Self-induced nystatin resistance in Dictyostelium discoideum

Kasbekar, Durgadas P.; Madigan, Sanford J.; Katz, Eugene R.

doi:10.1128/aac.27.6.974

Cited by 7 publications

(4 citation statements)

References 13 publications

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“…We prefer an alternative model to account for the pisatin induction of nystatin resistance. This model is analogous to the one proposed earlier for the induction of nystatin resistance following growth on the azasterol A25822B (5). In the present model, growth on pisatin induces membrane alterations that allow the cells to survive longer in the presence of otherwise cytotoxic concentrations of nystatin and thereby predisposes them to acquire nystatin resistance.…”

Section: Methodssupporting

confidence: 73%

“…The nystatin resistance was indistinguishable from the transient nystatin resistance (induced, for example, by growth on the sterol biosynthesis " Amoebae were harvested either from pis150 SM agar plates or from pisatin-free SM agar plates and suspended in Bonner's salt solution. The EOP was calculated as described in Table 1, footnote b. inhibitor azasterol A25822B and maintained by continued growth on nys80) previously reported for D. discoideirn (5). pis150 medium-grown cells that had subsequently been passaged once on pisatin-free medium became as nystatin sensitive as cells that were maintained throughout the experiment on SM medium.…”

Section: Methodsmentioning

confidence: 99%

“…We have, for the following reasons, undertaken to examine the cellular slime mold Dictyostelium discoideum for inducible, nondegradative resistance to pisatin. (i) One of us has studied the induction of resistance in D. discoideum to the polyene antibiotic nystatin (4) and found that pretreatment with the sterol biosynthesis inhibitor azasterol A25822B permitted growth of the cells on a medium containing an otherwise inhibitory concentration of nystatin (5). After induction by the azasterol, the nystatin resistance phenotype could be maintained by the nystatin in the medium.…”

mentioning

confidence: 99%

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An inducible, nondegradative phytoalexin resistance mechanism in Dictyostelium discoideum is suppressed by mutations that alter membrane sterol composition

Kasbekar

Papavinasasundaram

1992

Appl Environ Microbiol

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Pretreatment of Dictyostelium discoideum amoebae with a sublethal concentration of the pea phytoalexin pisatin was shown to induce nondegradative resistance to subsequent challenges with inhibitory concentrations. An alteration of membrane sterol composition either with the azasterol A25822B or by mutations in nysC that confer resistance to the polyene antibiotic nystatin suppressed the induction of pisatin resistance. Wild-type cells grown on pisatin medium acquired resistance to nystatin; however, after transfer to nystatin medium, they lost their pisatin resistance phenotype but remained nystatin resistant. To account for this asymmetry in the induction and maintenance of cross-resistance after growth on pisatin and nystatin media, we propose a model in which the two resistance phenotypes are governed by distinct mechanisms. This model presumes that growth on pisatin induces membrane alterations that predispose cells to acquire nystatin resistance but that the pisatin-induced membrane alterations are not maintained in the absence of pisatin.

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Section: Methodssupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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An inducible, nondegradative phytoalexin resistance mechanism in Dictyostelium discoideum is suppressed by mutations that alter membrane sterol composition

Kasbekar

Papavinasasundaram

1992

Appl Environ Microbiol

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“…An azasterol resistant, diploid clone exhibiting wild-type morphogenetic behavior was purified and haploidized by treatment with ben late [18] and 45 nystatin resistant segregants were purified. Since Noc 320 has been found to suppress nystatin resistance [19], picking the nystatin resistant haploids insured that none would bear the Noc 320 allele. Their morphogenetic behavior at 27 "C and at 22 "C is summarized in Table I.…”

Section: Resultsmentioning

confidence: 99%

Coh A, a mutation affecting the post aggregative related (par) cohesive system of Dictyostelium discoideum

et al. 1985

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Three stage-specific cohesive systems operate in D. discoideum: VEG, elaborated by vegetative cells; AR, by aggregation competent cells; and PAR, by post aggregation stage cells. Previous study of a mutant strain JC-5 had shown the stability of its PAR system (but not the AR) to be temperature sensitive. However, the phenotypic expression of this mutation termed Coh A is complicated by the presence in that strain of a preexisting mutant gene Rde A, which accelerates developmental events generally and alters the pattern of morphogenesis. Genetic evidence presented here indicates that the two mutations have been separated by parasexual recombination yielding a Coh A, Rde A t segregant class of which strain JC-36 is a prototype.At the permissive temperature, JC-36 follows a morphogenetic sequence like that of the wild type in respect to timing, morphogenetic pattern, and spore appearance. At the restrictive temperature, it forms normal aggregates at the usual time but exhibits two morphogenetic aberrancies during post aggregative development. First, fruit construction is arrested at a stage approximating the 16 hr "Bottle" stage of the wild type, though more squat and blunt tipped, and then the aggregate regresses. Cytodifferentiation into spores and stalk cells is also blocked. Second, a shift of slugs migrating normally at the permissive temperature to the restrictive causes the latter to disintegrate progressively as they leave clumps of cells behind them within the flattened sheath.JC-36 cells developing at the restrictive temperature also exhibited a decrease in EDTA resistant cohesivity attributable on two grounds to the sensitivity of the PAR system. In addition, the disappearance of the AR system completed in the wild type by the Mexicanhat (18-19 hr) stage is indefinitely arrested at an intermediate level in

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Nondegradative pisatin-resistance inDictyostelium discoideum, Neurospora crassa andNectria haematococca: Similarities and differences

Kasbekar

1994

J Biosci

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Self-induced nystatin resistance in Dictyostelium discoideum

Cited by 7 publications

References 13 publications

An inducible, nondegradative phytoalexin resistance mechanism in Dictyostelium discoideum is suppressed by mutations that alter membrane sterol composition

An inducible, nondegradative phytoalexin resistance mechanism in Dictyostelium discoideum is suppressed by mutations that alter membrane sterol composition

Coh A, a mutation affecting the post aggregative related (par) cohesive system of Dictyostelium discoideum

Nondegradative pisatin-resistance inDictyostelium discoideum, Neurospora crassa andNectria haematococca: Similarities and differences

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