Self-Patterning Silk Films for Cellular Coculture

Abstract

Silk produced from Bombyx mori as a natural polymer has gained tremendous attraction for biomedical applications. Tissue engineering applications require meticulous spatial control over cellular adhesion, which is conventionally acquired via lithographic techniques. Here, we report formation of structures using nanogels that are readily cross-linked in the presence of photoactive groups that form stable, biocompatible patterned system for cellular growth. This direct approach has the proficiency to commission structures with reduced processing steps for obtaining biocompatible surface favoring stem cell adhesion, differentiation, and subsequent coculture with an additional cell type in a spatially controlled manner.

Antibacterial and Cytocompatible Nanoengineered Silk-Based Materials for Orthopedic Implants and Tissue Engineering

“…In real situations, bacteria and cells coexist and surface adhesion becomes a race. In this case, cell proliferation on preinfected samples can mimic the conditions in vivo. − In our experiments, after the samples had been preinfected with S. aureus and E.…”

Spatiotemporal Control over Cell Proliferation and Differentiation for Tissue Engineering and Regenerative Medicine Applications Using Silk Fibroin Scaffolds

“…After 30 min, the cells start to attach on the nanogels regions, resulting in a patterned coculture (images are at 10× and 4×). Reprinted with permission from ref . Copyright 2018 American Chemical Society.…”

“…In a recent report, we patterned SFs with biocompatible nanoparticles for fast, local delivery of small molecules, which can detach the adhered cells to give a predetermined region devoid of cells, and this region was then filled with other type of cells. 135 This strategy allows development of a self-patterned surface where cells can be cocultured with spatial control (Figure 2). Using this strategy, we were able to achieve region specific adhesion and patterning at the micron scale for different cell types.…”

One-step fabrication of three-dimensional macropore copolymer-modified polycarbonate array by photo-crosslinking for protein immunoassay

“…These surfaces modied by BPcontaining polymers are widely used in biosensing, 20,21 coating, 22 composite material 23 and surface patterning. [24][25][26] Photo-crosslinking technology can solve the problem of difficult and tedious problems in the modication of polymer substrate surface, while the 3D structural surface provides the basis for excellent biodetection performance of biochips.…”