Self‐organization effects in reactions of macromolecules

Abstract

ABSTRACT:The ability of biomacromolecules to coordinate fast reaction events in their active centers with slow rearrangements of their structure is focused upon. Under real conditions of many sequential reaction turnovers of the macromolecule, the structural shifts caused by single turnovers can be of cumulative character. This, in turn, leads to drastic threshold-like changes of the reaction cycle. In this way, the creative role of dynamical substrate-conformation interactions in the formation of functional regimes of the macromolecule is consistently uncovered.

Indirect Evidences of Conformational Regulation in Protein Reactions: How Much Can Be Learnt?

“…Due to the presence of a stable optical marker -in particular, the bleaching of the absorption band at 865 nm indicated the donor photoexcitation [11,12], so that the normalized absorbance changes show the populations of reaction states PQ B and P + Q − B -the reaction kinetics can be traced to a high accuracy on a wide time scale by the optical spectroscopy methods; for the relevant setup, see, e.g., [21]. Typical experimental curves represent the time course of the initial charge photoseparation under the action of actinic light of certain intensity I and the exposure duration t exp with subsequent recombination (which can last from seconds to tens of minutes) after switching-off light [19,20,22,23].…”