Self-organization of the human embryo in the absence of maternal tissues

Shahbazi, Marta; Jędrusik, Agnieszka; Vuoristo, Sanna; Recher, Gaëlle; Hupalowska, Anna; Bolton, Virginia N.; Fogarty, Norah M. E.; Campbell, Alison; Devito, Liani; Ilić, Duško; Khalaf, Yakoub; Niakan, Kathy K.; Fishel, Simon; Zernicka‐Goetz, Magdalena

doi:10.1038/ncb3347

Cited by 536 publications

(457 citation statements)

References 34 publications

Supporting

Mentioning

424

Contrasting

Unclassified

Order By: Relevance

“…As the primate embryo implants, a penetrating mass of invasive STB forms in the implantation zone and soon surrounds the conceptus. Such a syncytium also forms in human conceptuses cultured for 13 d or 14 d in vitro, when it forms the periphery of a layer of trophoblast encircling the epiblast (74,75). It has been inferred that this syncytial mass, as it advances within the uterine wall, hollows out lacunae, which become filled with fluid from breached uterine glands and maternal blood vessels and likely provision the conceptus (67,(76)(77)(78).…”

Section: Release Of Infection-competent Virus By Esc and Esc-derived mentioning

confidence: 99%

Vulnerability of primitive human placental trophoblast to Zika virus

Sheridan

Yunusov

Balaraman

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

155

View full text Add to dashboard Cite

Infection of pregnant women by Asian lineage strains of Zika virus (ZIKV) has been linked to brain abnormalities in their infants, yet it is uncertain when during pregnancy the human conceptus is most vulnerable to the virus. We have examined two models to study susceptibility of human placental trophoblast to ZIKV: cytotrophoblast and syncytiotrophoblast derived from placental villi at term and colonies of trophoblast differentiated from embryonic stem cells (ESC). The latter appear to be analogous to the primitive placenta formed during implantation. The cells from term placentas, which resist infection, do not express genes encoding most attachment factors implicated in ZIKV entry but do express many genes associated with antiviral defense. By contrast, the ESC-derived trophoblasts possess a wide range of attachment factors for ZIKV entry and lack components of a robust antiviral response system. These cells, particularly areas of syncytiotrophoblast within the colonies, quickly become infected, produce infectious virus and undergo lysis within 48 h after exposure to low titers (multiplicity of infection > 0.07) of an African lineage strain (MR766 Uganda: ZIKVU) considered to be benign with regards to effects on fetal development. Unexpectedly, lytic effects required significantly higher titers of the presumed more virulent FSS13025 Cambodia (ZIKVC). Our data suggest that the developing fetus might be most vulnerable to ZIKV early in the first trimester before a protective zone of mature villous trophoblast has been established. Additionally, MR766 is highly trophic toward primitive trophoblast, which may put the early conceptus of an infected mother at high risk for destruction.

show abstract

Section: Release Of Infection-competent Virus By Esc and Esc-derived mentioning

confidence: 99%

Vulnerability of primitive human placental trophoblast to Zika virus

Sheridan

Yunusov

Balaraman

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

155

View full text Add to dashboard Cite

show abstract

“…O n 4 May, two groups reported that they had sustained human embryos in vitro for 12-13 days [1][2][3] . Embryos normally implant in the wall of the uterus at around day seven.…”

Section: Geneticsmentioning

confidence: 99%

Embryology policy: Revisit the 14-day rule

Hyun

Wilkerson

Johnston

2016

Nature

151

105

View full text Add to dashboard Cite

show abstract

“…Several mammals, however, lack amniotic folds or have only rudimentary ones. In the rhesus monkey (Enders et al, 1986) and human (Shahbazi et al, 2016), the amnion forms by cavitation as a space appears amidst a group of polarized cells in the epiblast. In the mouse, a proamnion appears in the centre of the epiblast, but the true amnion is formed from the amniochorionic fold (Dobreva et al, 2010).…”

Section: Amniogenesismentioning

confidence: 99%

Advances in flow cytometry in basic and applied equine andrology

2017

View full text Add to dashboard Cite

Molecular phylogenetics has made a substantial contribution to our understanding of the relationships between mammalian orders and has generated trees that can be used to examine the evolution of anatomical and physiological traits. We here summarize findings on fetal membranes and placentation in Ferungulata, a clade comprising carnivores, pangolins, and even-and odd-toed ungulates. Their early ontogeny shows several conserved traits such as superficial attachment of the blastocyst, amnion formation by folding, a large allantoic sac and a temporary yolk sac placenta. In contrast, several characters of the chorioallantoic placenta are derived, including the diffuse and cotyledonary placental types in ungulates and zonary placenta in carnivores, specializations of the interhaemal barrier, the presence of areolae or haemophagous regions and lack of stromal decidual cells. Ungulates produce large amounts of placental proteins including placental lactogens and pregnancy-associated glycoproteins. Evolutionary innovations of the placental system may contribute to the high diversity of lifestyles within Ferungulata and be linked to the evolution of highly precocial offspring in ungulates.

show abstract

Self-organization of the human embryo in the absence of maternal tissues

Cited by 536 publications

References 34 publications

Vulnerability of primitive human placental trophoblast to Zika virus

Vulnerability of primitive human placental trophoblast to Zika virus

Embryology policy: Revisit the 14-day rule

Advances in flow cytometry in basic and applied equine andrology

Contact Info

Product

Resources

About