Self-organization and neuronal avalanches in networks of dissociated cortical neurons

Pasquale, Valentina; Massobrio, Paolo; Bologna, Luca; Chiappalone, Michela; Martinoia, Sergio

doi:10.1016/j.neuroscience.2008.03.050

Cited by 365 publications

(416 citation statements)

References 64 publications

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“…[23] as being consistent with criticality. The expected power law distribution in the size of cortical avalanches and lifetime statistcs [59,60] are not straightforward to simulate in the present simulations, which do not include this dynamical behavior; in addition, further work is required to identify a measure of avalanche size that corresponds to that available from biological systems [59,60]. We speculate that the power law behavior discussed in Sec.…”

Section: F Avalanches and Emergent Complex Dynamicsmentioning

confidence: 91%

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Neuromorphic behavior in percolating nanoparticle films

Fostner

Brown

2015

Phys. Rev. E

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We show that the complex connectivity of percolating networks of nanoparticles provides a natural solid-state system in which bottom-up assembly provides a route to realization of neuromorphic behavior. Below the percolation threshold the networks comprise groups of particles separated by tunnel gaps; an applied voltage causes atomic scale wires to form in the gaps, and we show that the avalanche of switching events that occurs is similar to potentiation in biological neural systems. We characterize the level of potentiation in the percolating system as a function of the surface coverage of nanoparticles and other experimentally relevant variables, and compare our results with those from biological systems. The complex percolating structure and the electric field driven switching mechanism provide several potential advantages in comparison to previously reported solid-state neuromorphic systems.

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Section: F Avalanches and Emergent Complex Dynamicsmentioning

confidence: 91%

“…We speculate that the power law behavior discussed in Sec. III B 1 can be related to experimentally observed power laws for neuronal avalanche size and lifetime [59,60].…”

Section: F Avalanches and Emergent Complex Dynamicsmentioning

confidence: 94%

Neuromorphic behavior in percolating nanoparticle films

Fostner

Brown

2015

Phys. Rev. E

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“…In our laser stimulation experiments, we chose to focus on the time period between DIV 14 and DIV 21. This is before spontaneous bursting emerges in mass cultures (Pasquale et al, 2008;, and our wide-field experiments suggested that it should be possible to stimulate neurons during this time window.…”

Section: Laser Stimulationmentioning

confidence: 81%

“…Multi-electrode arrays (MEAs) have also been extensively used to probe cultured neural networks (Corner et al, 2002;Pasquale et al, 2008;Wagenaar et al, 2005), namely to characterize the bursting activity in developing cultured neural networks and control activity in these networks by stimulating at different electrode sites (Madhavan et al, 2006;Massobrio et al, 2007;Wagenaar et al, 2005). However, the extracellular stimulation from the array suffers from poor spatial localization (Heuschkel et al, 2002) and stimulation artifacts (Wagenaar and Potter, 2004).…”

Section: Discussionmentioning

confidence: 99%

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Laser-evoked synaptic transmission in cultured hippocampal neurons expressing channelrhodopsin-2 delivered by adeno-associated virus

Wang¹,

Hasan²,

Seung³

2009

Journal of Neuroscience Methods

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We present a method for studying synaptic transmission in mass cultures of dissociated hippocampal neurons based on patch clamp recording combined with laser stimulation of neurons expressing Channelrhodopsin-2 (ChR2). Our goal was to use the high spatial resolution of laser illumination to come as close as possible to the ideal of identifying monosynaptically coupled pairs of neurons, which is conventionally done using microisland rather than mass cultures. Using recombinant adenoassociated virus (rAAV) to deliver the ChR2 gene, we focused on the time period between 14 and 20 days in vitro, during which expression levels are high, and spontaneous bursting activity has not yet started. Stimulation by wide-field illumination is sufficient to make the majority of ChR2-expressing neurons spike. Stimulation with a laser spot at least 10 μm in diameter also produces action potentials, but in a reduced fraction of neurons. We studied synaptic transmission by voltageclamping a neuron with low expression of ChR2 and scanning a 40 μm laser spot at surrounding locations. Responses were observed to stimulation at a subset of locations in the culture, indicating spatial localization of stimulation. Pharmacological means were used to identify responses that were synaptic. Many responses were of smaller amplitude than those typically found in microisland cultures. We were unable to find an entirely reliable criterion for distinguishing between monosynaptic and polysynaptic responses. However, we propose that postsynaptic currents with Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HHMI Author ManuscriptHHMI Author Manuscript HHMI Author Manuscript small amplitudes, simple shapes, and latencies not much greater than 8 msec are reasonable candidates for monosynaptic interactions.

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Self‐Organized Criticality in Neural Network Models

Rybarsch

Bornholdt

2014

Criticality in Neural Systems

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Self-organization and neuronal avalanches in networks of dissociated cortical neurons

Cited by 365 publications

References 64 publications

Neuromorphic behavior in percolating nanoparticle films

Neuromorphic behavior in percolating nanoparticle films

Laser-evoked synaptic transmission in cultured hippocampal neurons expressing channelrhodopsin-2 delivered by adeno-associated virus

Self‐Organized Criticality in Neural Network Models

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