Self-organizing multicellular structures designed using synthetic biology

Tordoff, Jesse; Weiss, Ron

doi:10.1038/d41586-018-05564-5

Cited by 4 publications

(1 citation statement)

References 8 publications

(18 reference statements)

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“…These circuits comprised parts that recognized environmental inputs, effected a logical operation using transcriptional components and fed the results of this operation to a GFP-reporter to reveal these behaviors. These initial studies have since been followed by a slew of transcriptional and translation circuit designs such as RNA-based systems, quorum-sensing and sensory circuits [54,56,[59][60][61][62].…”

Section: Building Synthetic Biology Circuitsmentioning

confidence: 99%

Can thiol-based redox systems be utilized as parts for synthetic biology applications?

Pillay

John

2021

Redox Report

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Objectives Synthetic biology has emerged from molecular biology and engineering approaches and aims to develop novel, biologically-inspired systems for industrial and basic research applications ranging from biocomputing to drug production. Surprisingly, redoxin (thioredoxin, glutaredoxin, peroxiredoxin) and other thiol-based redox systems have not been widely utilized in many of these synthetic biology applications. Methods We reviewed thiol-based redox systems and the development of synthetic biology applications that have used thiol-dependent parts. Results The development of circuits to facilitate cytoplasmic disulfide bonding, biocomputing and the treatment of intestinal bowel disease are amongst the applications that have used thiol-based parts. We propose that genetically encoded redox sensors, thiol-based biomaterials and intracellular hydrogen peroxide generators may also be valuable components for synthetic biology applications. Discussion Thiol-based systems play multiple roles in cellular redox metabolism, antioxidant defense and signaling and could therefore offer a vast and diverse portfolio of components, parts and devices for synthetic biology applications. However, factors limiting the adoption of redoxin systems for synthetic biology applications include the orthogonality of thiol-based components, limitations in the methods to characterize thiol-based systems and an incomplete understanding of the design principles of these systems.

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