recovery after photobleaching by self-renewal of the probe.…”

Section: Mutant Fkbp12mentioning

confidence: 99%

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Hybrid Small-Molecule/Protein Fluorescent Probes

Minoshima,

Reja,

Hashimoto

et al. 2024

Chem. Rev.

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Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein localization and function in living cells. These hybrid probes are constructed by diverse sitespecific chemical protein labeling approaches through chemical reactions to exogenous peptide/ small protein tags, enzymatic post-translational modifications, bioorthogonal reactions for genetically incorporated unnatural amino acids, and ligand-directed chemical reactions. The hybrid smallmolecule/protein fluorescent probes are employed for imaging protein trafficking, conformational changes, and bioanalytes surrounding proteins. In addition, fluorescent hybrid probes facilitate visualization of protein dynamics at the single-molecule level and the defined structure with superresolution imaging. In this review, we discuss development and the bioimaging applications of fluorescent probes based on small-molecule/protein hybrids.

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Section: Protein Labeling Methods For Hybrid Small-molecule/protein P...mentioning

confidence: 99%

Section: Mutant Fkbp12mentioning

confidence: 99%

Hybrid Small-Molecule/Protein Fluorescent Probes

Minoshima,

Reja,

Hashimoto

et al. 2024

Chem. Rev.

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Rna Buffering Fluorogenic Probe for Nucleolar Morphology Stable Imaging And Nucleolar Stress‐Generating Agents Screening

Jiang,

Qiao,

Chen

et al. 2024

Advanced Science

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In the realm of cell research, membraneless organelles have become a subject of increasing interest. However, their ever‐changing and amorphous morphological characteristics have long presented a formidable challenge when it comes to studying their structure and function. In this paper, a fluorescent probe Nu‐AN is reported, which exhibits the remarkable capability to selectively bind to and visualize the nucleolus morphology, the largest membraneless organelle within the nucleus. Nu‐AN demonstrates a significant enhancement in fluorescence upon its selective binding to nucleolar RNA, due to the inhibited twisted intramolecular charge–transfer (TICT) and reduced hydrogen bonding with water. What sets Nu‐AN apart is its neutral charge and weak interaction with nucleolus RNA, enabling it to label the nucleolus selectively and reversibly. This not only reduces interference but also permits the replacement of photobleached probes with fresh ones outside the nucleolus, thereby preserving imaging photostability. By closely monitoring morphology‐specific changes in the nucleolus with this buffering fluorogenic probe, screenings for agents are conducted that induce nucleolar stress within living cells.

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Chemical Approaches to Optimize the Properties of Organic Fluorophores for Imaging and Sensing

Jiang,

Liu,

Liu

et al. 2023

Angewandte Chemie

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Organic fluorophores are indispensable tools in cells, tissue and in vivo imaging, and have enabled much progress in the wide range of biological and biomedical fields. However, many available dyes suffer from insufficient performances, such as short absorption and emission wavelength, low brightness, poor stability, small Stokes shift, and unsuitable permeability, restricting their application in advanced imaging technology and complex imaging. Over the past two decades, many efforts have been made to improve these performances of fluorophores. Starting with the luminescence principle of fluorophores, this review clarifies the mechanisms of the insufficient performance for traditional fluorophores to a certain extent, systematically summarizes the modified approaches of optimizing properties, highlights the typical applications of the improved fluorophores in imaging and sensing, and indicates existing problems and challenges in this area. This progress not only proves the significance of improving fluorophores properties, but also provide a theoretical guidance for the development of high‐performance fluorophores.

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Self-Renewable Tag for Photostable Fluorescence Imaging of Proteins

Cited by 5 publications

References 39 publications

Hybrid Small-Molecule/Protein Fluorescent Probes

Hybrid Small-Molecule/Protein Fluorescent Probes

Rna Buffering Fluorogenic Probe for Nucleolar Morphology Stable Imaging And Nucleolar Stress‐Generating Agents Screening

Chemical Approaches to Optimize the Properties of Organic Fluorophores for Imaging and Sensing

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