Self‐incompatibility in flowering plants

Dickinson, H. G.

doi:10.1002/bies.950120403

Cited by 29 publications

(10 citation statements)

References 18 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…36; C. Elleman, personal communication). Circumstantial evidence also points to a role in SI (1,7,17,30). The presence ofrelatively few polypeptides, many with unusual properties, in the coating suggests that some may also be involved in these processes.…”

Section: Discussionmentioning

confidence: 98%

“…The self-incompatibility (SI) systems of higher plants are emerging as a family of unique signaling systems evolved from mechanisms already operative in the pollen/pistil interaction (1). Thus, in Nicotiana and other members of the Solanaceae, self-pollen is rejected by a mechanism involving a stylar RNase (2,3), an enzyme common to the styles of many plants and hypothesized to play a role in defense against pathogens.…”

mentioning

confidence: 99%

“…Following self-pollinations, development is rapidly arrested by a biostatic mechanism requiring protein synthesis and glycosylation (15). The pollen coat is derived from the sporophytic tapetum (16) and the fact that the SLG promoter drives expression in this layer suggests that it may be the location of determinants involved in the male SI response (1,17). We report here the removal and partial characterization of the pollen grain coating and describe an interaction between a 7-kDa coating peptide and female S-linked and related glycoprotein.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Interaction between a coating-borne peptide of the Brassica pollen grain and stigmatic S (self-incompatibility)-locus-specific glycoproteins.

Doughty

Hedderson

McCubbin

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

139

145

View full text Add to dashboard Cite

Methods are described for the removal of the sporophytic pollen grain coating of Brassica olracea and for the isolation of coat polypeptides. The coat contains a small number of proteins ranging from 6 to 45 kDa. Many of the larger proteins are glycosylated, while all carry hig positive charges resulting in pI values from 8.5 to 11. Polypeptides with pI values of 9.5, 9.0, and 8.5 possess strong esterase activity. No major differences could be detected in either pI values or molecular masses of pollen-coating polypeptides from grains carrying different sporophytically expressed S (self-incompatibility) alleles. Mixing pollen coat proteins with smatic extracts results in a conspicuous binding interaction involving female S-locus-specific and perhaps S-locus-related glycoproteins. This interaction, which is reversed by heating in the presence of SDS, results in an apparent charge shift of the female glycoprotein(s) of up to 2 pI units. The male participant in this interaction has been isolated by using a combination of fast protein liquid chromatography and reverse-phase HPLC and was shown to be a 7-kDa nonglycosylated peptide. Experiments with whole pollen cultured in vitro show challenge with stigmatic extracts to stimulate the release of gametophytic and sporophytic polypeptides and to result in the formation of a conspicuous interaction product, demonstrating the 7-kDa peptide to be freely available within the coating of pollen in vivo.The self-incompatibility (SI) systems of higher plants are emerging as a family of unique signaling systems evolved from mechanisms already operative in the pollen/pistil interaction (1). Thus, in Nicotiana and other members of the Solanaceae, self-pollen is rejected by a mechanism involving a stylar RNase (2, 3), an enzyme common to the styles of many plants and hypothesized to play a role in defense against pathogens. In most species, SI is regulated by a simple genetic system based on few loci and large numbers of alleles (4, 5); generally, if pollen and stigma carry alleles in common, the pollen/stigma interaction is disrupted. The genetics of SI in Brassica and other members of the Cruciferae and Compositae is not so straightforward; the pollen S phenotype is determined by the S-allelic constitution of the parent plant, rather than that of the haploid grain (6).Through a series of elegant molecular studies, Nasrallah et al. (7) have demonstrated two types of sequence to be linked to the S locus of Brassica. One (S locus glycoprotein; SLG) encodes a 55-kDa glycoprotein (S-locus-specific glycoprotein; SLSG) expressed in the stigmatic papillae, and the other encodes a transmembrane kinase (S receptor kinase; SRK) expressed in the organs ofboth sexes. Sequence comparisons suggest that the SRK features a domain, which may be extracellular, with maintained homology to the SLG. Further, other gene families with considerable homology to the SLG are also present in the genome (8-10) but unlinked to the S locus (S locus related; SLR). Interestingly, reporter constructs driven...

show abstract

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Interaction between a coating-borne peptide of the Brassica pollen grain and stigmatic S (self-incompatibility)-locus-specific glycoproteins.

Doughty

Hedderson

McCubbin

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

139

145

View full text Add to dashboard Cite

show abstract

“…It has long been considered that the male self-incompatibility determinants are carried in tbe coating, and we would now suggest that certain 'compatibility' factors are also contained in this layer, which are essential for the normal germination and penetration of a B. oleracea stigma by B. oleracea pollen. The putative dual role of the S-linked and Srelated glycoproteins, some of wbich are apparently held in the coating, is discussed in detail elsewhere (Dickinson, 1989).…”

Section: Lilscussionmentioning

confidence: 99%

The role of the exine coating in pollen–stigma interactions in Brassica oleracea L

Elleman

Dickinson

1990

New Phytologist

View full text Add to dashboard Cite

SUMMARYWhen the dehydrated pollen grain of Brassica oleracea L. alights on a receptive stigma the pollen coat flows out from the exine to form an appresoria-like ' foot' and, within a matter of some 30 min, gross ultrastructural changes become visible both within the protoplast and in the foot itself. These changes are interpreted as reflecting the limited movement of water, and presumably other materials, from the stigma to the grain. The compatible pollen grain then continues to take up water, whilst undergoing other cytoplasmic changes and eventually producing the pollen tube. The tube grows from the colpus towards the point of contact with the stigma, beneath which the outer layer of the papillar wall has become more loosely packed. The pollen tube enters the wall at this point and, as a consequence of its rapid extension, the grain is frequently lifted away from the papilla. The tube then grows between two layers of the pectocellulosic papillar wall into the stigmatic parenchyma, where it follows an intercellular route. These events are discussed in terms of current views of the relationship between male and female cells at these early stages of the pollen stigma interaction.

show abstract

“…Many flowering plants possess genetically controlled selfincompatibility (Sl) systems that prevent inbreeding (for reviews, seedeNettancourt, 1977; Dickinson, 1990;Haringetal., 1990). Sl is often controlled by a single, multiallelic "S"-locus.…”

Section: Introductionmentioning

confidence: 99%

Expression of a Self-Incompatibility Glycoprotein (S 2 -Ribonuclease) from Nicotiana alata in Transgenic Nicotiana tabacum

Murfett¹,

Cornish²,

Ebert³

et al. 1992

The Plant Cell

View full text Add to dashboard Cite

In Nicotiana alata, self-incompatibility is controlled by a single locus, designated the S-locus, with multiple alleles. Stylar products of these alleles are ribonucleases that are secreted mainly in the transmitting tract tissues. N. tabacum plants were transformed with constructs containing the S2-cDNA and genomic S2-sequences from N. alata that were linked to the cauliflower mosaic virus 35S promoter. Unlike other genes controlled by this promoter, the genes were expressed most highly in mature floral organs. This pattern of expression was observed at both the protein and RNA levels. The S2-glycoprotein was detected in the stylar transmitting tract tissues of the transgenic plants. The transgene product was secreted, had ribonuclease activity, and was glycosylated with the correct number of glycan chains. However, the maximum level of S2-glycoprotein in styles of the transgenic plants was approximately 100-fold lower than that found in N. alata styles carrying the S2-allele. Perhaps because of this lower protein level, the plants showed no changes in the incompatibility phenotype.

show abstract

Self‐incompatibility in flowering plants

Cited by 29 publications

References 18 publications

Interaction between a coating-borne peptide of the Brassica pollen grain and stigmatic S (self-incompatibility)-locus-specific glycoproteins.

Interaction between a coating-borne peptide of the Brassica pollen grain and stigmatic S (self-incompatibility)-locus-specific glycoproteins.

The role of the exine coating in pollen–stigma interactions in Brassica oleracea L

Expression of a Self-Incompatibility Glycoprotein (S 2 -Ribonuclease) from Nicotiana alata in Transgenic Nicotiana tabacum

Contact Info

Product

Resources

About