Self-(in)compatibility of the almonds P. dulcis and P. webbii: detection and cloning of ‘wild-type S f ’ and new self-compatibility alleles encoding inactive S-RNases

Bošković, R.; Tobutt, K. R.; Ortega, Elisa Martín; Sutherland, B. G.; Godini, A.

doi:10.1007/s00438-007-0283-4

Cited by 45 publications

(62 citation statements)

References 35 publications

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“…PCR-based markers of almond self incompatibility S -alleles have been successfully used to identify different self-incompatibility genotypes (Barckley et al 2006 ;Bošković et al 2007 ;Channuntapipat et al 2003 ;López et al 2004 ;Ortega et al 2005 ;Tamura et al 2000 ) , and to identify more than 30 different S -RNases (Kodad et al , 2010Barckley et al 2006 ;Ortega et al 2006 ) . Similar results were obtained by Bošković et al ( 2003Bošković et al ( , 2007 who identifi ed major almond cultivar stylar S -RNases by electrophoresis in vertical polyacrylamide gels.…”

Section: Integration Of New Biotechnologiesmentioning

confidence: 99%

Almond

Alonso¹,

Kodad

Gradziel

2011

Fruit Breeding

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The almond is economically the most important tree nut in the world. Its production is limited to areas characterized by a Mediterranean climate, including regions in the Mediterranean countries, the Central Valley of California, the Middle East, Central Asia, the Himalayan slopes, and the Southern Hemisphere, including Chile, Argentina, South Africa, and Australia. The main production region in the world is the Central Valley of California. The cultivation of almond in the eastern Mediterranean area occurred as early as the second millennium BC. Selection for domesticated almond types favored sweet kernels and larger nut size among these wild populations. Traditional seed propagation resulted in extensive genetic variability due, in part, to the obligate out-crossing nature of the self-incompatible almond. Local cultivars and landraces were selected over centuries of almond growing and in the twentieth century breeding activities began. Currently, there is active almond breeding programs in Spain, France, the USA and Israel. Self-compatibility has become the main objective along with late blooming, frost tolerance, resistance to diseases, and tree architecture. Despite the diffi culties in defi ning a kernel quality ideotype due to the differences in consumer preferences, almond quality is currently an important breeding goal.

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Section: Integration Of New Biotechnologiesmentioning

confidence: 99%

Almond

Alonso¹,

Kodad

Gradziel

2011

Fruit Breeding

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“…A més, fa poc que Bošković et al (2007) han identificat una S-RNasa idèntica a la S fa -RNasa d'aquestes quatre cultivars a d'altres tres cultivars autoincompatibles de la mateixa regió italiana que 'Tuono', la Pulla, que són 'Fra Giulio Grande', 'Santoro' i 'Cinquanta Vignali'. Això pot indicar que les poblacions d'ametller d'aquestes dues regions mediterrànies podrien estar relacionades i fins i tot es podria sospitar un possible intercanvi de material, no sols en el remot passat (Socias i Company, 1988), sinó també en èpoques més properes, com indica el conreu a Mallorca d'una cultivar com 'Sicilià' que es considera procedent d'aquesta altra illa mediterrània.…”

Section: Correlacions Al·lèliques I Geogràfiquesunclassified

“…del centre d'Espanya (Sán-chez & Oliveira, 2005). Igualment Kodad et al (2008) han assenyalat que els al·lels S 1 , S 33 i S f de l'ametller eren idèntics als S 6 , S 8 i S f identificats a diferents ecotips de P. webbii (Sánchez & Oliveira, 2005;Bošković et al, 2007). Tots aquests resultats suporten la hipòtesi de la possibilitat d'introgressió d'aquests al·lels per mitjà d'hibridació des de P. webbii a l'ametller (Socias i Company, 2004), o que aquests al·lels s'hagin pogut heretar des d'un avantpassat comú (Kodad et al, 2008).…”

Section: Correlacions Al·lèliques I Geogràfiquesunclassified

“…El fet que S f sigui inactiu als genotips autocompatibles, tal com s'ha observat a 'Tuono' i a 'Cambra' (Bošković et al, 2007;Kodad et al, 2009b), però que sigui actiu a genotips autoincompatibles, com són aquestes quatre cultivars mallorquines, encara no s'ha pogut explicar. S'han proposat diverses hipòtesis per a explicar aquest fet, tal com una mutació que afecti qualque fragment fora de la regió codificant (Kodad et al, 2009b).…”

Section: Correlacions Al·lèliques I Geogràfiquesunclassified

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Peculiaritats al·lèliques del locus <em>S</em> de les cultivars mallorquines d’ametller

2013

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AllElIC PECulIARITIES OF ThE S lOCuS OF ThE AlMOND CulTIvARS FROM MAjORCA.-The examination of the allelic diversity of the locus S in ten almond cultivars from Majorca has shown the presence of the S f allele in four of them, as well as the presence of five new alleles non identified so far. Although the S f allele has been described as responsible for the expression of self-compatibility in almond, it has been recently shown that this allele has two different phenotypic expressions. As a consequence, this allele cannot be always considered an index for self-compatibility of the genotypes harbouring it. The study of the four Majorcan cultivars possessing this allele has confirmed their selfincompatibility, showing that this allele is in its phenotypically active form, as it has been also observed in a group of cultivars from southern Italy, a fact possibly suggesting a connexion between these two Mediterranean almond populations.Key words: alleles; almond; Prunus amydgalus; S locus; self-compatibility. ResumenPECulIARIDADES AlélICAS DEl lOCuS S DE lOS CulTIvARES MAllORquINES DE AlMENDRO.-El examen de la diversidad alélica del locus S en diez cultivares mallorquines de almendro ha mostrado la presencia del alelo S f en cuatro de ellos, así como la presencia de cinco nuevos alelos no identificados hasta ahora. Aunque el alelo S f se ha descrito como responsable de la expresión de la autocompatibilidad del almendro, recientemente se ha descubierto que tiene dos expresiones fenotípicas diferentes, por lo que no siempre se puede considerar un índice de la autocompatibilidad de los genotipos que lo presentan. El estudio de los cuatro cultivares mallorquines que presentan este alelo ha confirmado su autoincompatibilidad, de manera que este alelo se expresa en estos cultivares en su forma fenotípicamente activa, como también se ha comprobado en un grupo de cultivares del sur de Italia, lo que podría indicar una conexión entre estas dos poblaciones mediterráneas de almendro.Palabras clave: alelos; almendro; autocompatibilidad; locus S; Prunus amygdalus. Resum PECulIARITATS Al·lèlIquES DEl lOCuS S DE lES CulTIvARS MAllORquINES D'AMETllER.-L'examen de la diversitat al·lèlica del locus S en deu cultivars mallorquines d'ametller ha mostrat la presència de l'al·lel S f en quatre, així com la presència de cinc al·lels nous fins ara no identificats. Encara que l'al·lel S f s'ha descrit com a responsable de l'expressió de l'autocompatibilitat de l'ametller, fa poc que s'ha descobert que té dues expressions fenotípiques diferents, pel que no sempre es pot considerar un índex de l'autocompatibilitat dels genotips que el tenen. L'estudi de les quatre cultivars mallorquines que presenten aquest al·lel ha confirmat la seva autoincompatibilitat, de manera que aquest al·lel s'hi expressa en la seva forma fenotípicament activa, com també s'ha comprovat en un grup de cultivars del sud d'Itàlia, fet que podria indicar una connexió entre aquestes dues poblacions mediterrànies d'ametller.

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“…Molecular characterization of S-allele identity of crossing parents allows rapid prediction of incompatibility reaction. Currently, more than 50 S-alleles have been identified in almond (Ortega et al 2005(Ortega et al , 2006Boskovic et al 2007;Kodad et al 2008a;Mousavi et al 2011) using different molecular-based methods.…”

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Phylogenetic relationships among the first and second introns of selectedPrunus S-RNase genes

et al. 2015

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Phylogenetic relationships among the first and second introns of selected Prunus S-RNase genes. Can. J. Plant Sci. 95: 1145Á 1154. To identify and evaluate self-incompatible alleles in almonds and related germplasm, DNA from 15 Prunus species was amplified using two degenerate consensus primer pairs flanking first and second S-locus introns (PaConsI-FD'EMPc1ConsRD and EM-Pc2ConsFD'EM-Pc3ConsRD). Twenty-eight amplified PCR products were analyzed by automated sequencer capillary electrophoresis. Sequenced fragments were aligned against available Prunus S-locus sequences in the National Center for Biotechnology Information and S-alleles identities were determined. The phylogenetic relationships between S-alleles in the germplasm studied were determined by the homology between their sequences and dendrograms were obtained for each primer pair. The Maximum Likelihood (homology) ranged from 84 to 100%. Most sequences were similar to cultivated almond (Prunus dulcis) or to the European wild almond (P. webbii). Twenty-six alleles for the first and the second introns were registered in the database in the GenBank. Two sequences of the first and second introns, which were taken from Prunus nairica and had similarity in GenBank, were registered in the database under a common sequence of the first and second intron. Analysis of phylogenetic relationships (dendrograms) among S-alleles from wild almond species as well as S-alleles cluster relations showed most pairs of alleles well supported by bootstrap. Key words: Germplasm diversity, self-incompatibility, S-allele, PrunusRahemi, A., Gradziel T.M., Chaparro J.X., Folta, K.M., Taghavi, T., Fatahi, R., Ebadi, A. et Hassani, D. 2015. Liens phyloge´ne´tiques entre le premier et le deuxie`me intron des ge`nes de la S-RNase de certaines espe`ces du genre Prunus. Can. J. Plant Sci. 95: 1145Á1154. Les auteurs ont amplifie´l'ADN de 15 espe`ces du genre Prunus pour identifier puis e´valuer les alle`les auto-incompatibles dans l'amande et le mate´riel ge´ne´tique connexe. À cette fin, ils ont utilise´deux paires d'amorces consensuelles de´ge´ne´re´es, situe´es de part et d'autre du premier et du deuxie`me intron du locus-S (PaConsI-FD'EMPc1ConsRD et EM-Pc2ConsFD'EM-Pc3ConsRD). Vingt-huit produits amplifie´s de la PCR ont e´te´analyse´s par e´lectrophore`se capillaire a`se´quenc¸age automatique. Les fragments se´quence´s ont e´te´compare´s aux se´quences du locus-S du genre Prunus disponibles au National Center for Biotechnology Information et les alle`les ont e´te´identifie´s. Les liens phyloge´ne´tiques entre les alle`les-S du mate´riel ge´ne´tique a`l'e´tude ont e´te´e´tablis par homologie entre les se´quences, et les auteurs ont obtenu les dendrogrammes de chaque paire d'amorces. La fourchette de probabilite´maximale (homologie) varie de 84 a`100 pour cent. La plupart des se´quences ressemblent a`celles de l'amandier cultive´(Prunus dulcis) ou de l'amandier sauvage d'Europe (P. webbii). Vingt-six alle`les du premier et du deuxie`me intron ont e´te´enregistre´s dans la base de donne...

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Self-(in)compatibility of the almonds P. dulcis and P. webbii: detection and cloning of ‘wild-type S f ’ and new self-compatibility alleles encoding inactive S-RNases

Cited by 45 publications

References 35 publications

Almond

Almond

Peculiaritats al·lèliques del locus <em>S</em> de les cultivars mallorquines d’ametller

Phylogenetic relationships among the first and second introns of selectedPrunus S-RNase genes

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