In recent years, more and more computer technology has been applied in industrial Internet, the vulnerability and security risk of industrial control systems are gradually increasing. network security issues have received much attention. To address the complex attacks and relatively poor deployable defense resources in the Industrial Internet, this paper combines industrial Internet attacks with signal game theory, proposes a moving target defense strategy selection method. The SDN-based industrial Internet attack and defense model is established, the core decision module and the moving target defense module are introduced to select and implement the best defense strategy respectively, so that the industrial Internet can achieve the best security defense strategy. The simulation results show that the moving target defense strategy selection method based on the signal game can effectively improve the defender's gain and achieve the best defense against network attacks under the industrial Internet.