Self-organization and evolution of function and structure in cultured neuronal networks

Abstract

Cultured neuronal networks (CNNs) have recently achieved major relevance as an alternative to in vivo models. While many works investigate the evolution of functional connectivity alone, experimental evidence of the simultaneous change of the structural neuronal network substrate is scarce. In the present study, we monitored the coevolution of structural and functional connectivities of neuronal cultures grown on top of microelectrode arrays, in a setup that allows the simultaneous recording of electrophysiological signals and microphotography detailed to the single-link level. During the observed 3 weeks lifespan, initially isolated invertebrate neurons form an ex novo complex circuitry of neuronal aggregates characterized by a small-world topology, with abundant neuronal loops (high clustering) and short distances. At the same time, the observation of synchronization events among electrodes reaches a maximum, coinciding with the spatial percolation of the neuronal network. At this stage, the correlation between the structural and the corresponding functional network is the largest, with around 15% of the physical links relating electrodes that fire synchronously. As the culture matures, this correlation smoothly declines but becomes more significant. Finally, at the local level, we found that the electrodes supporting the most coherent activity, the functional hubs, are not hubs in the physical circuitry but nodes with an average degree. This study demonstrates that functional networks of self-organized neuronal systems are discreet proxies of the underlying structure. It paves the way for future investigations to elucidate the intricate relationship between structure and function in other scenarios mediated by external stimulation.